CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM
The present invention relates to a chimeric protein and a biosensor system based on said chimeric protein that enable the binding of an extracellular target antigen and the generation of a signal upon recognition. The chimeric protein of the present invention comprises (i) an antigen binding domain,...
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| creator | CEBALLOS MUNUERA, Álvaro FERNÁNDEZ HERRERO, Luis Ángel PRIETO DURÁN, Alejandro |
| description | The present invention relates to a chimeric protein and a biosensor system based on said chimeric protein that enable the binding of an extracellular target antigen and the generation of a signal upon recognition. The chimeric protein of the present invention comprises (i) an antigen binding domain, (ii) at least one β-barrel domain from an autotransporter, and (iii) a reporter protein domain comprising a partner of a split protein luminescence system, wherein domain (ii) is bound to domain (i) by one end and to domain (iii) by the other end. The present invention also discloses a host cell comprising at least two chimeric proteins of the invention complementary each other as an in vitro antigen detection system.
La présente invention concerne une protéine chimérique et un système de biocapteur basé sur ladite protéine chimérique qui permettent la liaison d'un antigène cible extracellulaire et la génération d'un signal lors de la reconnaissance. La protéine chimérique selon la présente invention comprend (i) un domaine de liaison à l'antigène, (ii) au moins un domaine β-barrel provenant d'un autotransporteur, et (iii) un domaine de protéine rapporteur comprenant un partenaire d'un système de luminescence de protéine divisée, le domaine (ii) étant lié au domaine (i) par une extrémité et au domaine (iii) par l'autre extrémité. La présente invention concerne également une cellule hôte comprenant au moins deux protéines chimériques selon l'invention complémentaires l'une de l'autre en tant que système de détection d'antigène in vitro. |
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La présente invention concerne une protéine chimérique et un système de biocapteur basé sur ladite protéine chimérique qui permettent la liaison d'un antigène cible extracellulaire et la génération d'un signal lors de la reconnaissance. La protéine chimérique selon la présente invention comprend (i) un domaine de liaison à l'antigène, (ii) au moins un domaine β-barrel provenant d'un autotransporteur, et (iii) un domaine de protéine rapporteur comprenant un partenaire d'un système de luminescence de protéine divisée, le domaine (ii) étant lié au domaine (i) par une extrémité et au domaine (iii) par l'autre extrémité. La présente invention concerne également une cellule hôte comprenant au moins deux protéines chimériques selon l'invention complémentaires l'une de l'autre en tant que système de détection d'antigène in vitro.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240829&DB=EPODOC&CC=WO&NR=2024175675A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240829&DB=EPODOC&CC=WO&NR=2024175675A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CEBALLOS MUNUERA, Álvaro</creatorcontrib><creatorcontrib>FERNÁNDEZ HERRERO, Luis Ángel</creatorcontrib><creatorcontrib>PRIETO DURÁN, Alejandro</creatorcontrib><title>CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM</title><description>The present invention relates to a chimeric protein and a biosensor system based on said chimeric protein that enable the binding of an extracellular target antigen and the generation of a signal upon recognition. The chimeric protein of the present invention comprises (i) an antigen binding domain, (ii) at least one β-barrel domain from an autotransporter, and (iii) a reporter protein domain comprising a partner of a split protein luminescence system, wherein domain (ii) is bound to domain (i) by one end and to domain (iii) by the other end. The present invention also discloses a host cell comprising at least two chimeric proteins of the invention complementary each other as an in vitro antigen detection system.
La présente invention concerne une protéine chimérique et un système de biocapteur basé sur ladite protéine chimérique qui permettent la liaison d'un antigène cible extracellulaire et la génération d'un signal lors de la reconnaissance. La protéine chimérique selon la présente invention comprend (i) un domaine de liaison à l'antigène, (ii) au moins un domaine β-barrel provenant d'un autotransporteur, et (iii) un domaine de protéine rapporteur comprenant un partenaire d'un système de luminescence de protéine divisée, le domaine (ii) étant lié au domaine (i) par une extrémité et au domaine (iii) par l'autre extrémité. La présente invention concerne également une cellule hôte comprenant au moins deux protéines chimériques selon l'invention complémentaires l'une de l'autre en tant que système de détection d'antigène in vitro.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLBz9vD0dQ3ydFYICPIPcfX0C1Zw9HNRCPFw9QxSCA12VXAECQBRiKe7q5-Ci2uIq3OIp5-7QnBkcIirLw8Da1piTnEqL5TmZlB2cw1x9tBNLciPTy0uSExOzUstiQ_3NzIwMjE0NzUzN3U0NCZOFQDjOirc</recordid><startdate>20240829</startdate><enddate>20240829</enddate><creator>CEBALLOS MUNUERA, Álvaro</creator><creator>FERNÁNDEZ HERRERO, Luis Ángel</creator><creator>PRIETO DURÁN, Alejandro</creator><scope>EVB</scope></search><sort><creationdate>20240829</creationdate><title>CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM</title><author>CEBALLOS MUNUERA, Álvaro ; FERNÁNDEZ HERRERO, Luis Ángel ; PRIETO DURÁN, Alejandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2024175675A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CEBALLOS MUNUERA, Álvaro</creatorcontrib><creatorcontrib>FERNÁNDEZ HERRERO, Luis Ángel</creatorcontrib><creatorcontrib>PRIETO DURÁN, Alejandro</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CEBALLOS MUNUERA, Álvaro</au><au>FERNÁNDEZ HERRERO, Luis Ángel</au><au>PRIETO DURÁN, Alejandro</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM</title><date>2024-08-29</date><risdate>2024</risdate><abstract>The present invention relates to a chimeric protein and a biosensor system based on said chimeric protein that enable the binding of an extracellular target antigen and the generation of a signal upon recognition. The chimeric protein of the present invention comprises (i) an antigen binding domain, (ii) at least one β-barrel domain from an autotransporter, and (iii) a reporter protein domain comprising a partner of a split protein luminescence system, wherein domain (ii) is bound to domain (i) by one end and to domain (iii) by the other end. The present invention also discloses a host cell comprising at least two chimeric proteins of the invention complementary each other as an in vitro antigen detection system.
La présente invention concerne une protéine chimérique et un système de biocapteur basé sur ladite protéine chimérique qui permettent la liaison d'un antigène cible extracellulaire et la génération d'un signal lors de la reconnaissance. La protéine chimérique selon la présente invention comprend (i) un domaine de liaison à l'antigène, (ii) au moins un domaine β-barrel provenant d'un autotransporteur, et (iii) un domaine de protéine rapporteur comprenant un partenaire d'un système de luminescence de protéine divisée, le domaine (ii) étant lié au domaine (i) par une extrémité et au domaine (iii) par l'autre extrémité. La présente invention concerne également une cellule hôte comprenant au moins deux protéines chimériques selon l'invention complémentaires l'une de l'autre en tant que système de détection d'antigène in vitro.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM |
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