Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase
We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a sma...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1353-1356 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Iwakiri, R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) Yoshihira, K Ngadiman Futagami, T Goto, M Furukawa, K |
| description | We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA. |
| doi_str_mv | 10.1271/bbb.68.1353 |
| format | Article |
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(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) ; Yoshihira, K ; Ngadiman ; Futagami, T ; Goto, M ; Furukawa, K</creator><creatorcontrib>Iwakiri, R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) ; Yoshihira, K ; Ngadiman ; Futagami, T ; Goto, M ; Furukawa, K</creatorcontrib><description>We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.68.1353</identifier><identifier>PMID: 15215602</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ALCALIGENES ; Alcaligenes - enzymology ; Alcaligenes - genetics ; Alcaligenes - metabolism ; BIODEGRADATION ; Biodegradation, Environmental ; Biological and medical sciences ; biphenyl dioxygenase ; CAMPHOR ; Camphor 5-Monooxygenase - genetics ; camphor monooxygenase ; chloroethanes ; Dioxygenases - genetics ; Ethane - analogs & derivatives ; Ethane - metabolism ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; Genetic Engineering ; Hydrocarbons, Chlorinated - metabolism ; LYASES ; ORGANOCHLORINE COMPOUNDS ; Pseudomonas putida ; TRANSGENICS</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1353-1356</ispartof><rights>2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-af904840b0cd7fd17d1db00a81590fb1c3088ed296c85fb0c55e2967882303b63</citedby><cites>FETCH-LOGICAL-c569t-af904840b0cd7fd17d1db00a81590fb1c3088ed296c85fb0c55e2967882303b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16056829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15215602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwakiri, R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Yoshihira, K</creatorcontrib><creatorcontrib>Ngadiman</creatorcontrib><creatorcontrib>Futagami, T</creatorcontrib><creatorcontrib>Goto, M</creatorcontrib><creatorcontrib>Furukawa, K</creatorcontrib><title>Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA.</description><subject>ALCALIGENES</subject><subject>Alcaligenes - enzymology</subject><subject>Alcaligenes - genetics</subject><subject>Alcaligenes - metabolism</subject><subject>BIODEGRADATION</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>biphenyl dioxygenase</subject><subject>CAMPHOR</subject><subject>Camphor 5-Monooxygenase - genetics</subject><subject>camphor monooxygenase</subject><subject>chloroethanes</subject><subject>Dioxygenases - genetics</subject><subject>Ethane - analogs & derivatives</subject><subject>Ethane - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Genetic Engineering</subject><subject>Hydrocarbons, Chlorinated - metabolism</subject><subject>LYASES</subject><subject>ORGANOCHLORINE COMPOUNDS</subject><subject>Pseudomonas putida</subject><subject>TRANSGENICS</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0U2L1DAYB_Agiju7evKsBEQv0jFpmjQ9LovrCwt6WM_haV46WdqkJh3cfgq_shlmdEE8heT5PQ9J_gi9oGRL65a-7_t-K-SWMs4eoQ1lTVuJrmkfow3pqKhkw-kZOs_5jpBywOlTdEZ5Tbkg9Qb9uo0LjNjYIYGBxceAo8OzDQvo3RhTtMsOgsX9iiFgGwYfrE3W4MtRw-gHG2zGeUngS_V-TjZnHwYMeIrGO1-ghmnexVQOQoz3a-mAbMswU9Bu7ZM32Pi_hWfoiYMx2-en9QJ9v_5we_Wpuvn68fPV5U2lueiWClxHGtmQnmjTOkNbQ01PCEjKO-J6qhmR0pq6E1pyVxTntmxaKWtGWC_YBXp7nDun-GNv86Imn7Udx_LYuM-Ktm3LJGUFvv4H3sV9CuVuijZNJ0nbsIN6d1Q6xZyTdWpOfoK0KkrUISVVUlJCqkNKRb86zdz3kzUP9hRLAW9OAHL5ZpcgaJ8fnCBcyLorThydDy6mCX7GNBq1wFqS-9PE_n-Dl8dGB1HBkIr78q0mhBNC61L_DUBqt5I</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Iwakiri, R. 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Faculty of Agriculture) ; Yoshihira, K ; Ngadiman ; Futagami, T ; Goto, M ; Furukawa, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-af904840b0cd7fd17d1db00a81590fb1c3088ed296c85fb0c55e2967882303b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>ALCALIGENES</topic><topic>Alcaligenes - enzymology</topic><topic>Alcaligenes - genetics</topic><topic>Alcaligenes - metabolism</topic><topic>BIODEGRADATION</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>biphenyl dioxygenase</topic><topic>CAMPHOR</topic><topic>Camphor 5-Monooxygenase - genetics</topic><topic>camphor monooxygenase</topic><topic>chloroethanes</topic><topic>Dioxygenases - genetics</topic><topic>Ethane - analogs & derivatives</topic><topic>Ethane - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Genetic Engineering</topic><topic>Hydrocarbons, Chlorinated - metabolism</topic><topic>LYASES</topic><topic>ORGANOCHLORINE COMPOUNDS</topic><topic>Pseudomonas putida</topic><topic>TRANSGENICS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwakiri, R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Yoshihira, K</creatorcontrib><creatorcontrib>Ngadiman</creatorcontrib><creatorcontrib>Futagami, T</creatorcontrib><creatorcontrib>Goto, M</creatorcontrib><creatorcontrib>Furukawa, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwakiri, R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture)</au><au>Yoshihira, K</au><au>Ngadiman</au><au>Futagami, T</au><au>Goto, M</au><au>Furukawa, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>68</volume><issue>6</issue><spage>1353</spage><epage>1356</epage><pages>1353-1356</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15215602</pmid><doi>10.1271/bbb.68.1353</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2004-06, Vol.68 (6), p.1353-1356 |
| issn | 0916-8451 1347-6947 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | ALCALIGENES Alcaligenes - enzymology Alcaligenes - genetics Alcaligenes - metabolism BIODEGRADATION Biodegradation, Environmental Biological and medical sciences biphenyl dioxygenase CAMPHOR Camphor 5-Monooxygenase - genetics camphor monooxygenase chloroethanes Dioxygenases - genetics Ethane - analogs & derivatives Ethane - metabolism Fundamental and applied biological sciences. Psychology GENE EXPRESSION Genetic Engineering Hydrocarbons, Chlorinated - metabolism LYASES ORGANOCHLORINE COMPOUNDS Pseudomonas putida TRANSGENICS |
| title | Total degradation of pentachloroethane by an engineered Alcaligenes strain expressing a modified camphor monooxygenase and a hybrid dioxygenase |
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