Glutamyl tRNA synthetases and glutamic acid induce sexual differentiation of Schizosaccharomyces pombe
The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA sy...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1339-1347 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Paul, S.K.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science) Goldar, Md.M Yakura, M Oowatari, Y Kawamukai, M |
| description | The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA synthetase (cGluRS) also interacted with Moc3 in a yeast two-hybrid system. Disruption of ers1 (cGluRS) and of ers2 (mGluRS) indicated that these genes are both essential for the cell growth of S. pombe. We found that ers2 severely affected cell growth and decreased viability, but induced sexual differentiation of S. pombe when it was over-expressed. Over-expression of ers1 also stimulated sexual differentiation in S. pombe. These observations led us to test the effects of various amino acids on sexual differentiation. We found that glutamic acid, as well as other specific amino acids, such as tryptophan, methionine, and threonine, efficiently induced sexual differentiation in S. pombe. Our findings suggest a new regulatory mechanism where GluRSs and glutamic acid are involved in sexual differentiation in S. pombe. |
| doi_str_mv | 10.1271/bbb.90005 |
| format | Article |
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Faculty of Life and Environmental Science) ; Goldar, Md.M ; Yakura, M ; Oowatari, Y ; Kawamukai, M</creator><creatorcontrib>Paul, S.K.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science) ; Goldar, Md.M ; Yakura, M ; Oowatari, Y ; Kawamukai, M</creatorcontrib><description>The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA synthetase (cGluRS) also interacted with Moc3 in a yeast two-hybrid system. Disruption of ers1 (cGluRS) and of ers2 (mGluRS) indicated that these genes are both essential for the cell growth of S. pombe. We found that ers2 severely affected cell growth and decreased viability, but induced sexual differentiation of S. pombe when it was over-expressed. Over-expression of ers1 also stimulated sexual differentiation in S. pombe. These observations led us to test the effects of various amino acids on sexual differentiation. We found that glutamic acid, as well as other specific amino acids, such as tryptophan, methionine, and threonine, efficiently induced sexual differentiation in S. pombe. Our findings suggest a new regulatory mechanism where GluRSs and glutamic acid are involved in sexual differentiation in S. pombe.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.90005</identifier><identifier>PMID: 19502729</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ACIDE GLUTAMIQUE ; ACIDO GLUTAMICO ; AISLAMIENTO ; ARN DE TRANSFERENCIA ; ARN DE TRANSFERT ; Base Sequence ; Biological and medical sciences ; CELLS ; CELLULE ; CELULAS ; CHEMICAL COMPOSITION ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; CRECIMIENTO ; CROISSANCE ; DIFERENCIACION SEXUAL ; DIFFERENCIATION SEXUELLE ; DNA Primers ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Fungal ; GluRS ; Glutamate-tRNA Ligase - genetics ; Glutamate-tRNA Ligase - physiology ; GLUTAMIC ACID ; Glutamic Acid - physiology ; GROWTH ; ISOLATION ; ISOLEMENT ; MITOCHONDRIA ; MITOCHONDRIE ; MITOCONDRIA ; Schizosaccharomyces - enzymology ; Schizosaccharomyces - genetics ; Schizosaccharomyces - growth & development ; Schizosaccharomyces - physiology ; SCHIZOSACCHAROMYCES POMBE ; SEX DIFFERENTIATION ; sexual differentiation ; TRANSFER RNA ; Two-Hybrid System Techniques</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1339-1347</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c676t-35bc8ba12642d0ace195220d6c0750ca1a22b7b2ebcc7f446ab15d85949d3b2a3</citedby><cites>FETCH-LOGICAL-c676t-35bc8ba12642d0ace195220d6c0750ca1a22b7b2ebcc7f446ab15d85949d3b2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21794036$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19502729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paul, S.K.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science)</creatorcontrib><creatorcontrib>Goldar, Md.M</creatorcontrib><creatorcontrib>Yakura, M</creatorcontrib><creatorcontrib>Oowatari, Y</creatorcontrib><creatorcontrib>Kawamukai, M</creatorcontrib><title>Glutamyl tRNA synthetases and glutamic acid induce sexual differentiation of Schizosaccharomyces pombe</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA synthetase (cGluRS) also interacted with Moc3 in a yeast two-hybrid system. Disruption of ers1 (cGluRS) and of ers2 (mGluRS) indicated that these genes are both essential for the cell growth of S. pombe. We found that ers2 severely affected cell growth and decreased viability, but induced sexual differentiation of S. pombe when it was over-expressed. Over-expression of ers1 also stimulated sexual differentiation in S. pombe. These observations led us to test the effects of various amino acids on sexual differentiation. We found that glutamic acid, as well as other specific amino acids, such as tryptophan, methionine, and threonine, efficiently induced sexual differentiation in S. pombe. Our findings suggest a new regulatory mechanism where GluRSs and glutamic acid are involved in sexual differentiation in S. pombe.</description><subject>ACIDE GLUTAMIQUE</subject><subject>ACIDO GLUTAMICO</subject><subject>AISLAMIENTO</subject><subject>ARN DE TRANSFERENCIA</subject><subject>ARN DE TRANSFERT</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CELLS</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CHEMICAL COMPOSITION</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>DIFERENCIACION SEXUAL</subject><subject>DIFFERENCIATION SEXUELLE</subject><subject>DNA Primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Fungal</subject><subject>GluRS</subject><subject>Glutamate-tRNA Ligase - genetics</subject><subject>Glutamate-tRNA Ligase - physiology</subject><subject>GLUTAMIC ACID</subject><subject>Glutamic Acid - physiology</subject><subject>GROWTH</subject><subject>ISOLATION</subject><subject>ISOLEMENT</subject><subject>MITOCHONDRIA</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>Schizosaccharomyces - enzymology</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - growth & development</subject><subject>Schizosaccharomyces - physiology</subject><subject>SCHIZOSACCHAROMYCES POMBE</subject><subject>SEX DIFFERENTIATION</subject><subject>sexual differentiation</subject><subject>TRANSFER RNA</subject><subject>Two-Hybrid System Techniques</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ktrFTEUB_Agir1WF34AJSAKLqbmNcnMshStSlHxsQ4nr96Umck1mUHHT2_ae1UQwVUW-Z2THP4HoYeUnFCm6AtjzElPCGlvoQ3lQjWyF-o22pCeyqYTLT1C90q5qqKnLb2LjmjfEqZYv0HhfFhmGNcBzx_fneKyTvPWz1B8wTA5fHlzGy0GGx2Ok1usx8V_X2DALobgs5_mCHNME04Bf7Lb-CMVsHYLOY2rrW12aTT-ProTYCj-weE8Rl9evfx89rq5eH_-5uz0orFSybnhrbGdAcqkYI6A9fWjjBEnLVEtsUCBMaMM88ZaFYSQYGjrurYXveOGAT9Gz_Z9dzl9XXyZ9RiL9cMAk09L0VIJ2gop_wsZoYSTrqvwyV_wKi15qkNoKkTfCcY5rer5XtmcSsk-6F2OI-RVU6KvM9I1I32TUbWPDx0XM3r3Rx5CqeDpAUCxMIQMk43lt2NU9YLw6xnE3sUppDzCt5QHp2dYh5R_FfF_vf9oXxYgabjMVb39wOpuECIEU_wnbeG0TQ</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Paul, S.K.(Shimane Univ., Matsue (Japan). 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Faculty of Life and Environmental Science) ; Goldar, Md.M ; Yakura, M ; Oowatari, Y ; Kawamukai, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c676t-35bc8ba12642d0ace195220d6c0750ca1a22b7b2ebcc7f446ab15d85949d3b2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ACIDE GLUTAMIQUE</topic><topic>ACIDO GLUTAMICO</topic><topic>AISLAMIENTO</topic><topic>ARN DE TRANSFERENCIA</topic><topic>ARN DE TRANSFERT</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CELLS</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CHEMICAL COMPOSITION</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>CRECIMIENTO</topic><topic>CROISSANCE</topic><topic>DIFERENCIACION SEXUAL</topic><topic>DIFFERENCIATION SEXUELLE</topic><topic>DNA Primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Fungal</topic><topic>GluRS</topic><topic>Glutamate-tRNA Ligase - genetics</topic><topic>Glutamate-tRNA Ligase - physiology</topic><topic>GLUTAMIC ACID</topic><topic>Glutamic Acid - physiology</topic><topic>GROWTH</topic><topic>ISOLATION</topic><topic>ISOLEMENT</topic><topic>MITOCHONDRIA</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Schizosaccharomyces - enzymology</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - growth & development</topic><topic>Schizosaccharomyces - physiology</topic><topic>SCHIZOSACCHAROMYCES POMBE</topic><topic>SEX DIFFERENTIATION</topic><topic>sexual differentiation</topic><topic>TRANSFER RNA</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paul, S.K.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science)</creatorcontrib><creatorcontrib>Goldar, Md.M</creatorcontrib><creatorcontrib>Yakura, M</creatorcontrib><creatorcontrib>Oowatari, Y</creatorcontrib><creatorcontrib>Kawamukai, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paul, S.K.(Shimane Univ., Matsue (Japan). Faculty of Life and Environmental Science)</au><au>Goldar, Md.M</au><au>Yakura, M</au><au>Oowatari, Y</au><au>Kawamukai, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutamyl tRNA synthetases and glutamic acid induce sexual differentiation of Schizosaccharomyces pombe</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>73</volume><issue>6</issue><spage>1339</spage><epage>1347</epage><pages>1339-1347</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA synthetase (cGluRS) also interacted with Moc3 in a yeast two-hybrid system. Disruption of ers1 (cGluRS) and of ers2 (mGluRS) indicated that these genes are both essential for the cell growth of S. pombe. We found that ers2 severely affected cell growth and decreased viability, but induced sexual differentiation of S. pombe when it was over-expressed. Over-expression of ers1 also stimulated sexual differentiation in S. pombe. These observations led us to test the effects of various amino acids on sexual differentiation. We found that glutamic acid, as well as other specific amino acids, such as tryptophan, methionine, and threonine, efficiently induced sexual differentiation in S. pombe. Our findings suggest a new regulatory mechanism where GluRSs and glutamic acid are involved in sexual differentiation in S. pombe.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>19502729</pmid><doi>10.1271/bbb.90005</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1339-1347 |
| issn | 0916-8451 1347-6947 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | ACIDE GLUTAMIQUE ACIDO GLUTAMICO AISLAMIENTO ARN DE TRANSFERENCIA ARN DE TRANSFERT Base Sequence Biological and medical sciences CELLS CELLULE CELULAS CHEMICAL COMPOSITION COMPOSICION QUIMICA COMPOSITION CHIMIQUE CRECIMIENTO CROISSANCE DIFERENCIACION SEXUAL DIFFERENCIATION SEXUELLE DNA Primers Fundamental and applied biological sciences. Psychology GENE GENES Genes, Fungal GluRS Glutamate-tRNA Ligase - genetics Glutamate-tRNA Ligase - physiology GLUTAMIC ACID Glutamic Acid - physiology GROWTH ISOLATION ISOLEMENT MITOCHONDRIA MITOCHONDRIE MITOCONDRIA Schizosaccharomyces - enzymology Schizosaccharomyces - genetics Schizosaccharomyces - growth & development Schizosaccharomyces - physiology SCHIZOSACCHAROMYCES POMBE SEX DIFFERENTIATION sexual differentiation TRANSFER RNA Two-Hybrid System Techniques |
| title | Glutamyl tRNA synthetases and glutamic acid induce sexual differentiation of Schizosaccharomyces pombe |
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