CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato [Lycopersicon esculentum]

We characterized a new cytochrome P450 monooxygenase (P450), CYP724B2, from tomato (Lycopersicon esculentum). CYP724B2 showed 42% and 62% amino acid sequence identity with Arabidopsis DWARF4/CYP90B1 and rice DWARF11/CYP724Bl respectively. Functional assay of CYP724B2 heterologously expressed in inse...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-09, Vol.70 (9), p.2071-2080
Hauptverfasser:	Ohnishi, T.(Kyoto Univ. (Japan)), Watanabe, B, Sakata, K, Mizutani, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence AMINO ACID SEQUENCES Base Sequence BIOCHEMICAL PATHWAYS Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS BRASINOSTEROIDES brassinosteroid BRASSINOSTEROIDE BRASSINOSTEROIDS C-22 hydroxylation CITOCROMO P450 CYP724B CYP90B Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism CYTOCHROME P450 Expressed Sequence Tags - metabolism Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry HYDROXYLATION IDROXILACION Isoenzymes - genetics Isoenzymes - metabolism LYCOPERSICON ESCULENTUM Lycopersicon esculentum - enzymology Lycopersicon esculentum - genetics Lycopersicon esculentum - metabolism Molecular Sequence Data Phylogeny Phytosterols - biosynthesis Phytosterols - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Plant - chemistry RNA, Plant - genetics SECUENCIAS DE AMINOACIDOS Sequence Alignment Sequence Analysis, DNA SEQUENCE D'ACIDES AMINES Spectrophotometry, Ultraviolet VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME
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