Glycoform analysis of Japanese cedar [Cryptomeria japonica] pollen allergen, Cry j 1
In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoaller...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005, Vol.69 (9), p.1700-1705 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Maeda, M.(Okayama Univ. (Japan)) Kamamoto, M Hino, K Yamamoto, S Kimura, M Okano, M Kimura, Y |
| description | In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exoand endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESIMS). Structural analysis clearly indicated that Lewis a epitope (Gal beta l-3(Fuc alpha l4) GlcNAc beta 1-), instead of the Gal beta l-4(Fuc alpha l-6) GlcNAc, occurs in the N-glycans of Cry j 1. |
| doi_str_mv | 10.1271/bbb.69.1700 |
| format | Article |
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(Japan)) ; Kamamoto, M ; Hino, K ; Yamamoto, S ; Kimura, M ; Okano, M ; Kimura, Y</creator><creatorcontrib>Maeda, M.(Okayama Univ. (Japan)) ; Kamamoto, M ; Hino, K ; Yamamoto, S ; Kimura, M ; Okano, M ; Kimura, Y</creatorcontrib><description>In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exoand endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESIMS). Structural analysis clearly indicated that Lewis a epitope (Gal beta l-3(Fuc alpha l4) GlcNAc beta 1-), instead of the Gal beta l-4(Fuc alpha l-6) GlcNAc, occurs in the N-glycans of Cry j 1.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.69.1700</identifier><identifier>PMID: 16195587</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ALERGENOS ; ALLERGENE ; ALLERGENS ; Allergens - analysis ; Allergens - chemistry ; Antigens, Plant ; Biological and medical sciences ; Carbohydrate Sequence ; CHEMICAL STRUCTURE ; Cry j 1 ; Cryptomeria ; CRYPTOMERIA JAPONICA ; Epitopes - analysis ; Epitopes - chemistry ; ESTRUCTURA QUIMICA ; Fundamental and applied biological sciences. Psychology ; Glycoside Hydrolases - metabolism ; HYPERSENSITIVITY ; Japanese cedar pollen ; Lewis a epitope ; Molecular Sequence Data ; N-glycan ; Plant Proteins - analysis ; Plant Proteins - chemistry ; POLEN ; POLLEN ; Pollen - chemistry ; pollen allergen ; Polysaccharides - chemistry ; REACCIONES ALERGICAS ; REACTION ALLERGIQUE ; Spectrometry, Mass, Electrospray Ionization ; STRUCTURE CHIMIQUE</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2005, Vol.69 (9), p.1700-1705</ispartof><rights>2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2005</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c698t-12f81e44ab42bf9609bd4b2cc8423934b42148b9f815dc1de6e57697c3895e373</citedby><cites>FETCH-LOGICAL-c698t-12f81e44ab42bf9609bd4b2cc8423934b42148b9f815dc1de6e57697c3895e373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17316419$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16195587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maeda, M.(Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Kamamoto, M</creatorcontrib><creatorcontrib>Hino, K</creatorcontrib><creatorcontrib>Yamamoto, S</creatorcontrib><creatorcontrib>Kimura, M</creatorcontrib><creatorcontrib>Okano, M</creatorcontrib><creatorcontrib>Kimura, Y</creatorcontrib><title>Glycoform analysis of Japanese cedar [Cryptomeria japonica] pollen allergen, Cry j 1</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exoand endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESIMS). Structural analysis clearly indicated that Lewis a epitope (Gal beta l-3(Fuc alpha l4) GlcNAc beta 1-), instead of the Gal beta l-4(Fuc alpha l-6) GlcNAc, occurs in the N-glycans of Cry j 1.</description><subject>ALERGENOS</subject><subject>ALLERGENE</subject><subject>ALLERGENS</subject><subject>Allergens - analysis</subject><subject>Allergens - chemistry</subject><subject>Antigens, Plant</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Sequence</subject><subject>CHEMICAL STRUCTURE</subject><subject>Cry j 1</subject><subject>Cryptomeria</subject><subject>CRYPTOMERIA JAPONICA</subject><subject>Epitopes - analysis</subject><subject>Epitopes - chemistry</subject><subject>ESTRUCTURA QUIMICA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>HYPERSENSITIVITY</subject><subject>Japanese cedar pollen</subject><subject>Lewis a epitope</subject><subject>Molecular Sequence Data</subject><subject>N-glycan</subject><subject>Plant Proteins - analysis</subject><subject>Plant Proteins - chemistry</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Pollen - chemistry</subject><subject>pollen allergen</subject><subject>Polysaccharides - chemistry</subject><subject>REACCIONES ALERGICAS</subject><subject>REACTION ALLERGIQUE</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>STRUCTURE CHIMIQUE</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9rFDEUB_Agit1WT56VgOhFZ82b_D7KUquloId6EglJJlNmyUymyS4y_71ZdqUggpcEHp_3eLwvQi-ArKGV8ME5txZ6DZKQR2gFlMlGaCYfoxXRIBrFOJyh81K2hNQCh6foDARozpVcoduruPjUpzxiO9m4lKHg1ONrO9splIB96GzGPzZ5mXdpDHmweGvnNA3e_sRzijFM2NY334XpPa4MbzE8Q096G0t4fvov0PdPl7ebz83N16svm483jRda7RpoewWBMetY63otiHYdc633irVUU1bLwJTTVfHOQxdE4FJo6anSPFBJL9Db49w5p_t9KDszDsWHGOvuaV-MUKJSEP-FIKkSgvMKX_8Ft2mf62GqYUwrgJaTqt4dlc-plBx6M-dhtHkxQMwhE1MzMUKbQyZVvzrN3LsxdA_2FEIFb07AFm9jn-3kh_LgJAXBQFcnjm6YDoHZXynHzuzsElP-00T_vcHLY2Nvk7F3ubrrby0hghDWEkF_A1NRrTM</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Maeda, M.(Okayama Univ. (Japan))</creator><creator>Kamamoto, M</creator><creator>Hino, K</creator><creator>Yamamoto, S</creator><creator>Kimura, M</creator><creator>Okano, M</creator><creator>Kimura, Y</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Glycoform analysis of Japanese cedar [Cryptomeria japonica] pollen allergen, Cry j 1</title><author>Maeda, M.(Okayama Univ. 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Psychology</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>HYPERSENSITIVITY</topic><topic>Japanese cedar pollen</topic><topic>Lewis a epitope</topic><topic>Molecular Sequence Data</topic><topic>N-glycan</topic><topic>Plant Proteins - analysis</topic><topic>Plant Proteins - chemistry</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>Pollen - chemistry</topic><topic>pollen allergen</topic><topic>Polysaccharides - chemistry</topic><topic>REACCIONES ALERGICAS</topic><topic>REACTION ALLERGIQUE</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>STRUCTURE CHIMIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maeda, M.(Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Kamamoto, M</creatorcontrib><creatorcontrib>Hino, K</creatorcontrib><creatorcontrib>Yamamoto, S</creatorcontrib><creatorcontrib>Kimura, M</creatorcontrib><creatorcontrib>Okano, M</creatorcontrib><creatorcontrib>Kimura, Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maeda, M.(Okayama Univ. (Japan))</au><au>Kamamoto, M</au><au>Hino, K</au><au>Yamamoto, S</au><au>Kimura, M</au><au>Okano, M</au><au>Kimura, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycoform analysis of Japanese cedar [Cryptomeria japonica] pollen allergen, Cry j 1</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2005</date><risdate>2005</risdate><volume>69</volume><issue>9</issue><spage>1700</spage><epage>1705</epage><pages>1700-1705</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137-144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exoand endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESIMS). Structural analysis clearly indicated that Lewis a epitope (Gal beta l-3(Fuc alpha l4) GlcNAc beta 1-), instead of the Gal beta l-4(Fuc alpha l-6) GlcNAc, occurs in the N-glycans of Cry j 1.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>16195587</pmid><doi>10.1271/bbb.69.1700</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | ALERGENOS ALLERGENE ALLERGENS Allergens - analysis Allergens - chemistry Antigens, Plant Biological and medical sciences Carbohydrate Sequence CHEMICAL STRUCTURE Cry j 1 Cryptomeria CRYPTOMERIA JAPONICA Epitopes - analysis Epitopes - chemistry ESTRUCTURA QUIMICA Fundamental and applied biological sciences. Psychology Glycoside Hydrolases - metabolism HYPERSENSITIVITY Japanese cedar pollen Lewis a epitope Molecular Sequence Data N-glycan Plant Proteins - analysis Plant Proteins - chemistry POLEN POLLEN Pollen - chemistry pollen allergen Polysaccharides - chemistry REACCIONES ALERGICAS REACTION ALLERGIQUE Spectrometry, Mass, Electrospray Ionization STRUCTURE CHIMIQUE |
| title | Glycoform analysis of Japanese cedar [Cryptomeria japonica] pollen allergen, Cry j 1 |
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