Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes
Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'A...
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| Veröffentlicht in: | Journal of plant biotechnology 2005-09, Vol.7 (3) |
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| container_title | Journal of plant biotechnology |
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| creator | Zhao, Mei-Ai (Kangwon National University, Chuncheon, Republic of Korea) Dhital, Shambhu P. (Kangwon National University, Chuncheon, Republic of Korea) Fang, Yi-Lan (Kangwon National University, Chuncheon, Republic of Korea) Khu, D.M. (Kangwon National University, Chuncheon, Republic of Korea) Song, Y.S. (Kangwon National University, Chuncheon, Republic of Korea) Park, E.J. (Kangwon National University, Chuncheon, Republic of Korea) Kang, C.W. (Kangwon National University, Chuncheon, Republic of Korea) Lim, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: potatokorea@empal.com |
| description | Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior', 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to -40℃ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. |
| format | Article |
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(Kangwon National University, Chuncheon, Republic of Korea) ; Fang, Yi-Lan (Kangwon National University, Chuncheon, Republic of Korea) ; Khu, D.M. (Kangwon National University, Chuncheon, Republic of Korea) ; Song, Y.S. (Kangwon National University, Chuncheon, Republic of Korea) ; Park, E.J. (Kangwon National University, Chuncheon, Republic of Korea) ; Kang, C.W. (Kangwon National University, Chuncheon, Republic of Korea) ; Lim, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: potatokorea@empal.com</creator><creatorcontrib>Zhao, Mei-Ai (Kangwon National University, Chuncheon, Republic of Korea) ; Dhital, Shambhu P. (Kangwon National University, Chuncheon, Republic of Korea) ; Fang, Yi-Lan (Kangwon National University, Chuncheon, Republic of Korea) ; Khu, D.M. (Kangwon National University, Chuncheon, Republic of Korea) ; Song, Y.S. (Kangwon National University, Chuncheon, Republic of Korea) ; Park, E.J. (Kangwon National University, Chuncheon, Republic of Korea) ; Kang, C.W. (Kangwon National University, Chuncheon, Republic of Korea) ; Lim, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: potatokorea@empal.com</creatorcontrib><description>Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. 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(Kangwon National University, Chuncheon, Republic of Korea)</au><au>Song, Y.S. (Kangwon National University, Chuncheon, Republic of Korea)</au><au>Park, E.J. (Kangwon National University, Chuncheon, Republic of Korea)</au><au>Kang, C.W. (Kangwon National University, Chuncheon, Republic of Korea)</au><au>Lim, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: potatokorea@empal.com</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes</atitle><jtitle>Journal of plant biotechnology</jtitle><date>2005-09</date><risdate>2005</risdate><volume>7</volume><issue>3</issue><issn>1229-2818</issn><eissn>2384-1397</eissn><abstract>Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior', 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to -40℃ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1229-2818 |
| ispartof | Journal of plant biotechnology, 2005-09, Vol.7 (3) |
| issn | 1229-2818 2384-1397 |
| language | eng |
| recordid | cdi_fao_agris_KR2006015402 |
| source | Alma/SFX Local Collection |
| subjects | cold hardening cooling speed DECONGELATION DESCONGELACION osmoprotection REGENERACION REGENERATION THAWING VITRIFICACION VITRIFICATION |
| title | Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes |
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