A METHOD OF REMOVING NUCLEIC ACID CONTAMINATION IN AMPLIFICATION REACTIONS

The present invention provides a method of removing nucleic acid contamination in an amplification reaction which comprises use of a thermolabile DNase and a method of preventing or reducing false positive results due to carry-over in a nucleic acid amplification reaction, said method comprising usi...

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Hauptverfasser:	SANDSDALEN, ERLING, STENBERG, EVEN, NILSEN, INGE, WALLER
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a method of removing nucleic acid contamination in an amplification reaction which comprises use of a thermolabile DNase and a method of preventing or reducing false positive results due to carry-over in a nucleic acid amplification reaction, said method comprising using a thermolabile DNase to degrade carried-over non-target double-stranded DNA present in the amplification reaction mixture. A thermolabile DNase from the shrimp Pandalus borealis has been identified which is suitable for use in the methods of the invention. La présente invention concerne une méthode d'élimination de la contamination de l'acide nucléique dans une réaction d'amplification consistant à utiliser une DNase thermolabile et une méthode permettant d'éviter ou de réduire l'obtention de résultats positifs faux, dus à une contamination dans une réaction d'amplification d'un acide nucléique, ladite méthode consistant à utiliser une DNase thermolabile pour dégrader l'ADN double brin, non cible, contaminé, présent dans le mélange de réaction d'amplification. Une DNase thermolabile de la crevette Pandalus borealis a été identifiée, laquelle convient bien à l'utilisation dans les méthodes de la présente invention.