An alkaline magnesium dependent inorganic pyrophosphatase from the leaf of Amarantus blitum L
The purified inorganic pyrophosphatase from the leaf of Amarantus blitum shows absolute magnesium requirement for its enzyme activity. For maximum enzyme activity, the Mg : PP i ratio was found to be 10:1 at the optimum pH of 9.0. This ratio varies with the shift of the pH. was found to be the true...
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| Veröffentlicht in: | Agricultural and biological chemistry 1983-06, Vol.47 (6), p.1341-1344 |
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| container_title | Agricultural and biological chemistry |
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| creator | Mukherjee, J.J Pal, P.R. (Indian Statistical Inst., Calcutta (India)) |
| description | The purified inorganic pyrophosphatase from the leaf of Amarantus blitum shows absolute magnesium requirement for its enzyme activity. For maximum enzyme activity, the Mg : PP
i
ratio was found to be 10:1 at the optimum pH of 9.0. This ratio varies with the shift of the pH.
was found to be the true substrate for the enzyme in the presence of the free Mg
2+
ion. Some divalent metal ions and the fluoride anion severely inhibit the enzyme activity in the presence of Mg
2+
. The Michaelis constant (Km) and molecular weight of the enzyme were found to be 4.95·
−6
m and 32,860, respectively. This enzyme is strictly specific for inorganic pyrophosphate. |
| doi_str_mv | 10.1080/00021369.1983.10866080 |
| format | Article |
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i
ratio was found to be 10:1 at the optimum pH of 9.0. This ratio varies with the shift of the pH.
was found to be the true substrate for the enzyme in the presence of the free Mg
2+
ion. Some divalent metal ions and the fluoride anion severely inhibit the enzyme activity in the presence of Mg
2+
. The Michaelis constant (Km) and molecular weight of the enzyme were found to be 4.95·
−6
m and 32,860, respectively. This enzyme is strictly specific for inorganic pyrophosphate.</description><identifier>ISSN: 0002-1369</identifier><identifier>DOI: 10.1080/00021369.1983.10866080</identifier><language>eng</language><publisher>Taylor & Francis</publisher><subject>AMARANTHUS ; Amarantus blitum ; FEUILLE ; HIDROLASAS ; HOJAS ; HYDROLASE ; HYDROLASES ; inorganic pyrophosphatase ; LEAVES ; MAGNESIO ; MAGNESIUM</subject><ispartof>Agricultural and biological chemistry, 1983-06, Vol.47 (6), p.1341-1344</ispartof><rights>Copyright, 1983, by the Agricultural Chemical Society of Japan 1983</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-d3a3cc98aee7861d926e95e0b3854addb52fe062528a66201d9a61a783dbaf033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Mukherjee, J.J</creatorcontrib><creatorcontrib>Pal, P.R. (Indian Statistical Inst., Calcutta (India))</creatorcontrib><title>An alkaline magnesium dependent inorganic pyrophosphatase from the leaf of Amarantus blitum L</title><title>Agricultural and biological chemistry</title><description>The purified inorganic pyrophosphatase from the leaf of Amarantus blitum shows absolute magnesium requirement for its enzyme activity. For maximum enzyme activity, the Mg : PP
i
ratio was found to be 10:1 at the optimum pH of 9.0. This ratio varies with the shift of the pH.
was found to be the true substrate for the enzyme in the presence of the free Mg
2+
ion. Some divalent metal ions and the fluoride anion severely inhibit the enzyme activity in the presence of Mg
2+
. The Michaelis constant (Km) and molecular weight of the enzyme were found to be 4.95·
−6
m and 32,860, respectively. This enzyme is strictly specific for inorganic pyrophosphate.</description><subject>AMARANTHUS</subject><subject>Amarantus blitum</subject><subject>FEUILLE</subject><subject>HIDROLASAS</subject><subject>HOJAS</subject><subject>HYDROLASE</subject><subject>HYDROLASES</subject><subject>inorganic pyrophosphatase</subject><subject>LEAVES</subject><subject>MAGNESIO</subject><subject>MAGNESIUM</subject><issn>0002-1369</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNqFUDtPwzAQzgASpfAHGJAntpZL3LjOWFU8VQkGGJF1Tc6twbGDnQr13-OodEY3nPS9dPdl2XUO0xwk3AJAkXNRTfNK8gESIsEn2WggJgNzlp3H-AlQAgcYZR8Lx9B-oTWOWIsbR9HsWtZQR64h1zPjfNigMzXr9sF3Wx-7LfYYiengW9ZviVlCzbxmixYDun4X2dqaPqWsLrJTjTbS5d8eZ-_3d2_Lx8nq5eFpuVhNai54P2k48rquJBLNpcibqhBUlQRrLssZNs26LDSBKMpCohAFJAWKHOeSN2vUwPk4uznkdsF_7yj2qjWxJmvRkd9FlfN5KWczSEJxENbBxxhIqy6YdPZe5aCGBtWxQTU0qI4NJuPiYDRO-9Dijw-2UT3urQ86fV2bqPi_GVeHDI1e4SYky_OrnEGakv8CCf6DQw</recordid><startdate>19830601</startdate><enddate>19830601</enddate><creator>Mukherjee, J.J</creator><creator>Pal, P.R. (Indian Statistical Inst., Calcutta (India))</creator><general>Taylor & Francis</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19830601</creationdate><title>An alkaline magnesium dependent inorganic pyrophosphatase from the leaf of Amarantus blitum L</title><author>Mukherjee, J.J ; Pal, P.R. (Indian Statistical Inst., Calcutta (India))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-d3a3cc98aee7861d926e95e0b3854addb52fe062528a66201d9a61a783dbaf033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>AMARANTHUS</topic><topic>Amarantus blitum</topic><topic>FEUILLE</topic><topic>HIDROLASAS</topic><topic>HOJAS</topic><topic>HYDROLASE</topic><topic>HYDROLASES</topic><topic>inorganic pyrophosphatase</topic><topic>LEAVES</topic><topic>MAGNESIO</topic><topic>MAGNESIUM</topic><toplevel>online_resources</toplevel><creatorcontrib>Mukherjee, J.J</creatorcontrib><creatorcontrib>Pal, P.R. (Indian Statistical Inst., Calcutta (India))</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Agricultural and biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mukherjee, J.J</au><au>Pal, P.R. (Indian Statistical Inst., Calcutta (India))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An alkaline magnesium dependent inorganic pyrophosphatase from the leaf of Amarantus blitum L</atitle><jtitle>Agricultural and biological chemistry</jtitle><date>1983-06-01</date><risdate>1983</risdate><volume>47</volume><issue>6</issue><spage>1341</spage><epage>1344</epage><pages>1341-1344</pages><issn>0002-1369</issn><abstract>The purified inorganic pyrophosphatase from the leaf of Amarantus blitum shows absolute magnesium requirement for its enzyme activity. For maximum enzyme activity, the Mg : PP
i
ratio was found to be 10:1 at the optimum pH of 9.0. This ratio varies with the shift of the pH.
was found to be the true substrate for the enzyme in the presence of the free Mg
2+
ion. Some divalent metal ions and the fluoride anion severely inhibit the enzyme activity in the presence of Mg
2+
. The Michaelis constant (Km) and molecular weight of the enzyme were found to be 4.95·
−6
m and 32,860, respectively. This enzyme is strictly specific for inorganic pyrophosphate.</abstract><pub>Taylor & Francis</pub><doi>10.1080/00021369.1983.10866080</doi><tpages>4</tpages></addata></record> |
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| ispartof | Agricultural and biological chemistry, 1983-06, Vol.47 (6), p.1341-1344 |
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| language | eng |
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| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | AMARANTHUS Amarantus blitum FEUILLE HIDROLASAS HOJAS HYDROLASE HYDROLASES inorganic pyrophosphatase LEAVES MAGNESIO MAGNESIUM |
| title | An alkaline magnesium dependent inorganic pyrophosphatase from the leaf of Amarantus blitum L |
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