INKJET-ASSISTED ENZYMATIC NUCLEIC ACID SYNTHESIS

Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis r...

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Hauptverfasser:	RODRIGUEZ-PINZON, Daniel, ADELIZZI, Beatrice, MOGHADDAM, Nicolas, VERARDO, Damiano, LOMAN, Tessa, HORGAN, Adrian
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMICAL PAINT OR INK REMOVERS CHEMISTRY COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS COLOUR PRINTING CORRECTING FLUIDS DYES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FILLING PASTES INKS LINING MACHINES METALLURGY MICROBIOLOGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS PAINTS PASTES OR SOLIDS FOR COLOURING OR PRINTING PERFORMING OPERATIONS POLISHES PRINTING PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES SPIRITS STAMPS TRANSPORTING TYPEWRITERS USE OF MATERIALS THEREFOR VINEGAR WINE WOODSTAINS
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Beschreibung
Zusammenfassung:	Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides. L'invention concerne des procédés, des dispositifs et des compositions pour la synthèse assistée par jet d'encre d'une pluralité de polynucléotides au niveau de sites de réaction sur un substrat à l'aide de polymérases sans matrice, telles que des désoxynucléotidyl transférases terminales (TdT). Les compositions comprennent des formulations stables imprimables de réactifs de synthèse pour l'administration par jet d'encre comprenant, mais sans s'y limiter, des polymérases sans matrice, des cations divalents et des nucléotides.