Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'A...

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Veröffentlicht in:Journal of plant biotechnology 2005-09, Vol.7 (3)
Hauptverfasser: Zhao, Mei-Ai (Kangwon National University, Chuncheon, Republic of Korea), Dhital, Shambhu P. (Kangwon National University, Chuncheon, Republic of Korea), Fang, Yi-Lan (Kangwon National University, Chuncheon, Republic of Korea), Khu, D.M. (Kangwon National University, Chuncheon, Republic of Korea), Song, Y.S. (Kangwon National University, Chuncheon, Republic of Korea), Park, E.J. (Kangwon National University, Chuncheon, Republic of Korea), Kang, C.W. (Kangwon National University, Chuncheon, Republic of Korea), Lim, H.T. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: potatokorea@empal.com
Format: Artikel
Sprache:eng
Schlagworte:
cold hardening
cooling speed
DECONGELATION
DESCONGELACION
osmoprotection
REGENERACION
REGENERATION
THAWING
VITRIFICACION
VITRIFICATION
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Zusammenfassung:Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior', 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to -40℃ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium.
ISSN:1229-2818
2384-1397