IMPROVEMENTS TO THE TECHNIQUES OF AMPLIFICATION OF NUCLEIC ACID

Improvement relating to techniques for the amplification of nucleic acids, in particular by polymerization chain reaction (PCR). Said process for the detection and/or the identification of a nucleic acid sequence or of a mixture of nucleic acid sequences once the bioligical sample has been placed in an appropriate solution in order to extract the nucleic acid or acids, includes the following steps: (1) the destruction of the 5' ends of the nucleic acid sequences with double strand which are present in the sample, by bringing said biological sample into contact with an appropriate reaction agent which is active at a temperature of between 37 DEG - 42 DEG C; (2) the actual amplification by bringing the resulting sample into contact with (1) appropriate reagents, in particular amplification initiators suitable for the amplification of the target sequence or sequences to be detected, in the presence of a heat-resistant polymerase DNA; (3) detection of the specific amplified target sequences.