Structural analysis of TRG1@ and TRG2@ loci of the ovine immune system

[By isolating sheep genomic clones related to constant genes of T cell receptor gamma chain, we have shown, by FISH on sheep choromosomes, the occurrence of parologous loci TRG1@ and TRG2@ (Massari, 1998). They are separated by at least five chromosome bands on chromosome 4: locus TRG1@ maps in 4q31...

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Veröffentlicht in:Italian journal of animal science 2003, Vol.2, p.49-51
Hauptverfasser: Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica), Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica), Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica), Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica), Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale), Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)
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container_title Italian journal of animal science
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creator Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)
Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)
Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)
Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)
Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale)
Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)
description [By isolating sheep genomic clones related to constant genes of T cell receptor gamma chain, we have shown, by FISH on sheep choromosomes, the occurrence of parologous loci TRG1@ and TRG2@ (Massari, 1998). They are separated by at least five chromosome bands on chromosome 4: locus TRG1@ maps in 4q31, in a region homologous with human TRG locus on chromosome 7, while locus TRG2@ maps on 4q15-22 and is characteristic of ruminants. In this work, the whole structure of loci TRG1@ and TRG2@, derived by the analysis of six sheep BAC clones, is reported. TRG2@ has a total length of 90 Kpb and is composed by three clusters (TRG6, TRG2 and TRG4). TRG1 is 150 Kbp long and is composed by TRG5, TRG3 and TRG1 clusters.] Grazie all´isolamento di cloni genomici ovini relativi ai geni costanti della catena gamma del T cell receptor, abbiamo dimostrato, mediante FISH su cromosomi di ovino, la presenza dei loci paraloghi TRG1@ e TRG2@ (Massari, 1998). Essi risultano separati da almeno cinque bande cromosomiche sul cromosoma 4: il locus TRG1@ mappa in 4q31, in una regione di omologia con il locus TRG umano sul cromosoma 7, mentre il locus TRG2@ mappa in 4q15-22 ed è caratteristico dei ruminanti. E´ qui riportata l´intera struttura dei loci TRG1@ e TRG2@, dedotta attraverso l´analisi di sei cloni BAC ovini. TRG2@ si estende per circa 90 Kpb e consiste di tre cluster (TRG6, TRG2 e TRG4). TRG1@, invece, è lungo circa 150 Kpb ed è composto dai cluster TRG5, TRG3 e TRG1
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(Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale) ; Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</creator><creatorcontrib>Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale) ; Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</creatorcontrib><description>[By isolating sheep genomic clones related to constant genes of T cell receptor gamma chain, we have shown, by FISH on sheep choromosomes, the occurrence of parologous loci TRG1@ and TRG2@ (Massari, 1998). They are separated by at least five chromosome bands on chromosome 4: locus TRG1@ maps in 4q31, in a region homologous with human TRG locus on chromosome 7, while locus TRG2@ maps on 4q15-22 and is characteristic of ruminants. In this work, the whole structure of loci TRG1@ and TRG2@, derived by the analysis of six sheep BAC clones, is reported. TRG2@ has a total length of 90 Kpb and is composed by three clusters (TRG6, TRG2 and TRG4). TRG1 is 150 Kbp long and is composed by TRG5, TRG3 and TRG1 clusters.] Grazie all´isolamento di cloni genomici ovini relativi ai geni costanti della catena gamma del T cell receptor, abbiamo dimostrato, mediante FISH su cromosomi di ovino, la presenza dei loci paraloghi TRG1@ e TRG2@ (Massari, 1998). Essi risultano separati da almeno cinque bande cromosomiche sul cromosoma 4: il locus TRG1@ mappa in 4q31, in una regione di omologia con il locus TRG umano sul cromosoma 7, mentre il locus TRG2@ mappa in 4q15-22 ed è caratteristico dei ruminanti. E´ qui riportata l´intera struttura dei loci TRG1@ e TRG2@, dedotta attraverso l´analisi di sei cloni BAC ovini. TRG2@ si estende per circa 90 Kpb e consiste di tre cluster (TRG6, TRG2 e TRG4). TRG1@, invece, è lungo circa 150 Kpb ed è composto dai cluster TRG5, TRG3 e TRG1</description><identifier>ISSN: 1594-4077</identifier><identifier>EISSN: 1828-051X</identifier><identifier>DOI: 10.4081/ijas.2003.11675911</identifier><language>eng</language><publisher>Taylor &amp; Francis</publisher><subject>banche di geni ; BANCO DE GENES ; BANQUE DE GENES ; CHROMOSOME ; chromosomes ; CLONE ; clones ; cloni ; CROMOSOMAS ; cromosomi ; GENE ; gene banks ; genes ; genetic variation ; geni ; genoma ; GENOMAS ; GENOME ; genomes ; IMMUNITE ; immunity ; immunità ; INMUNIDAD ; linfociti ; LINFOCITOS ; loci ; LOCUS ; locus structural genomic organization ; LYMPHOCYTE ; lymphocytes ; nucleotide sequence ; OVIN ; ovini ; OVINOS ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; sequenza nucleotidica ; sheep ; T cell receptor ; TRG@ loci ; VARIACION GENETICA ; VARIATION GENETIQUE ; variazione genetica</subject><ispartof>Italian journal of animal science, 2003, Vol.2, p.49-51</ispartof><rights>2003 Taylor &amp; Francis Group LLC 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale)</creatorcontrib><creatorcontrib>Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</creatorcontrib><title>Structural analysis of TRG1@ and TRG2@ loci of the ovine immune system</title><title>Italian journal of animal science</title><description>[By isolating sheep genomic clones related to constant genes of T cell receptor gamma chain, we have shown, by FISH on sheep choromosomes, the occurrence of parologous loci TRG1@ and TRG2@ (Massari, 1998). They are separated by at least five chromosome bands on chromosome 4: locus TRG1@ maps in 4q31, in a region homologous with human TRG locus on chromosome 7, while locus TRG2@ maps on 4q15-22 and is characteristic of ruminants. In this work, the whole structure of loci TRG1@ and TRG2@, derived by the analysis of six sheep BAC clones, is reported. TRG2@ has a total length of 90 Kpb and is composed by three clusters (TRG6, TRG2 and TRG4). TRG1 is 150 Kbp long and is composed by TRG5, TRG3 and TRG1 clusters.] Grazie all´isolamento di cloni genomici ovini relativi ai geni costanti della catena gamma del T cell receptor, abbiamo dimostrato, mediante FISH su cromosomi di ovino, la presenza dei loci paraloghi TRG1@ e TRG2@ (Massari, 1998). Essi risultano separati da almeno cinque bande cromosomiche sul cromosoma 4: il locus TRG1@ mappa in 4q31, in una regione di omologia con il locus TRG umano sul cromosoma 7, mentre il locus TRG2@ mappa in 4q15-22 ed è caratteristico dei ruminanti. E´ qui riportata l´intera struttura dei loci TRG1@ e TRG2@, dedotta attraverso l´analisi di sei cloni BAC ovini. TRG2@ si estende per circa 90 Kpb e consiste di tre cluster (TRG6, TRG2 e TRG4). TRG1@, invece, è lungo circa 150 Kpb ed è composto dai cluster TRG5, TRG3 e TRG1</description><subject>banche di geni</subject><subject>BANCO DE GENES</subject><subject>BANQUE DE GENES</subject><subject>CHROMOSOME</subject><subject>chromosomes</subject><subject>CLONE</subject><subject>clones</subject><subject>cloni</subject><subject>CROMOSOMAS</subject><subject>cromosomi</subject><subject>GENE</subject><subject>gene banks</subject><subject>genes</subject><subject>genetic variation</subject><subject>geni</subject><subject>genoma</subject><subject>GENOMAS</subject><subject>GENOME</subject><subject>genomes</subject><subject>IMMUNITE</subject><subject>immunity</subject><subject>immunità</subject><subject>INMUNIDAD</subject><subject>linfociti</subject><subject>LINFOCITOS</subject><subject>loci</subject><subject>LOCUS</subject><subject>locus structural genomic organization</subject><subject>LYMPHOCYTE</subject><subject>lymphocytes</subject><subject>nucleotide sequence</subject><subject>OVIN</subject><subject>ovini</subject><subject>OVINOS</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>sequenza nucleotidica</subject><subject>sheep</subject><subject>T cell receptor</subject><subject>TRG@ loci</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><subject>variazione genetica</subject><issn>1594-4077</issn><issn>1828-051X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><recordid>eNo1kNFKwzAYhYMoOKcvIAh9gc7_T5omuxAmw83BQNAJ3oU0S7SjbSBplb29KdOrcziccy4-Qm4RZgVIvK8POs4oAJshloLPEc_IBCWVOXD8OE-ez4u8ACEuyVWMB4ASGGUTsnrrw2D6Iegm051ujrGOmXfZ7nWNi5TsR0cXWeNNPeb9l838d93ZrG7bIUk8xt621-TC6Sbamz-dkvfV0275nG9f1pvl4zZ3WJR9XlnKqawKJxyMnnM0FCuktrLSiH0lJLUWqGFujgAFL6WT3AnqpAFpHJuSh9Nv3TkfWv3jQ7NXvT42PrigO1NHxRDUCEWNUNQIRf1DSfu7095pr_RnSPXNLlV4CakC7BcpaV6J</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creator><creator>Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creator><creator>Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creator><creator>Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creator><creator>Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale)</creator><creator>Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</creator><general>Taylor &amp; Francis</general><scope>FBQ</scope><scope>0YH</scope></search><sort><creationdate>2003</creationdate><title>Structural analysis of TRG1@ and TRG2@ loci of the ovine immune system</title><author>Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica) ; Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale) ; Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). 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(Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</creatorcontrib><creatorcontrib>Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale)</creatorcontrib><creatorcontrib>Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</creatorcontrib><collection>AGRIS</collection><collection>Taylor &amp; Francis Open Access</collection><jtitle>Italian journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaccarelli, G. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</au><au>Miccoli, M.C. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</au><au>Giovinazzo, M. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</au><au>Ciccarese, S. (Bari Univ. (Italy). Dipartimento di Anatomia Patologica e Genetica)</au><au>Caputi Jambrenghi, A. (Bari Univ. (Italy). Dipartimento di Produzione Animale)</au><au>Cribiu, E.P. (Institut National de la Recherche Agronomique (INRA), Jouy en Josas (France). Dept. de Genetique Animale)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural analysis of TRG1@ and TRG2@ loci of the ovine immune system</atitle><jtitle>Italian journal of animal science</jtitle><date>2003</date><risdate>2003</risdate><volume>2</volume><spage>49</spage><epage>51</epage><pages>49-51</pages><issn>1594-4077</issn><eissn>1828-051X</eissn><abstract>[By isolating sheep genomic clones related to constant genes of T cell receptor gamma chain, we have shown, by FISH on sheep choromosomes, the occurrence of parologous loci TRG1@ and TRG2@ (Massari, 1998). They are separated by at least five chromosome bands on chromosome 4: locus TRG1@ maps in 4q31, in a region homologous with human TRG locus on chromosome 7, while locus TRG2@ maps on 4q15-22 and is characteristic of ruminants. In this work, the whole structure of loci TRG1@ and TRG2@, derived by the analysis of six sheep BAC clones, is reported. TRG2@ has a total length of 90 Kpb and is composed by three clusters (TRG6, TRG2 and TRG4). TRG1 is 150 Kbp long and is composed by TRG5, TRG3 and TRG1 clusters.] Grazie all´isolamento di cloni genomici ovini relativi ai geni costanti della catena gamma del T cell receptor, abbiamo dimostrato, mediante FISH su cromosomi di ovino, la presenza dei loci paraloghi TRG1@ e TRG2@ (Massari, 1998). Essi risultano separati da almeno cinque bande cromosomiche sul cromosoma 4: il locus TRG1@ mappa in 4q31, in una regione di omologia con il locus TRG umano sul cromosoma 7, mentre il locus TRG2@ mappa in 4q15-22 ed è caratteristico dei ruminanti. E´ qui riportata l´intera struttura dei loci TRG1@ e TRG2@, dedotta attraverso l´analisi di sei cloni BAC ovini. TRG2@ si estende per circa 90 Kpb e consiste di tre cluster (TRG6, TRG2 e TRG4). TRG1@, invece, è lungo circa 150 Kpb ed è composto dai cluster TRG5, TRG3 e TRG1</abstract><pub>Taylor &amp; Francis</pub><doi>10.4081/ijas.2003.11675911</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record>
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subjects banche di geni
BANCO DE GENES
BANQUE DE GENES
CHROMOSOME
chromosomes
CLONE
clones
cloni
CROMOSOMAS
cromosomi
GENE
gene banks
genes
genetic variation
geni
genoma
GENOMAS
GENOME
genomes
IMMUNITE
immunity
immunità
INMUNIDAD
linfociti
LINFOCITOS
loci
LOCUS
locus structural genomic organization
LYMPHOCYTE
lymphocytes
nucleotide sequence
OVIN
ovini
OVINOS
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
sequenza nucleotidica
sheep
T cell receptor
TRG@ loci
VARIACION GENETICA
VARIATION GENETIQUE
variazione genetica
title Structural analysis of TRG1@ and TRG2@ loci of the ovine immune system
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