PLANT MICROPROPAGATION AND GERMPLASM STORAGE

The present invention relates to a method for the in vitro preparation of starting materials for micropropagation and/or storage of germplasm, comprising the steps of contacting plant material with a microorganism that induces fasciation and/or one or more fasciation-inducing factors derived from th...

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Hauptverfasser: EL JAZIRI, MONDHER, VAN MONTAGU, MARC, CHARLES, ERNEST, GOETHALS, KOENRAAD, HILAIRE, MARIA
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creator EL JAZIRI, MONDHER
VAN MONTAGU, MARC, CHARLES, ERNEST
GOETHALS, KOENRAAD, HILAIRE, MARIA
description The present invention relates to a method for the in vitro preparation of starting materials for micropropagation and/or storage of germplasm, comprising the steps of contacting plant material with a microorganism that induces fasciation and/or one or more fasciation-inducing factors derived from the microorganism or derived from the infected and/or fasciated tissue; developing leafy galls or shoot outgrowths on the plant material; and isolating the leafy galls or shoot outgrowths as the starting materials. This method may be used for the in vitro micropropagation of plants, which further comprises the steps of eliminating or inactivating the microorganism that induces fasciation and/or the one or more fasciation-related factors derived from the microorganism or derived from the infected and/or fasciated tissue; culturing the leafy gall or shoot outgrowths in or on one or more suitable culture media to allow shoot and root development for obtaining plantlets; and transferring the plantlets thus obtained, optionally after acclimatization, to conventional growing conditions to obtain regenerated plants. The method may also be used for the preservation of plants or germplasm, which is further characterized by the step of storing the leafy galls or shoot outgrowths under growth limiting conditions.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
title PLANT MICROPROPAGATION AND GERMPLASM STORAGE
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