Method for the design of oligonucleotides for molecular biology techniques

Designing oligonucleotides used in molecular biology process comprises selecting/creating a data base of reference sequences, i.e. an evaluating data base, selecting a subset of sequences of a desired organism to create a reference data base, i.e. a design data base, selecting oligonucleotides to be...

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Hauptverfasser: ARAVENA, DUARTE ANDRES OCTAVIO, MARTINEZ, AGUILERA SERVET, PARADA, VALDECANTOS PILAR ANGELICA, GONZALEZ, CANALES MAURICIO ALEJANDRO, EHRENFELD , STOLZENBACH KATIA NICOLE, MAASS, SEPULVEDA ALEJANDRO EDUARDO
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creator ARAVENA, DUARTE ANDRES OCTAVIO
MARTINEZ, AGUILERA SERVET
PARADA, VALDECANTOS PILAR ANGELICA
GONZALEZ, CANALES MAURICIO ALEJANDRO
EHRENFELD , STOLZENBACH KATIA NICOLE
MAASS, SEPULVEDA ALEJANDRO EDUARDO
description Designing oligonucleotides used in molecular biology process comprises selecting/creating a data base of reference sequences, i.e. an evaluating data base, selecting a subset of sequences of a desired organism to create a reference data base, i.e. a design data base, selecting oligonucleotides to be examined from the subset of sequences, purifying the selected oligonucleotides as per the criteria of hybridization specificity and thermodynamic stability and obtaining a list of oligonucleotides that satisfies all the above conditions to give oligonucleotides obtained by the method.
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PHYSICS
title Method for the design of oligonucleotides for molecular biology techniques
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