REVERSE TRANSCRIPTASE WITH IMPROVED PROPERTIES

The present invention relates to reverse transcriptase enzymes comprising a modified Moloney Murine Leukaemia Virus reverse transcriptase amino acid sequence, wherein said amino acid sequence comprises a mutation compared to the wild-type Moloney Murine Leukaemia Virus reverse transcriptase, and sai...

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Hauptverfasser: RIMSELIENE, Renata, ZVIRBLIENE, Marija, VIRKUTIS, Jonas
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creator RIMSELIENE, Renata
ZVIRBLIENE, Marija
VIRKUTIS, Jonas
description The present invention relates to reverse transcriptase enzymes comprising a modified Moloney Murine Leukaemia Virus reverse transcriptase amino acid sequence, wherein said amino acid sequence comprises a mutation compared to the wild-type Moloney Murine Leukaemia Virus reverse transcriptase, and said enzyme possesses increased inhibitor resistance compared to the wild-type Moloney Murine Leukaemia Virus reverse transcriptase. The present invention also relates to compositions, reaction mixtures, and kits comprising the said reverse transcriptase enzymes are used to synthesize cDNA from RNA. La présente invention concerne des enzymes de transcriptase inverse comprenant une séquence d'acides aminés de la transcriptase inverse du virus de la leucémie murine de Moloney modifiée, ladite séquence d'acides aminés contenant une mutation par comparaison avec la transcriptase inverse du virus de la leucémie murine de Moloney de type sauvage, et ladite enzyme possède une résistance accrue aux inhibiteurs par comparaison avec la transcriptase inverse du virus de la leucémie murine de Moloney de type sauvage. La présente invention concerne également des compositions, des mélanges réactionnels et des kits contenant lesdites enzymes de transcriptase inverse étant utilisés pour synthétiser l'ADNc à partir de l'ARN.
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The present invention also relates to compositions, reaction mixtures, and kits comprising the said reverse transcriptase enzymes are used to synthesize cDNA from RNA. La présente invention concerne des enzymes de transcriptase inverse comprenant une séquence d'acides aminés de la transcriptase inverse du virus de la leucémie murine de Moloney modifiée, ladite séquence d'acides aminés contenant une mutation par comparaison avec la transcriptase inverse du virus de la leucémie murine de Moloney de type sauvage, et ladite enzyme possède une résistance accrue aux inhibiteurs par comparaison avec la transcriptase inverse du virus de la leucémie murine de Moloney de type sauvage. La présente invention concerne également des compositions, des mélanges réactionnels et des kits contenant lesdites enzymes de transcriptase inverse étant utilisés pour synthétiser l'ADNc à partir de l'ARN.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title REVERSE TRANSCRIPTASE WITH IMPROVED PROPERTIES
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