Cloning of the phloem-specific small heat-shock protein from leaves of rice plants
The N-terminal amino-acid sequence of a major rice phloem-sap protein, designated as RPP23, was determined. The complete amino-acid sequence of RPP23 was deduced from the corresponding rice EST-clone and contained an extra 46 amino acids at the N-terminus, that was apparently cleaved off to form mat...
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Veröffentlicht in: | Soil science and plant nutrition (Tokyo) 2004-12, Vol.50 (8), p.1255-1262 |
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creator | Fukuda, A. (Tokyo Univ. (Japan)) Okada, Y Suzui, N Fujiwara, T Yoneyama, T Hayashi, H |
description | The N-terminal amino-acid sequence of a major rice phloem-sap protein, designated as RPP23, was determined. The complete amino-acid sequence of RPP23 was deduced from the corresponding rice EST-clone and contained an extra 46 amino acids at the N-terminus, that was apparently cleaved off to form mature RPP23 in sieve tubes. RPP23 shared a similarity to plant small heat-shock proteins (smHSPs), though the N-terminal region of RPP23 was distinct from that of known smHSPs. Immunocytological analyses using leaf sections showed that RPP23 was located only in the phloem regions of leaves, and was present in non-stressed plants. In mature leaves, stronger immunocytological signals were detected in sieve elements than in companion cells. |
doi_str_mv | 10.1080/00380768.2004.10408601 |
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(Tokyo Univ. (Japan)) ; Okada, Y ; Suzui, N ; Fujiwara, T ; Yoneyama, T ; Hayashi, H</creator><creatorcontrib>Fukuda, A. (Tokyo Univ. (Japan)) ; Okada, Y ; Suzui, N ; Fujiwara, T ; Yoneyama, T ; Hayashi, H</creatorcontrib><description>The N-terminal amino-acid sequence of a major rice phloem-sap protein, designated as RPP23, was determined. The complete amino-acid sequence of RPP23 was deduced from the corresponding rice EST-clone and contained an extra 46 amino acids at the N-terminus, that was apparently cleaved off to form mature RPP23 in sieve tubes. RPP23 shared a similarity to plant small heat-shock proteins (smHSPs), though the N-terminal region of RPP23 was distinct from that of known smHSPs. Immunocytological analyses using leaf sections showed that RPP23 was located only in the phloem regions of leaves, and was present in non-stressed plants. In mature leaves, stronger immunocytological signals were detected in sieve elements than in companion cells.</description><identifier>ISSN: 0038-0768</identifier><identifier>EISSN: 1747-0765</identifier><identifier>DOI: 10.1080/00380768.2004.10408601</identifier><language>eng</language><publisher>Taylor & Francis Group</publisher><subject>HEAT SHOCK PROTEINS ; MOLECULAR CLONING ; ORYZA SATIVA ; PHLOEM ; rice ; sieve tube ; small heat-shock protein</subject><ispartof>Soil science and plant nutrition (Tokyo), 2004-12, Vol.50 (8), p.1255-1262</ispartof><rights>Copyright Taylor & Francis Group, LLC 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-f3165cb9c62310692e8b849f82100c55f531cb9696ec6b88b3269eb6cb99f5913</citedby><cites>FETCH-LOGICAL-c474t-f3165cb9c62310692e8b849f82100c55f531cb9696ec6b88b3269eb6cb99f5913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Fukuda, A. 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Immunocytological analyses using leaf sections showed that RPP23 was located only in the phloem regions of leaves, and was present in non-stressed plants. In mature leaves, stronger immunocytological signals were detected in sieve elements than in companion cells.</description><subject>HEAT SHOCK PROTEINS</subject><subject>MOLECULAR CLONING</subject><subject>ORYZA SATIVA</subject><subject>PHLOEM</subject><subject>rice</subject><subject>sieve tube</subject><subject>small heat-shock protein</subject><issn>0038-0768</issn><issn>1747-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkMtOxCAYhYnRxPHyCBpeoCOUS2E5mXjNJBqja0IRpigtDTSaeXtpxlm7-v-cc76zOABcY7TESKAbhIhADRfLGiFaJIoER_gILHBDm6o47Bgs5tD8i1NwlvNnSVLC6gV4XYc4-GELo4NTZ-HYhWj7Ko_WeOcNzL0OAXZWT1XuovmCY4qT9QN0KfYwWP1t88wmbwoc9DDlC3DidMj28u-eg_e727f1Q7V5vn9crzaVoQ2dKkcwZ6aVhtcEIy5rK1pBpRM1Rsgw5hjBxeaSW8NbIVpSc2lbXjTpmMTkHPB9r0kx52SdGpPvddopjNS8jDoso-Zl1GGZAq72oB9cTL3-iSl8qEnvQkwu6cH4rMi_HVf7Dqej0ttUkKeXEmElJRghvzRccv4</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Fukuda, A. 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(Japan))</creatorcontrib><creatorcontrib>Okada, Y</creatorcontrib><creatorcontrib>Suzui, N</creatorcontrib><creatorcontrib>Fujiwara, T</creatorcontrib><creatorcontrib>Yoneyama, T</creatorcontrib><creatorcontrib>Hayashi, H</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Soil science and plant nutrition (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukuda, A. (Tokyo Univ. 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RPP23 shared a similarity to plant small heat-shock proteins (smHSPs), though the N-terminal region of RPP23 was distinct from that of known smHSPs. Immunocytological analyses using leaf sections showed that RPP23 was located only in the phloem regions of leaves, and was present in non-stressed plants. In mature leaves, stronger immunocytological signals were detected in sieve elements than in companion cells.</abstract><pub>Taylor & Francis Group</pub><doi>10.1080/00380768.2004.10408601</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | HEAT SHOCK PROTEINS MOLECULAR CLONING ORYZA SATIVA PHLOEM rice sieve tube small heat-shock protein |
title | Cloning of the phloem-specific small heat-shock protein from leaves of rice plants |
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