HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS
The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the ta...
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| creator | REGNIER, FRED, E WILLIAMS, KEVIN AFEYAN, NOUBAR, B EVANS, DAVID PINGALI, ARUNA JINDAL, SATISH PALIWAL, SANDEEP |
| description | The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second "dimension". The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
L'invention concerne des procédés nouveaux de criblage d'un échantillon, destinés à sélectionner un ligand d'une cible d'intérêt et à obtenir des renseignements relatifs audit ligand ainsi qu'à ses caractéristiques de liaison. Plus particulièrement, les procédés multidimensionnels revendiqués consistent à combiner une solution de ligands hétérogènes avec la cible d'intérêt afin de cribler les ligands sur la base d'une ou de plusieurs caractéristiques de liaison. Les ligands possédant la première caractéristiques de liaison se lient à la cible d'intérêt, formant ainsi un complexe cible/ligand. Ledit complexe est ensuite éventuellement séparé des composants non liés à l'aide de l'une des diverses techniques de séparation telles que, par exemple, la technique d'exclusion par la taille. Au moins un des composants complexes ou non liés est ensuite soumis à une seconde "dimension", capable de séparer les composants sur la base d'une deuxième caractéristique de liaison. On élue ensuite le ligand ayant les caractéristiques de liaison désirées. |
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L'invention concerne des procédés nouveaux de criblage d'un échantillon, destinés à sélectionner un ligand d'une cible d'intérêt et à obtenir des renseignements relatifs audit ligand ainsi qu'à ses caractéristiques de liaison. Plus particulièrement, les procédés multidimensionnels revendiqués consistent à combiner une solution de ligands hétérogènes avec la cible d'intérêt afin de cribler les ligands sur la base d'une ou de plusieurs caractéristiques de liaison. Les ligands possédant la première caractéristiques de liaison se lient à la cible d'intérêt, formant ainsi un complexe cible/ligand. Ledit complexe est ensuite éventuellement séparé des composants non liés à l'aide de l'une des diverses techniques de séparation telles que, par exemple, la technique d'exclusion par la taille. Au moins un des composants complexes ou non liés est ensuite soumis à une seconde "dimension", capable de séparer les composants sur la base d'une deuxième caractéristique de liaison. On élue ensuite le ligand ayant les caractéristiques de liaison désirées.</description><edition>6</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY ; CHEMISTRY ; COMBINATORIAL CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; THEIR RELEVANT APPARATUS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>1997</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19970116&DB=EPODOC&CC=WO&NR=9701755A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19970116&DB=EPODOC&CC=WO&NR=9701755A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>REGNIER, FRED, E</creatorcontrib><creatorcontrib>WILLIAMS, KEVIN</creatorcontrib><creatorcontrib>AFEYAN, NOUBAR, B</creatorcontrib><creatorcontrib>EVANS, DAVID</creatorcontrib><creatorcontrib>PINGALI, ARUNA</creatorcontrib><creatorcontrib>JINDAL, SATISH</creatorcontrib><creatorcontrib>PALIWAL, SANDEEP</creatorcontrib><title>HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS</title><description>The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second "dimension". The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
L'invention concerne des procédés nouveaux de criblage d'un échantillon, destinés à sélectionner un ligand d'une cible d'intérêt et à obtenir des renseignements relatifs audit ligand ainsi qu'à ses caractéristiques de liaison. Plus particulièrement, les procédés multidimensionnels revendiqués consistent à combiner une solution de ligands hétérogènes avec la cible d'intérêt afin de cribler les ligands sur la base d'une ou de plusieurs caractéristiques de liaison. Les ligands possédant la première caractéristiques de liaison se lient à la cible d'intérêt, formant ainsi un complexe cible/ligand. Ledit complexe est ensuite éventuellement séparé des composants non liés à l'aide de l'une des diverses techniques de séparation telles que, par exemple, la technique d'exclusion par la taille. Au moins un des composants complexes ou non liés est ensuite soumis à une seconde "dimension", capable de séparer les composants sur la base d'une deuxième caractéristique de liaison. On élue ensuite le ligand ayant les caractéristiques de liaison désirées.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMBINATORIAL CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>THEIR RELEVANT APPARATUS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1997</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZIjy8HT3UAgOcHV10VFwDA3x93UMATGd_f1CPP1C_UODFdx8_MN1FHxDfUI8dV08fV39gj39_Rx9FHz9fVydQ30cgxSCXYGsEKCogqOfCxA7-kQGewbzMLCmJeYUp_JCaW4GBTfXEGcP3dSC_PjU4oLE5NS81JL4cH9LcwNDc1NTRyNjIpQAAPdAMaU</recordid><startdate>19970116</startdate><enddate>19970116</enddate><creator>REGNIER, FRED, E</creator><creator>WILLIAMS, KEVIN</creator><creator>AFEYAN, NOUBAR, B</creator><creator>EVANS, DAVID</creator><creator>PINGALI, ARUNA</creator><creator>JINDAL, SATISH</creator><creator>PALIWAL, SANDEEP</creator><scope>EVB</scope></search><sort><creationdate>19970116</creationdate><title>HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS</title><author>REGNIER, FRED, E ; WILLIAMS, KEVIN ; AFEYAN, NOUBAR, B ; EVANS, DAVID ; PINGALI, ARUNA ; JINDAL, SATISH ; PALIWAL, SANDEEP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO9701755A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>1997</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMBINATORIAL CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>THEIR RELEVANT APPARATUS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>REGNIER, FRED, E</creatorcontrib><creatorcontrib>WILLIAMS, KEVIN</creatorcontrib><creatorcontrib>AFEYAN, NOUBAR, B</creatorcontrib><creatorcontrib>EVANS, DAVID</creatorcontrib><creatorcontrib>PINGALI, ARUNA</creatorcontrib><creatorcontrib>JINDAL, SATISH</creatorcontrib><creatorcontrib>PALIWAL, SANDEEP</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>REGNIER, FRED, E</au><au>WILLIAMS, KEVIN</au><au>AFEYAN, NOUBAR, B</au><au>EVANS, DAVID</au><au>PINGALI, ARUNA</au><au>JINDAL, SATISH</au><au>PALIWAL, SANDEEP</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS</title><date>1997-01-16</date><risdate>1997</risdate><abstract>The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second "dimension". The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
L'invention concerne des procédés nouveaux de criblage d'un échantillon, destinés à sélectionner un ligand d'une cible d'intérêt et à obtenir des renseignements relatifs audit ligand ainsi qu'à ses caractéristiques de liaison. Plus particulièrement, les procédés multidimensionnels revendiqués consistent à combiner une solution de ligands hétérogènes avec la cible d'intérêt afin de cribler les ligands sur la base d'une ou de plusieurs caractéristiques de liaison. Les ligands possédant la première caractéristiques de liaison se lient à la cible d'intérêt, formant ainsi un complexe cible/ligand. Ledit complexe est ensuite éventuellement séparé des composants non liés à l'aide de l'une des diverses techniques de séparation telles que, par exemple, la technique d'exclusion par la taille. Au moins un des composants complexes ou non liés est ensuite soumis à une seconde "dimension", capable de séparer les composants sur la base d'une deuxième caractéristique de liaison. On élue ensuite le ligand ayant les caractéristiques de liaison désirées.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE |
| title | HIGH SPEED, AUTOMATED, CONTINUOUS FLOW, MULTI-DIMENSIONAL MOLECULAR SELECTION AND ANALYSIS |
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