An analysis of seed development in Pisum sativum L. 16. Assessing variation for fatty-acid content by use of a non-destructive technique for single-seed analysis
Analyses of seeds from a wide range of pea lines have revealed extensive variation for all of the major fatty acids normally found in pisum. Unlike total lipid content, this variation was not affected by the presence of mutant alleles at the r and rb loci. To allow chemically analysed seed to be use...
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| Veröffentlicht in: | Plant breeding 1995, Vol.114 (1) |
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| container_title | Plant breeding |
| container_volume | 114 |
| creator | Jones, D.A Barber, L.M Arthur, A.E Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics) |
| description | Analyses of seeds from a wide range of pea lines have revealed extensive variation for all of the major fatty acids normally found in pisum. Unlike total lipid content, this variation was not affected by the presence of mutant alleles at the r and rb loci. To allow chemically analysed seed to be used in crosses, which is of particular importance for F2 seed, a method has been developed by which individual seeds can be analysed non-destructively. This system entails drilling a small hole in the seed, away from the embryonic axis. The small quantity of powder produced by this process can be used for the chemical analysis but does not prevent the seed from being grown to produce progeny. Reducing the sample size had no significant effect upon the compositional analysis of the fatty acids. |
| format | Article |
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Assessing variation for fatty-acid content by use of a non-destructive technique for single-seed analysis</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Jones, D.A ; Barber, L.M ; Arthur, A.E ; Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics)</creator><creatorcontrib>Jones, D.A ; Barber, L.M ; Arthur, A.E ; Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics)</creatorcontrib><description>Analyses of seeds from a wide range of pea lines have revealed extensive variation for all of the major fatty acids normally found in pisum. Unlike total lipid content, this variation was not affected by the presence of mutant alleles at the r and rb loci. To allow chemically analysed seed to be used in crosses, which is of particular importance for F2 seed, a method has been developed by which individual seeds can be analysed non-destructively. This system entails drilling a small hole in the seed, away from the embryonic axis. The small quantity of powder produced by this process can be used for the chemical analysis but does not prevent the seed from being grown to produce progeny. Reducing the sample size had no significant effect upon the compositional analysis of the fatty acids.</description><identifier>ISSN: 0179-9541</identifier><identifier>EISSN: 1439-0523</identifier><language>eng</language><subject>ACIDE GRAS ; ACIDOS GRASOS ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; fatty-acid composition ; Gartenbau ; GENE ; GENES ; MUTANT ; MUTANTES ; Pflanzenzuechtung ; PISUM SATIVUM ; single-seed analysis ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; VARIACION GENETICA ; variation ; VARIATION GENETIQUE</subject><ispartof>Plant breeding, 1995, Vol.114 (1)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Jones, D.A</creatorcontrib><creatorcontrib>Barber, L.M</creatorcontrib><creatorcontrib>Arthur, A.E</creatorcontrib><creatorcontrib>Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics)</creatorcontrib><title>An analysis of seed development in Pisum sativum L. 16. Assessing variation for fatty-acid content by use of a non-destructive technique for single-seed analysis</title><title>Plant breeding</title><description>Analyses of seeds from a wide range of pea lines have revealed extensive variation for all of the major fatty acids normally found in pisum. Unlike total lipid content, this variation was not affected by the presence of mutant alleles at the r and rb loci. To allow chemically analysed seed to be used in crosses, which is of particular importance for F2 seed, a method has been developed by which individual seeds can be analysed non-destructively. This system entails drilling a small hole in the seed, away from the embryonic axis. The small quantity of powder produced by this process can be used for the chemical analysis but does not prevent the seed from being grown to produce progeny. Reducing the sample size had no significant effect upon the compositional analysis of the fatty acids.</description><subject>ACIDE GRAS</subject><subject>ACIDOS GRASOS</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>fatty-acid composition</subject><subject>Gartenbau</subject><subject>GENE</subject><subject>GENES</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Pflanzenzuechtung</subject><subject>PISUM SATIVUM</subject><subject>single-seed analysis</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>VARIACION GENETICA</subject><subject>variation</subject><subject>VARIATION GENETIQUE</subject><issn>0179-9541</issn><issn>1439-0523</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9jjFOw0AQRVcIpJiEC6SaC2xkZxNbLgMEUVBQ0EcTezYMcmbBs7bk43BT7Ahaqqevr__0r0ySbVxp0-3aXZskzYrSlttNNjO3qh_plF2RmO-dAAo2g7JC8KBENdTUUxM-zyQRWOCVtTuDYuR-5MsKsnwFO1VSZTlBjy2PXRDwoQWPMQ4WK66hChInxXGATmmyI0gQW5PGtqtGHUGk6l34q6PLePI1ZC8n_l4tzI3HRunul3OzfNq_PTxbj-GAp5b18Lgv8_sid879W_4AbFlWZQ</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Jones, D.A</creator><creator>Barber, L.M</creator><creator>Arthur, A.E</creator><creator>Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics)</creator><scope>FBQ</scope></search><sort><creationdate>1995</creationdate><title>An analysis of seed development in Pisum sativum L. 16. Assessing variation for fatty-acid content by use of a non-destructive technique for single-seed analysis</title><author>Jones, D.A ; Barber, L.M ; Arthur, A.E ; Hedley, C.L. (John Innes Centre, Norwich, Norfolk (United Kingdom). Dept. of Applied Genetics)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_DE96B76333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ACIDE GRAS</topic><topic>ACIDOS GRASOS</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>fatty-acid composition</topic><topic>Gartenbau</topic><topic>GENE</topic><topic>GENES</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Pflanzenzuechtung</topic><topic>PISUM SATIVUM</topic><topic>single-seed analysis</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>VARIACION GENETICA</topic><topic>variation</topic><topic>VARIATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, D.A</creatorcontrib><creatorcontrib>Barber, L.M</creatorcontrib><creatorcontrib>Arthur, A.E</creatorcontrib><creatorcontrib>Hedley, C.L. 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Unlike total lipid content, this variation was not affected by the presence of mutant alleles at the r and rb loci. To allow chemically analysed seed to be used in crosses, which is of particular importance for F2 seed, a method has been developed by which individual seeds can be analysed non-destructively. This system entails drilling a small hole in the seed, away from the embryonic axis. The small quantity of powder produced by this process can be used for the chemical analysis but does not prevent the seed from being grown to produce progeny. Reducing the sample size had no significant effect upon the compositional analysis of the fatty acids.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0179-9541 |
| ispartof | Plant breeding, 1995, Vol.114 (1) |
| issn | 0179-9541 1439-0523 |
| language | eng |
| recordid | cdi_fao_agris_DE96B7633 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | ACIDE GRAS ACIDOS GRASOS COMPOSICION QUIMICA COMPOSITION CHIMIQUE fatty-acid composition Gartenbau GENE GENES MUTANT MUTANTES Pflanzenzuechtung PISUM SATIVUM single-seed analysis TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS VARIACION GENETICA variation VARIATION GENETIQUE |
| title | An analysis of seed development in Pisum sativum L. 16. Assessing variation for fatty-acid content by use of a non-destructive technique for single-seed analysis |
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