A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR
The present invention describes a highly sensitive and specific non-radioactive quantitative assay method for quantifying transforming growth factor-beta (TGF- beta ) in a liquid sample. Also disclosed are TGF- beta responsive expression vectors that express the indicator molecule, luciferase, in a...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | CURRIDEN, SCOTT, A LOCKUTOFF, DAVID, J |
| description | The present invention describes a highly sensitive and specific non-radioactive quantitative assay method for quantifying transforming growth factor-beta (TGF- beta ) in a liquid sample. Also disclosed are TGF- beta responsive expression vectors that express the indicator molecule, luciferase, in a dose-dependent response to TGF- beta activation. Eucaryotic cells transformed with the disclosed expression vectors are also described. Diagnostic systems in the form of kits for quantifying the amount of TGF- beta in a liquid sample using the disclosed methods and expression vectors are described.
La présente invention concerne une méthode de dosage quantitative, non radioactive, spécifique et très sensible servant à quantifier le facteur de croissance transformant bêta (TGF- beta ) dans un échantillon liquide. L'invention concerne également des vecteurs d'expression sensibles au TGF- beta qui expriment la molécule indicatrice, la luciférase, dans une réponse, reliée à la dose, à l'activation du TGF- beta . L'invention concerne en outre des cellules eucaryotes transformées par les vecteurs d'expression décrits, et des systèmes de diagnostic se présentant sous la forme de kits permettant de déterminer la quantité de TGF- beta dans un échantillon liquide à l'aide des procédés et des vecteurs d'expression décrits. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO9519987A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO9519987A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO9519987A13</originalsourceid><addsrcrecordid>eNqNjDEOgkAQRWksjHqHuQAFMUYpR5hlt2BGd0eJFSFmtTFKgvePYDyA1U_-e__PkzsCUwOBODh1Z4Ka1EoJRjwcT8jqzMVxBVh8qXrkMLJ66iovjVowIxOf7kkRkEsopD7I9CYcQC15GgfLZHbrHkNc_XKRgCEtbBr7VxuHvrvGZ3y3jeSbLM93W8zWfygfun81pg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR</title><source>esp@cenet</source><creator>CURRIDEN, SCOTT, A ; LOCKUTOFF, DAVID, J</creator><creatorcontrib>CURRIDEN, SCOTT, A ; LOCKUTOFF, DAVID, J</creatorcontrib><description>The present invention describes a highly sensitive and specific non-radioactive quantitative assay method for quantifying transforming growth factor-beta (TGF- beta ) in a liquid sample. Also disclosed are TGF- beta responsive expression vectors that express the indicator molecule, luciferase, in a dose-dependent response to TGF- beta activation. Eucaryotic cells transformed with the disclosed expression vectors are also described. Diagnostic systems in the form of kits for quantifying the amount of TGF- beta in a liquid sample using the disclosed methods and expression vectors are described.
La présente invention concerne une méthode de dosage quantitative, non radioactive, spécifique et très sensible servant à quantifier le facteur de croissance transformant bêta (TGF- beta ) dans un échantillon liquide. L'invention concerne également des vecteurs d'expression sensibles au TGF- beta qui expriment la molécule indicatrice, la luciférase, dans une réponse, reliée à la dose, à l'activation du TGF- beta . L'invention concerne en outre des cellules eucaryotes transformées par les vecteurs d'expression décrits, et des systèmes de diagnostic se présentant sous la forme de kits permettant de déterminer la quantité de TGF- beta dans un échantillon liquide à l'aide des procédés et des vecteurs d'expression décrits.</description><edition>6</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1995</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19950727&DB=EPODOC&CC=WO&NR=9519987A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19950727&DB=EPODOC&CC=WO&NR=9519987A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CURRIDEN, SCOTT, A</creatorcontrib><creatorcontrib>LOCKUTOFF, DAVID, J</creatorcontrib><title>A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR</title><description>The present invention describes a highly sensitive and specific non-radioactive quantitative assay method for quantifying transforming growth factor-beta (TGF- beta ) in a liquid sample. Also disclosed are TGF- beta responsive expression vectors that express the indicator molecule, luciferase, in a dose-dependent response to TGF- beta activation. Eucaryotic cells transformed with the disclosed expression vectors are also described. Diagnostic systems in the form of kits for quantifying the amount of TGF- beta in a liquid sample using the disclosed methods and expression vectors are described.
La présente invention concerne une méthode de dosage quantitative, non radioactive, spécifique et très sensible servant à quantifier le facteur de croissance transformant bêta (TGF- beta ) dans un échantillon liquide. L'invention concerne également des vecteurs d'expression sensibles au TGF- beta qui expriment la molécule indicatrice, la luciférase, dans une réponse, reliée à la dose, à l'activation du TGF- beta . L'invention concerne en outre des cellules eucaryotes transformées par les vecteurs d'expression décrits, et des systèmes de diagnostic se présentant sous la forme de kits permettant de déterminer la quantité de TGF- beta dans un échantillon liquide à l'aide des procédés et des vecteurs d'expression décrits.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1995</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEOgkAQRWksjHqHuQAFMUYpR5hlt2BGd0eJFSFmtTFKgvePYDyA1U_-e__PkzsCUwOBODh1Z4Ka1EoJRjwcT8jqzMVxBVh8qXrkMLJ66iovjVowIxOf7kkRkEsopD7I9CYcQC15GgfLZHbrHkNc_XKRgCEtbBr7VxuHvrvGZ3y3jeSbLM93W8zWfygfun81pg</recordid><startdate>19950727</startdate><enddate>19950727</enddate><creator>CURRIDEN, SCOTT, A</creator><creator>LOCKUTOFF, DAVID, J</creator><scope>EVB</scope></search><sort><creationdate>19950727</creationdate><title>A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR</title><author>CURRIDEN, SCOTT, A ; LOCKUTOFF, DAVID, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO9519987A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>1995</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CURRIDEN, SCOTT, A</creatorcontrib><creatorcontrib>LOCKUTOFF, DAVID, J</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CURRIDEN, SCOTT, A</au><au>LOCKUTOFF, DAVID, J</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR</title><date>1995-07-27</date><risdate>1995</risdate><abstract>The present invention describes a highly sensitive and specific non-radioactive quantitative assay method for quantifying transforming growth factor-beta (TGF- beta ) in a liquid sample. Also disclosed are TGF- beta responsive expression vectors that express the indicator molecule, luciferase, in a dose-dependent response to TGF- beta activation. Eucaryotic cells transformed with the disclosed expression vectors are also described. Diagnostic systems in the form of kits for quantifying the amount of TGF- beta in a liquid sample using the disclosed methods and expression vectors are described.
La présente invention concerne une méthode de dosage quantitative, non radioactive, spécifique et très sensible servant à quantifier le facteur de croissance transformant bêta (TGF- beta ) dans un échantillon liquide. L'invention concerne également des vecteurs d'expression sensibles au TGF- beta qui expriment la molécule indicatrice, la luciférase, dans une réponse, reliée à la dose, à l'activation du TGF- beta . L'invention concerne en outre des cellules eucaryotes transformées par les vecteurs d'expression décrits, et des systèmes de diagnostic se présentant sous la forme de kits permettant de déterminer la quantité de TGF- beta dans un échantillon liquide à l'aide des procédés et des vecteurs d'expression décrits.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO9519987A1 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | A NEW SENSITIVE METHOD FOR QUANTIFYING ACTIVE TRANSFORMING GROWTH FACTOR-BETA AND COMPOSITIONS THEREFOR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T11%3A30%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=CURRIDEN,%20SCOTT,%20A&rft.date=1995-07-27&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO9519987A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |