Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta
Ravenelia esculenta is a rust fungus which infects Acacia eburnea producing hypertrophy in the infected organs. We analysed the changes in the reaction patterns of peroxidase from the tissues at progressive disease stages. Peroxidases (POX) from the tissues were analysed for oxidation of reduced nic...
Gespeichert in:
| Veröffentlicht in: | Plant, soil and environment soil and environment, 2010-01, Vol.56 (9) |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | |
| container_title | Plant, soil and environment |
| container_volume | 56 |
| creator | Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany |
| description | Ravenelia esculenta is a rust fungus which infects Acacia eburnea producing hypertrophy in the infected organs. We analysed the changes in the reaction patterns of peroxidase from the tissues at progressive disease stages. Peroxidases (POX) from the tissues were analysed for oxidation of reduced nicotinamine adenine dinucleotide (NADH), NADH-dependent hydrogen peroxide (H2O2) generation and polymerization of cinnamyl alcohol, a lignin precursor in plants. The effect of cofactors like Mn2+ and dichlorophenol (DCP) was also studied. Effect of externally added H2O2 on NADH oxidation and cinnamyl alcohol polymerization was analyzed. NADH oxidation and H2O2 generation are accelerated by supplement of cofactors. The cofactor requirements of the two reactions are different. The amount of H2O2 generated varies with disease stages, but the rate of NADH oxidation is comparable. Externally added H2O2 has an accelerating effect on NADH oxidation. Cinnamyl alcohol polymerizing activity of POX increased with disease progression and was strongly inhibited by H2O2. Stimulation of these reactions by cofactors has a physiological significance in terms of susceptibility of the host. |
| doi_str_mv | 10.17221/120/2009-PSE |
| format | Article |
| fullrecord | <record><control><sourceid>fao</sourceid><recordid>TN_cdi_fao_agris_CZ2010000779</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CZ2010000779</sourcerecordid><originalsourceid>FETCH-LOGICAL-f177t-f83624c6599098747125eaf44d8ca9b1ef63fad05aef9a5148a8e276f204fbf13</originalsourceid><addsrcrecordid>eNo1T01LAzEUzEHBUnv0KOQPrM3LZjfZYynVCgXFj4uX8jb7Xo2sWdls1f77tlTnMswHAyPEFagbsFrDFLSaaqWq7PF5cSZGoMFkANZdiElKH-qAPAdn9Eik5a7puw1F-UV99xsakgdBPQ6hixJjI9uwiYGDPzn17r-IiZLkvvuUM48-oKR620dCGSKTH6iRP2F4l0_4fdhrj3ny25bigJfinLFNNPnjsXi9XbzMl9nq4e5-PltlDNYOGbu81MaXRVWpylljQReEbEzjPFY1EJc5Y6MKJK6wAOPQkbYla2W4ZsjH4vq0y9itcdOHtJ6_aQXH_9ZW-R6dEloe</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta</title><source>DOAJ Directory of Open Access Journals</source><creator>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany ; Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creator><creatorcontrib>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany ; Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creatorcontrib><description>Ravenelia esculenta is a rust fungus which infects Acacia eburnea producing hypertrophy in the infected organs. We analysed the changes in the reaction patterns of peroxidase from the tissues at progressive disease stages. Peroxidases (POX) from the tissues were analysed for oxidation of reduced nicotinamine adenine dinucleotide (NADH), NADH-dependent hydrogen peroxide (H2O2) generation and polymerization of cinnamyl alcohol, a lignin precursor in plants. The effect of cofactors like Mn2+ and dichlorophenol (DCP) was also studied. Effect of externally added H2O2 on NADH oxidation and cinnamyl alcohol polymerization was analyzed. NADH oxidation and H2O2 generation are accelerated by supplement of cofactors. The cofactor requirements of the two reactions are different. The amount of H2O2 generated varies with disease stages, but the rate of NADH oxidation is comparable. Externally added H2O2 has an accelerating effect on NADH oxidation. Cinnamyl alcohol polymerizing activity of POX increased with disease progression and was strongly inhibited by H2O2. Stimulation of these reactions by cofactors has a physiological significance in terms of susceptibility of the host.</description><identifier>ISSN: 1214-1178</identifier><identifier>DOI: 10.17221/120/2009-PSE</identifier><language>eng</language><subject>ACACIA ; ACACIA EBURNEA ; BIOCHEMICAL PATHWAYS ; BIOCHEMICAL REACTIONS ; CHAMPIGNON ; ENFERMEDADES FUNGOSAS ; FUNGAL DISEASES ; FUNGI ; HONGOS ; http://www.fao.org/aos/agrovoc#c_11042 ; http://www.fao.org/aos/agrovoc#c_13251 ; http://www.fao.org/aos/agrovoc#c_25446 ; http://www.fao.org/aos/agrovoc#c_27462 ; http://www.fao.org/aos/agrovoc#c_3145 ; http://www.fao.org/aos/agrovoc#c_32 ; http://www.fao.org/aos/agrovoc#c_3725 ; http://www.fao.org/aos/agrovoc#c_4328 ; http://www.fao.org/aos/agrovoc#c_6710 ; http://www.fao.org/aos/agrovoc#c_909 ; HYDROGEN PEROXIDE ; LIGNIFICACION ; LIGNIFICATION ; MALADIE FONGIQUE ; PEROXIDASAS ; PEROXIDASES ; PEROXIDO DE HIDROGENO ; PEROXYDASE ; PEROXYDE D'HYDROGENE ; PLANT RESPONSE ; RAVENELIA ESCULENTA ; REACCIONES BIOQUIMICAS ; REACTION BIOCHIMIQUE ; REPONSE DE LA PLANTE ; RESPUESTA DE LA PLANTA ; ROUILLE ; ROYA ; RUSTS ; VIA BIOQUIMICA DEL METABOLISMO ; VOIE BIOCHIMIQUE DU METABOLISME</subject><ispartof>Plant, soil and environment, 2010-01, Vol.56 (9)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creatorcontrib><creatorcontrib>Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creatorcontrib><title>Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta</title><title>Plant, soil and environment</title><description>Ravenelia esculenta is a rust fungus which infects Acacia eburnea producing hypertrophy in the infected organs. We analysed the changes in the reaction patterns of peroxidase from the tissues at progressive disease stages. Peroxidases (POX) from the tissues were analysed for oxidation of reduced nicotinamine adenine dinucleotide (NADH), NADH-dependent hydrogen peroxide (H2O2) generation and polymerization of cinnamyl alcohol, a lignin precursor in plants. The effect of cofactors like Mn2+ and dichlorophenol (DCP) was also studied. Effect of externally added H2O2 on NADH oxidation and cinnamyl alcohol polymerization was analyzed. NADH oxidation and H2O2 generation are accelerated by supplement of cofactors. The cofactor requirements of the two reactions are different. The amount of H2O2 generated varies with disease stages, but the rate of NADH oxidation is comparable. Externally added H2O2 has an accelerating effect on NADH oxidation. Cinnamyl alcohol polymerizing activity of POX increased with disease progression and was strongly inhibited by H2O2. Stimulation of these reactions by cofactors has a physiological significance in terms of susceptibility of the host.</description><subject>ACACIA</subject><subject>ACACIA EBURNEA</subject><subject>BIOCHEMICAL PATHWAYS</subject><subject>BIOCHEMICAL REACTIONS</subject><subject>CHAMPIGNON</subject><subject>ENFERMEDADES FUNGOSAS</subject><subject>FUNGAL DISEASES</subject><subject>FUNGI</subject><subject>HONGOS</subject><subject>http://www.fao.org/aos/agrovoc#c_11042</subject><subject>http://www.fao.org/aos/agrovoc#c_13251</subject><subject>http://www.fao.org/aos/agrovoc#c_25446</subject><subject>http://www.fao.org/aos/agrovoc#c_27462</subject><subject>http://www.fao.org/aos/agrovoc#c_3145</subject><subject>http://www.fao.org/aos/agrovoc#c_32</subject><subject>http://www.fao.org/aos/agrovoc#c_3725</subject><subject>http://www.fao.org/aos/agrovoc#c_4328</subject><subject>http://www.fao.org/aos/agrovoc#c_6710</subject><subject>http://www.fao.org/aos/agrovoc#c_909</subject><subject>HYDROGEN PEROXIDE</subject><subject>LIGNIFICACION</subject><subject>LIGNIFICATION</subject><subject>MALADIE FONGIQUE</subject><subject>PEROXIDASAS</subject><subject>PEROXIDASES</subject><subject>PEROXIDO DE HIDROGENO</subject><subject>PEROXYDASE</subject><subject>PEROXYDE D'HYDROGENE</subject><subject>PLANT RESPONSE</subject><subject>RAVENELIA ESCULENTA</subject><subject>REACCIONES BIOQUIMICAS</subject><subject>REACTION BIOCHIMIQUE</subject><subject>REPONSE DE LA PLANTE</subject><subject>RESPUESTA DE LA PLANTA</subject><subject>ROUILLE</subject><subject>ROYA</subject><subject>RUSTS</subject><subject>VIA BIOQUIMICA DEL METABOLISMO</subject><subject>VOIE BIOCHIMIQUE DU METABOLISME</subject><issn>1214-1178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNo1T01LAzEUzEHBUnv0KOQPrM3LZjfZYynVCgXFj4uX8jb7Xo2sWdls1f77tlTnMswHAyPEFagbsFrDFLSaaqWq7PF5cSZGoMFkANZdiElKH-qAPAdn9Eik5a7puw1F-UV99xsakgdBPQ6hixJjI9uwiYGDPzn17r-IiZLkvvuUM48-oKR620dCGSKTH6iRP2F4l0_4fdhrj3ny25bigJfinLFNNPnjsXi9XbzMl9nq4e5-PltlDNYOGbu81MaXRVWpylljQReEbEzjPFY1EJc5Y6MKJK6wAOPQkbYla2W4ZsjH4vq0y9itcdOHtJ6_aQXH_9ZW-R6dEloe</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creator><creator>Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creator><scope>FBQ</scope></search><sort><creationdate>20100101</creationdate><title>Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta</title><author>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany ; Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f177t-f83624c6599098747125eaf44d8ca9b1ef63fad05aef9a5148a8e276f204fbf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>ACACIA</topic><topic>ACACIA EBURNEA</topic><topic>BIOCHEMICAL PATHWAYS</topic><topic>BIOCHEMICAL REACTIONS</topic><topic>CHAMPIGNON</topic><topic>ENFERMEDADES FUNGOSAS</topic><topic>FUNGAL DISEASES</topic><topic>FUNGI</topic><topic>HONGOS</topic><topic>http://www.fao.org/aos/agrovoc#c_11042</topic><topic>http://www.fao.org/aos/agrovoc#c_13251</topic><topic>http://www.fao.org/aos/agrovoc#c_25446</topic><topic>http://www.fao.org/aos/agrovoc#c_27462</topic><topic>http://www.fao.org/aos/agrovoc#c_3145</topic><topic>http://www.fao.org/aos/agrovoc#c_32</topic><topic>http://www.fao.org/aos/agrovoc#c_3725</topic><topic>http://www.fao.org/aos/agrovoc#c_4328</topic><topic>http://www.fao.org/aos/agrovoc#c_6710</topic><topic>http://www.fao.org/aos/agrovoc#c_909</topic><topic>HYDROGEN PEROXIDE</topic><topic>LIGNIFICACION</topic><topic>LIGNIFICATION</topic><topic>MALADIE FONGIQUE</topic><topic>PEROXIDASAS</topic><topic>PEROXIDASES</topic><topic>PEROXIDO DE HIDROGENO</topic><topic>PEROXYDASE</topic><topic>PEROXYDE D'HYDROGENE</topic><topic>PLANT RESPONSE</topic><topic>RAVENELIA ESCULENTA</topic><topic>REACCIONES BIOQUIMICAS</topic><topic>REACTION BIOCHIMIQUE</topic><topic>REPONSE DE LA PLANTE</topic><topic>RESPUESTA DE LA PLANTA</topic><topic>ROUILLE</topic><topic>ROYA</topic><topic>RUSTS</topic><topic>VIA BIOQUIMICA DEL METABOLISMO</topic><topic>VOIE BIOCHIMIQUE DU METABOLISME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creatorcontrib><creatorcontrib>Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</creatorcontrib><collection>AGRIS</collection><jtitle>Plant, soil and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuvalekar, A.A.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</au><au>Gandhe, K.R.,Modern Coll. of Arts, Science and Commerce, Shivajinagar (India). Dept. of Botany</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta</atitle><jtitle>Plant, soil and environment</jtitle><date>2010-01-01</date><risdate>2010</risdate><volume>56</volume><issue>9</issue><issn>1214-1178</issn><abstract>Ravenelia esculenta is a rust fungus which infects Acacia eburnea producing hypertrophy in the infected organs. We analysed the changes in the reaction patterns of peroxidase from the tissues at progressive disease stages. Peroxidases (POX) from the tissues were analysed for oxidation of reduced nicotinamine adenine dinucleotide (NADH), NADH-dependent hydrogen peroxide (H2O2) generation and polymerization of cinnamyl alcohol, a lignin precursor in plants. The effect of cofactors like Mn2+ and dichlorophenol (DCP) was also studied. Effect of externally added H2O2 on NADH oxidation and cinnamyl alcohol polymerization was analyzed. NADH oxidation and H2O2 generation are accelerated by supplement of cofactors. The cofactor requirements of the two reactions are different. The amount of H2O2 generated varies with disease stages, but the rate of NADH oxidation is comparable. Externally added H2O2 has an accelerating effect on NADH oxidation. Cinnamyl alcohol polymerizing activity of POX increased with disease progression and was strongly inhibited by H2O2. Stimulation of these reactions by cofactors has a physiological significance in terms of susceptibility of the host.</abstract><doi>10.17221/120/2009-PSE</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1214-1178 |
| ispartof | Plant, soil and environment, 2010-01, Vol.56 (9) |
| issn | 1214-1178 |
| language | eng |
| recordid | cdi_fao_agris_CZ2010000779 |
| source | DOAJ Directory of Open Access Journals |
| subjects | ACACIA ACACIA EBURNEA BIOCHEMICAL PATHWAYS BIOCHEMICAL REACTIONS CHAMPIGNON ENFERMEDADES FUNGOSAS FUNGAL DISEASES FUNGI HONGOS http://www.fao.org/aos/agrovoc#c_11042 http://www.fao.org/aos/agrovoc#c_13251 http://www.fao.org/aos/agrovoc#c_25446 http://www.fao.org/aos/agrovoc#c_27462 http://www.fao.org/aos/agrovoc#c_3145 http://www.fao.org/aos/agrovoc#c_32 http://www.fao.org/aos/agrovoc#c_3725 http://www.fao.org/aos/agrovoc#c_4328 http://www.fao.org/aos/agrovoc#c_6710 http://www.fao.org/aos/agrovoc#c_909 HYDROGEN PEROXIDE LIGNIFICACION LIGNIFICATION MALADIE FONGIQUE PEROXIDASAS PEROXIDASES PEROXIDO DE HIDROGENO PEROXYDASE PEROXYDE D'HYDROGENE PLANT RESPONSE RAVENELIA ESCULENTA REACCIONES BIOQUIMICAS REACTION BIOCHIMIQUE REPONSE DE LA PLANTE RESPUESTA DE LA PLANTA ROUILLE ROYA RUSTS VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME |
| title | Hydrogen peroxide generation and lignification by peroxidases from Acacia eburnea infected with Ravenelia esculenta |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T15%3A43%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hydrogen%20peroxide%20generation%20and%20lignification%20by%20peroxidases%20from%20Acacia%20eburnea%20infected%20with%20Ravenelia%20esculenta&rft.jtitle=Plant,%20soil%20and%20environment&rft.au=Kuvalekar,%20A.A.,Modern%20Coll.%20of%20Arts,%20Science%20and%20Commerce,%20Shivajinagar%20(India).%20Dept.%20of%20Botany&rft.date=2010-01-01&rft.volume=56&rft.issue=9&rft.issn=1214-1178&rft_id=info:doi/10.17221/120/2009-PSE&rft_dat=%3Cfao%3ECZ2010000779%3C/fao%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |