Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process
This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-02, Vol.72 (2), p.360-367 |
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| creator | Okumura, T.(Yakult Honsha Co. Ltd., Kunitachi, Tokyo (Japan). Yakult Central Inst. for Microbiological Research) Nagai, F Yamamoto, S Hashimoto, T Ito, M Sawada, H |
| description | This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0 x 10E5 (logsub(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field. |
| doi_str_mv | 10.1271/bbb.70427 |
| format | Article |
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Ltd., Kunitachi, Tokyo (Japan). Yakult Central Inst. for Microbiological Research) ; Nagai, F ; Yamamoto, S ; Hashimoto, T ; Ito, M ; Sawada, H</creator><creatorcontrib>Okumura, T.(Yakult Honsha Co. Ltd., Kunitachi, Tokyo (Japan). Yakult Central Inst. for Microbiological Research) ; Nagai, F ; Yamamoto, S ; Hashimoto, T ; Ito, M ; Sawada, H</creatorcontrib><description>This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0 x 10E5 (logsub(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.70427</identifier><identifier>PMID: 18256494</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ANTICORPS POLYCLONAL ; ANTICUERPOS POLICLONALES ; Bacteria - genetics ; Bacteria - isolation & purification ; BACTERIA FIJADORA DEL NITROGENO ; BACTERIE FIXATRICE DE L'AZOTE ; Base Sequence ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; DNA Primers ; Environment and pollution ; FOSFATOS (SALES) ; Fundamental and applied biological sciences. Psychology ; IMMUNOLOGICAL TECHNIQUES ; In Situ Hybridization, Fluorescence ; Industrial applications and implications. Economical aspects ; LATEX ; Latex Fixation Tests - methods ; NITROGEN FIXING BACTERIA ; Nitrospira ; PCR ; PHOSPHATE (SEL) ; PHOSPHATES (SALTS) ; POLYCLONAL ANTIBODIES ; Polymerase Chain Reaction ; quantification ; reverse passive latex agglutination (RPLA) ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS ; TRAITEMENT DES EAUX USEES ; TRATAMIENTO DE AGUAS RESIDUALES ; WASTEWATER TREATMENT ; wastewater treatment process ; Water Microbiology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2008-02, Vol.72 (2), p.360-367</ispartof><rights>2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008</rights><rights>2008 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20232911$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18256494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okumura, T.(Yakult Honsha Co. Ltd., Kunitachi, Tokyo (Japan). Yakult Central Inst. for Microbiological Research)</creatorcontrib><creatorcontrib>Nagai, F</creatorcontrib><creatorcontrib>Yamamoto, S</creatorcontrib><creatorcontrib>Hashimoto, T</creatorcontrib><creatorcontrib>Ito, M</creatorcontrib><creatorcontrib>Sawada, H</creatorcontrib><title>Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0 x 10E5 (logsub(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field.</description><subject>ANTICORPS POLYCLONAL</subject><subject>ANTICUERPOS POLICLONALES</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>BACTERIA FIJADORA DEL NITROGENO</subject><subject>BACTERIE FIXATRICE DE L'AZOTE</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>DNA Primers</subject><subject>Environment and pollution</subject><subject>FOSFATOS (SALES)</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Industrial applications and implications. 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Psychology</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>LATEX</topic><topic>Latex Fixation Tests - methods</topic><topic>NITROGEN FIXING BACTERIA</topic><topic>Nitrospira</topic><topic>PCR</topic><topic>PHOSPHATE (SEL)</topic><topic>PHOSPHATES (SALTS)</topic><topic>POLYCLONAL ANTIBODIES</topic><topic>Polymerase Chain Reaction</topic><topic>quantification</topic><topic>reverse passive latex agglutination (RPLA)</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><topic>TRAITEMENT DES EAUX USEES</topic><topic>TRATAMIENTO DE AGUAS RESIDUALES</topic><topic>WASTEWATER TREATMENT</topic><topic>wastewater treatment process</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okumura, T.(Yakult Honsha Co. Ltd., Kunitachi, Tokyo (Japan). 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Ltd., Kunitachi, Tokyo (Japan). Yakult Central Inst. for Microbiological Research)</au><au>Nagai, F</au><au>Yamamoto, S</au><au>Hashimoto, T</au><au>Ito, M</au><au>Sawada, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>72</volume><issue>2</issue><spage>360</spage><epage>367</epage><pages>360-367</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0 x 10E5 (logsub(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>18256494</pmid><doi>10.1271/bbb.70427</doi><tpages>8</tpages></addata></record> |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | ANTICORPS POLYCLONAL ANTICUERPOS POLICLONALES Bacteria - genetics Bacteria - isolation & purification BACTERIA FIJADORA DEL NITROGENO BACTERIE FIXATRICE DE L'AZOTE Base Sequence Biological and medical sciences Biological treatment of waters Biotechnology DNA Primers Environment and pollution FOSFATOS (SALES) Fundamental and applied biological sciences. Psychology IMMUNOLOGICAL TECHNIQUES In Situ Hybridization, Fluorescence Industrial applications and implications. Economical aspects LATEX Latex Fixation Tests - methods NITROGEN FIXING BACTERIA Nitrospira PCR PHOSPHATE (SEL) PHOSPHATES (SALTS) POLYCLONAL ANTIBODIES Polymerase Chain Reaction quantification reverse passive latex agglutination (RPLA) TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS TRAITEMENT DES EAUX USEES TRATAMIENTO DE AGUAS RESIDUALES WASTEWATER TREATMENT wastewater treatment process Water Microbiology |
| title | Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process |
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