Segregation based on the traits and molecular marker of near-isogenic line of sorghum bicolor×sorghum sudanenes

Segregation based on the traits and molecular marker of near-isogenic line of sorghum bicolor×sorghum sudanenes

【目的】以株高等表型性状和分子标记为基础,从高粱×苏丹草的F6:7重组自交系群体中分离株高、叶片数2个性状及对这2个性状有效应的分子标记的近等基因系,为精确定位性状的QTL及分析QTL的作用提供有用的遗传材料。【方法】利用高粱314A×棕壳苏丹草的F2:3遗传作图群体的后代材料建立重组自交系群体F6:7,对株高、叶片数2个性状,采用基于性状和基于分子标记的近等基因系分离法,分别找出差异显著的株系和杂合的株系。将2种方法找出的共同株系再进行SSR标记,剔除杂合株,其余2种纯合子基因型即为该性状的近等基因系（NIL）。【结果】在重组自交系RIL-105和RIL-85家系内分别分离出了株高和叶片数的...

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Veröffentlicht in:Chung-kuo nung yeh kʿo hsüeh 2010-02, Vol.43 (3)
Hauptverfasser: Lu Xiaoping, Agronomy College of Inner Mongolia Agricultural University, Huhehaote(China), Yun Jinfeng, Agronomy College of Inner Mongolia Agricultural University, Huhehaote(China), Mi Fugui, Agronomy College of Inner Mongolia Agricultural University, Huhehaote(China)
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Sprache:chi
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http://www.fao.org/aos/agrovoc#c_24039
http://www.fao.org/aos/agrovoc#c_7244
http://www.fao.org/aos/agrovoc#c_7246
INBRED LINES
LIGNEE CONSANGUINE
LINEAS CONSANGUINEAS
SORGHUM
SORGHUM ARUNDINACEUM
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Zusammenfassung:【目的】以株高等表型性状和分子标记为基础,从高粱×苏丹草的F6:7重组自交系群体中分离株高、叶片数2个性状及对这2个性状有效应的分子标记的近等基因系,为精确定位性状的QTL及分析QTL的作用提供有用的遗传材料。【方法】利用高粱314A×棕壳苏丹草的F2:3遗传作图群体的后代材料建立重组自交系群体F6:7,对株高、叶片数2个性状,采用基于性状和基于分子标记的近等基因系分离法,分别找出差异显著的株系和杂合的株系。将2种方法找出的共同株系再进行SSR标记,剔除杂合株,其余2种纯合子基因型即为该性状的近等基因系（NIL）。【结果】在重组自交系RIL-105和RIL-85家系内分别分离出了株高和叶片数的近等基因系。在重组自交系RIL-127家系内分离出了互作位点umc1714―umc2247的近等基因系。【结论】利用分离得到的这些近等基因系可进一步精确定位性状的QTL位点和分离QTL位点及对互作位点（上位性）的遗传效应进行分析。 【Objective】 Based on the traits and the molecular markers of Sorghum (Sorghum bicolor)× Sudan grass (Sorghum sudanenes), the near-isogenic lines (NILs) of plant height, leaf number and related molecular markers were determined from the recombinant inbred line (RIL) F6:7 family. The material could be used for the QTLs analysis. 【Method】 The recombinant inbred line F6:7 family came from F2:3 genetic mapping population of Sorghum314A×Brown Sudan grass,to the traits of plant height and leaf number, the NILs were isolated separately based on the traits and the molecular markers. The plants with one trait d
ISSN:0578-1752