ENZYME VARIANTS AND USES THEREOF
The present disclosure relates generally to polypeptides capable of hydrolysing an amide bond in a polyamide, and more specifically to polypeptides comprising an amino acid sequence of amino acid residues 2-398 of SEQ ID NO: 2 or an amino acid sequence that has at least 70% sequence identity thereto...
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| creator | VONGSOUTHI, Vanessa TON, Jennifer Nhu Tuyet Nu MATTHEWS, Dana Suzanne JACKSON, Colin John SPENCE, Matthew MATTHEWS, Christopher Jonathan |
| description | The present disclosure relates generally to polypeptides capable of hydrolysing an amide bond in a polyamide, and more specifically to polypeptides comprising an amino acid sequence of amino acid residues 2-398 of SEQ ID NO: 2 or an amino acid sequence that has at least 70% sequence identity thereto; or polypeptides comprising an amino acid sequence of amino acid residues 2-394 of SEQ ID NO:88 or an amino acid sequence that has at least 75% sequence identity thereto. The present disclosure also extends to methods of using said polypeptides. In another embodiment, the polypeptides disclosed are capable of hydrolysing the nylon 6,6 oligomer to produce adipic acid and hexamethylenediamine.
La présente divulgation concerne d'une manière générale des polypeptides pouvant hydrolyser une liaison amide dans un polyamide, et plus particulièrement des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 398 de SEQ ID NO : 2 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 70 % ; ou des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 394 de SEQ ID NO : 88 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 75 %. La présente invention concerne également des procédés d'utilisation desdits polypeptides. Selon un autre mode de réalisation, les polypeptides divulgués peuvent hydrolyser l'oligomère de nylon 6,6 pour produire de l'acide adipique et de l'hexaméthylènediamine. |
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La présente divulgation concerne d'une manière générale des polypeptides pouvant hydrolyser une liaison amide dans un polyamide, et plus particulièrement des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 398 de SEQ ID NO : 2 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 70 % ; ou des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 394 de SEQ ID NO : 88 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 75 %. La présente invention concerne également des procédés d'utilisation desdits polypeptides. Selon un autre mode de réalisation, les polypeptides divulgués peuvent hydrolyser l'oligomère de nylon 6,6 pour produire de l'acide adipique et de l'hexaméthylènediamine.</description><language>eng ; fre</language><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS BASED THEREON ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; GENERAL PROCESSES OF COMPOUNDING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC MACROMOLECULAR COMPOUNDS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR PREPARATION OR CHEMICAL WORKING-UP ; VINEGAR ; WINE ; WORKING-UP</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240802&DB=EPODOC&CC=WO&NR=2024156025A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240802&DB=EPODOC&CC=WO&NR=2024156025A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VONGSOUTHI, Vanessa</creatorcontrib><creatorcontrib>TON, Jennifer Nhu Tuyet Nu</creatorcontrib><creatorcontrib>MATTHEWS, Dana Suzanne</creatorcontrib><creatorcontrib>JACKSON, Colin John</creatorcontrib><creatorcontrib>SPENCE, Matthew</creatorcontrib><creatorcontrib>MATTHEWS, Christopher Jonathan</creatorcontrib><title>ENZYME VARIANTS AND USES THEREOF</title><description>The present disclosure relates generally to polypeptides capable of hydrolysing an amide bond in a polyamide, and more specifically to polypeptides comprising an amino acid sequence of amino acid residues 2-398 of SEQ ID NO: 2 or an amino acid sequence that has at least 70% sequence identity thereto; or polypeptides comprising an amino acid sequence of amino acid residues 2-394 of SEQ ID NO:88 or an amino acid sequence that has at least 75% sequence identity thereto. The present disclosure also extends to methods of using said polypeptides. In another embodiment, the polypeptides disclosed are capable of hydrolysing the nylon 6,6 oligomer to produce adipic acid and hexamethylenediamine.
La présente divulgation concerne d'une manière générale des polypeptides pouvant hydrolyser une liaison amide dans un polyamide, et plus particulièrement des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 398 de SEQ ID NO : 2 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 70 % ; ou des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 394 de SEQ ID NO : 88 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 75 %. La présente invention concerne également des procédés d'utilisation desdits polypeptides. Selon un autre mode de réalisation, les polypeptides divulgués peuvent hydrolyser l'oligomère de nylon 6,6 pour produire de l'acide adipique et de l'hexaméthylènediamine.</description><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS BASED THEREON</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>GENERAL PROCESSES OF COMPOUNDING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC MACROMOLECULAR COMPOUNDS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WORKING-UP</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFBw9YuK9HVVCHMM8nT0CwlWcPRzUQgNdg1WCPFwDXL1d-NhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGRiaGpmYGRqaOhsbEqQIAf50izQ</recordid><startdate>20240802</startdate><enddate>20240802</enddate><creator>VONGSOUTHI, Vanessa</creator><creator>TON, Jennifer Nhu Tuyet Nu</creator><creator>MATTHEWS, Dana Suzanne</creator><creator>JACKSON, Colin John</creator><creator>SPENCE, Matthew</creator><creator>MATTHEWS, Christopher Jonathan</creator><scope>EVB</scope></search><sort><creationdate>20240802</creationdate><title>ENZYME VARIANTS AND USES THEREOF</title><author>VONGSOUTHI, Vanessa ; TON, Jennifer Nhu Tuyet Nu ; MATTHEWS, Dana Suzanne ; JACKSON, Colin John ; SPENCE, Matthew ; MATTHEWS, Christopher Jonathan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2024156025A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2024</creationdate><topic>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS BASED THEREON</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>GENERAL PROCESSES OF COMPOUNDING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC MACROMOLECULAR COMPOUNDS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR CHEMICAL WORKING-UP</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WORKING-UP</topic><toplevel>online_resources</toplevel><creatorcontrib>VONGSOUTHI, Vanessa</creatorcontrib><creatorcontrib>TON, Jennifer Nhu Tuyet Nu</creatorcontrib><creatorcontrib>MATTHEWS, Dana Suzanne</creatorcontrib><creatorcontrib>JACKSON, Colin John</creatorcontrib><creatorcontrib>SPENCE, Matthew</creatorcontrib><creatorcontrib>MATTHEWS, Christopher Jonathan</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VONGSOUTHI, Vanessa</au><au>TON, Jennifer Nhu Tuyet Nu</au><au>MATTHEWS, Dana Suzanne</au><au>JACKSON, Colin John</au><au>SPENCE, Matthew</au><au>MATTHEWS, Christopher Jonathan</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ENZYME VARIANTS AND USES THEREOF</title><date>2024-08-02</date><risdate>2024</risdate><abstract>The present disclosure relates generally to polypeptides capable of hydrolysing an amide bond in a polyamide, and more specifically to polypeptides comprising an amino acid sequence of amino acid residues 2-398 of SEQ ID NO: 2 or an amino acid sequence that has at least 70% sequence identity thereto; or polypeptides comprising an amino acid sequence of amino acid residues 2-394 of SEQ ID NO:88 or an amino acid sequence that has at least 75% sequence identity thereto. The present disclosure also extends to methods of using said polypeptides. In another embodiment, the polypeptides disclosed are capable of hydrolysing the nylon 6,6 oligomer to produce adipic acid and hexamethylenediamine.
La présente divulgation concerne d'une manière générale des polypeptides pouvant hydrolyser une liaison amide dans un polyamide, et plus particulièrement des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 398 de SEQ ID NO : 2 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 70 % ; ou des polypeptides comprenant une séquence d'acides aminés des résidus d'acides aminés 2 à 394 de SEQ ID NO : 88 ou une séquence d'acides aminés présentant une identité de séquence d'au moins 75 %. La présente invention concerne également des procédés d'utilisation desdits polypeptides. Selon un autre mode de réalisation, les polypeptides divulgués peuvent hydrolyser l'oligomère de nylon 6,6 pour produire de l'acide adipique et de l'hexaméthylènediamine.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY GENERAL PROCESSES OF COMPOUNDING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE WORKING-UP |
| title | ENZYME VARIANTS AND USES THEREOF |
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