Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein
In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were el...
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| Veröffentlicht in: | International Journal of Industrial Entomology 2007-03, Vol.14 (1) |
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| creator | Zheng, Gui Zhong (Dong-A University, Busan, Republic of Korea) Kim, B.Y. (Dong-A University, Busan, Republic of Korea) Yoon, H.J. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) Wei, Ya Dong (Dong-A University, Busan, Republic of Korea) Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China) Jin, B.R. (Dong-A University, Busan, Republic of Korea) Shon, H.D. (Dong-A University, Busan, Republic of Korea), E-mail: hdsohn@dau.ac.kr |
| description | In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study. |
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(Dong-A University, Busan, Republic of Korea) ; Yoon, H.J. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) ; Wei, Ya Dong (Dong-A University, Busan, Republic of Korea) ; Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China) ; Jin, B.R. (Dong-A University, Busan, Republic of Korea) ; Shon, H.D. (Dong-A University, Busan, Republic of Korea), E-mail: hdsohn@dau.ac.kr</creator><creatorcontrib>Zheng, Gui Zhong (Dong-A University, Busan, Republic of Korea) ; Kim, B.Y. (Dong-A University, Busan, Republic of Korea) ; Yoon, H.J. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) ; Wei, Ya Dong (Dong-A University, Busan, Republic of Korea) ; Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China) ; Jin, B.R. (Dong-A University, Busan, Republic of Korea) ; Shon, H.D. (Dong-A University, Busan, Republic of Korea), E-mail: hdsohn@dau.ac.kr</creatorcontrib><description>In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study.</description><identifier>ISSN: 1598-3579</identifier><language>eng</language><subject>Apriona germari ; cDNA cloning ; Cuticle protein ; Gene structure ; INSECTA ; mRNA expression ; Mulberry longicorn beetle</subject><ispartof>International Journal of Industrial Entomology, 2007-03, Vol.14 (1)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Zheng, Gui Zhong (Dong-A University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Kim, B.Y. (Dong-A University, Busan, Republic of Korea)</creatorcontrib><creatorcontrib>Yoon, H.J. 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In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study.</description><subject>Apriona germari</subject><subject>cDNA cloning</subject><subject>Cuticle protein</subject><subject>Gene structure</subject><subject>INSECTA</subject><subject>mRNA expression</subject><subject>Mulberry longicorn beetle</subject><issn>1598-3579</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFjcsKwjAQRbNQsD4-QZgfEKLFPtyV-gJFRN3LEKc10iYySf0Iv1oEdwquzuKey2mJYDxNk1E4jdOO6Dp3kzIaT-IoEM_sztoahJK4RtawRX5gBXnjtaoI9mw9aQMrMuRmb9haKzh6bpRvmMAWcLoy0e8HoLmAmu8yyCtrtCnfPsLOPuir0RftAitHgw97YrhcnPL1qEB7xpK1O28OEyljKcM0icJ_-wslQUwy</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>Zheng, Gui Zhong (Dong-A University, Busan, Republic of Korea)</creator><creator>Kim, B.Y. (Dong-A University, Busan, Republic of Korea)</creator><creator>Yoon, H.J. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)</creator><creator>Wei, Ya Dong (Dong-A University, Busan, Republic of Korea)</creator><creator>Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China)</creator><creator>Jin, B.R. (Dong-A University, Busan, Republic of Korea)</creator><creator>Shon, H.D. (Dong-A University, Busan, Republic of Korea), E-mail: hdsohn@dau.ac.kr</creator><scope>FBQ</scope></search><sort><creationdate>200703</creationdate><title>Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein</title><author>Zheng, Gui Zhong (Dong-A University, Busan, Republic of Korea) ; Kim, B.Y. (Dong-A University, Busan, Republic of Korea) ; Yoon, H.J. (National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea) ; Wei, Ya Dong (Dong-A University, Busan, Republic of Korea) ; Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China) ; Jin, B.R. (Dong-A University, Busan, Republic of Korea) ; Shon, H.D. 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(National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea)</au><au>Wei, Ya Dong (Dong-A University, Busan, Republic of Korea)</au><au>Xijie, Guo (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China)</au><au>Jin, B.R. (Dong-A University, Busan, Republic of Korea)</au><au>Shon, H.D. (Dong-A University, Busan, Republic of Korea), E-mail: hdsohn@dau.ac.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein</atitle><jtitle>International Journal of Industrial Entomology</jtitle><date>2007-03</date><risdate>2007</risdate><volume>14</volume><issue>1</issue><issn>1598-3579</issn><abstract>In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study.</abstract></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; KoreaScience |
| subjects | Apriona germari cDNA cloning Cuticle protein Gene structure INSECTA mRNA expression Mulberry longicorn beetle |
| title | Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein |
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