Verification of the active bovine viral diarrhea virus (BVDV) contaminated in bovine live viral vaccines by means of the detection of negative-sense RNA of BVDV with RT-PCR

The reverse transcription polymerase chain reaction (RT-PCR) developed for the detection of active pestiviruses by amplification of negative strand viral RNA was verified to detect extraneous active bovine viral diarrhea virus (BVDV) in bovine live viral vaccines. The novel method was confirmed to b...

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Veröffentlicht in:Japanese Journal of Animal Hygiene (Japan) 2009-09, Vol.35 (2)
Hauptverfasser: Shimazaki, T.(Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.), Takahashi, C, Aoki, H, Suzuki, S, Fukusho, A
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Sprache:eng
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