Genetic and molecular analysis of the rpoD gene from Lactococcus lactis
A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal σ factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homo...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993-01, Vol.57 (1), p.88-92 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology) Ishibashi, N Shimamura, S Tanaka, K Takahashi, H |
| description | A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal σ factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis σ
43
factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation. |
| doi_str_mv | 10.1271/bbb.57.88 |
| format | Article |
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43
factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.57.88</identifier><identifier>PMID: 7503808</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Amino Acid Sequence ; Bacillus subtilis - chemistry ; Bacillus subtilis - genetics ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; BIOLOGIA MOLECULAR ; Biological and medical sciences ; BIOLOGIE MOLECULAIRE ; Biotechnology ; Blotting, Southern ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; DNA Primase ; DNA-Directed RNA Polymerases - chemistry ; DNA-Directed RNA Polymerases - genetics ; Escherichia coli - chemistry ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Bacterial ; Genetic Complementation Test ; GENETIC ENGINEERING ; Genetics ; GENIE GENETIQUE ; INGENIERIA GENETICA ; LACTOCOCCUS LACTIS ; Lactococcus lactis - chemistry ; Lactococcus lactis - genetics ; Microbiology ; MOLECULAR BIOLOGY ; Molecular Sequence Data ; Mutation ; Operon ; Restriction Mapping ; RNA Nucleotidyltransferases - chemistry ; RNA Nucleotidyltransferases - genetics ; Sigma Factor - chemistry ; Sigma Factor - genetics ; Temperature</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1993-01, Vol.57 (1), p.88-92</ispartof><rights>Copyright 1993 Taylor and Francis Group LLC 1993</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-787d2ac576dadd44091c185da69f8119c46527b53f4caf16a2a40068720205173</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4929460$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7503808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology)</creatorcontrib><creatorcontrib>Ishibashi, N</creatorcontrib><creatorcontrib>Shimamura, S</creatorcontrib><creatorcontrib>Tanaka, K</creatorcontrib><creatorcontrib>Takahashi, H</creatorcontrib><title>Genetic and molecular analysis of the rpoD gene from Lactococcus lactis</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal σ factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis σ
43
factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.</description><subject>Amino Acid Sequence</subject><subject>Bacillus subtilis - chemistry</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>BIOLOGIA MOLECULAR</subject><subject>Biological and medical sciences</subject><subject>BIOLOGIE MOLECULAIRE</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>DNA Primase</subject><subject>DNA-Directed RNA Polymerases - chemistry</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>GENETIC ENGINEERING</subject><subject>Genetics</subject><subject>GENIE GENETIQUE</subject><subject>INGENIERIA GENETICA</subject><subject>LACTOCOCCUS LACTIS</subject><subject>Lactococcus lactis - chemistry</subject><subject>Lactococcus lactis - genetics</subject><subject>Microbiology</subject><subject>MOLECULAR BIOLOGY</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Operon</subject><subject>Restriction Mapping</subject><subject>RNA Nucleotidyltransferases - chemistry</subject><subject>RNA Nucleotidyltransferases - genetics</subject><subject>Sigma Factor - chemistry</subject><subject>Sigma Factor - genetics</subject><subject>Temperature</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1rFDEUAPAglrpdPXgVhAFF8DBrvj-Opda1ZUEPeg5vMkmdkpmsyQyy_30ju_Yggqd8vF_eS_IQeknwhlBFPnRdtxFqo_UTtCKMq1Yarp6iFTZEtpoL8gxdlHKPcd0Q5BydK4GZxnqFtls_-XlwDUx9M6bo3RIh1xXEQxlKk0Iz__BN3qePzV2lTchpbHbg5uSSc0tpYp0P5Tk6CxCLf3Ea1-j7p-tvV5_b3ZftzdXlrnWCsblVWvUUnFCyh77nvN7HES16kCZoQozjUlDVCRa4g0AkUOAYS60oplgQxdbo3THvPqefiy-zHYfifIww-bQUq4TQTGv6X0ikVFxTU-Gbv-B9WnJ9fzWcG2akxKyq90flciol-2D3eRghHyzB9ncPbO2BFcpqXe3rU8alG33_KE-fXuNvT3EoDmLIMLmhPDJuqOG15hrxIxumkPIIv1KOvZ3hEFP-c4b9q_qr47EAycJdrur2q-GYUkHYA9P8pd8</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology)</creator><creator>Ishibashi, N</creator><creator>Shimamura, S</creator><creator>Tanaka, K</creator><creator>Takahashi, H</creator><general>Taylor & Francis</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Genetic and molecular analysis of the rpoD gene from Lactococcus lactis</title><author>Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology) ; Ishibashi, N ; Shimamura, S ; Tanaka, K ; Takahashi, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-787d2ac576dadd44091c185da69f8119c46527b53f4caf16a2a40068720205173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Bacillus subtilis - chemistry</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>BIOLOGIA MOLECULAR</topic><topic>Biological and medical sciences</topic><topic>BIOLOGIE MOLECULAIRE</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>DNA Primase</topic><topic>DNA-Directed RNA Polymerases - chemistry</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>GENETIC ENGINEERING</topic><topic>Genetics</topic><topic>GENIE GENETIQUE</topic><topic>INGENIERIA GENETICA</topic><topic>LACTOCOCCUS LACTIS</topic><topic>Lactococcus lactis - chemistry</topic><topic>Lactococcus lactis - genetics</topic><topic>Microbiology</topic><topic>MOLECULAR BIOLOGY</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Operon</topic><topic>Restriction Mapping</topic><topic>RNA Nucleotidyltransferases - chemistry</topic><topic>RNA Nucleotidyltransferases - genetics</topic><topic>Sigma Factor - chemistry</topic><topic>Sigma Factor - genetics</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology)</creatorcontrib><creatorcontrib>Ishibashi, N</creatorcontrib><creatorcontrib>Shimamura, S</creatorcontrib><creatorcontrib>Tanaka, K</creatorcontrib><creatorcontrib>Takahashi, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araya, T. (Tokyo Univ. (Japan). Inst. of Applied Microbiology)</au><au>Ishibashi, N</au><au>Shimamura, S</au><au>Tanaka, K</au><au>Takahashi, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and molecular analysis of the rpoD gene from Lactococcus lactis</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1993-01</date><risdate>1993</risdate><volume>57</volume><issue>1</issue><spage>88</spage><epage>92</epage><pages>88-92</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal σ factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis σ
43
factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.</abstract><cop>Tokyo</cop><pub>Taylor & Francis</pub><pmid>7503808</pmid><doi>10.1271/bbb.57.88</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1993-01, Vol.57 (1), p.88-92 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_fao_agris_JP9402251 |
| source | MEDLINE; Full-Text Journals in Chemistry (Open access); J-STAGE; Freely Accessible Japanese Titles (ERDB Project); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence Bacillus subtilis - chemistry Bacillus subtilis - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacteriology Base Sequence BIOLOGIA MOLECULAR Biological and medical sciences BIOLOGIE MOLECULAIRE Biotechnology Blotting, Southern CLONACION CLONAGE CLONING Cloning, Molecular DNA Primase DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - genetics Escherichia coli - chemistry Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genes, Bacterial Genetic Complementation Test GENETIC ENGINEERING Genetics GENIE GENETIQUE INGENIERIA GENETICA LACTOCOCCUS LACTIS Lactococcus lactis - chemistry Lactococcus lactis - genetics Microbiology MOLECULAR BIOLOGY Molecular Sequence Data Mutation Operon Restriction Mapping RNA Nucleotidyltransferases - chemistry RNA Nucleotidyltransferases - genetics Sigma Factor - chemistry Sigma Factor - genetics Temperature |
| title | Genetic and molecular analysis of the rpoD gene from Lactococcus lactis |
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