RECOMBINANT VECTOR FOR EXPRESSING HETEROLOGOUS PROTEIN, AND METHOD FOR PRODUCING HAND-FOOT-AND-MOUTH VIRUS-LIKE PARTICLE STRUCTURAL PROTEIN BY USING SAME

The present invention relates to a recombinant vector for expressing a heterologous protein, and a method for producing a hand-foot-and-mouth virus-like particle structural protein by using same. The recombinant vector for expressing a heterologous protein, according to the present invention, has be...

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Hauptverfasser:	OH, Mi Kyoung, OH, Jong Min, HAN, Beom Ku, KIM, Hyun Il, LEE, Ji Hoon, WOO, Soo Dong, KIM, Hyun Soo, PARK, Sang Mi, CHOI, Jae Bang, YOON, Bit Na Rae
Format:	Patent
Sprache:	eng ; fre ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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creator	OH, Mi Kyoung OH, Jong Min HAN, Beom Ku KIM, Hyun Il LEE, Ji Hoon WOO, Soo Dong KIM, Hyun Soo PARK, Sang Mi CHOI, Jae Bang YOON, Bit Na Rae
description	The present invention relates to a recombinant vector for expressing a heterologous protein, and a method for producing a hand-foot-and-mouth virus-like particle structural protein by using same. The recombinant vector for expressing a heterologous protein, according to the present invention, has been identified to enable production of hand-foot-and-mouth disease virus-like particles in a high yield by mass-producing of structural proteins and controlling the expression level of 3CD for P1 precursor cleavage, and enables low-cost production of an antigenic protein that is structurally accurate. Therefore, the recombinant vector for expressing a heterologous protein and hand-foot-and-mouth virus-like particles produced using same, according to the present invention, can be variously used in the field of prevention and treatment of hand-foot-and-mouth disease. La présente invention concerne un vecteur recombiné pour l'expression d'une protéine hétérologue, ainsi qu'un procédé de production d'une protéine structurelle de particule du type viral de la fièvre aphteuse à l'aide de ce vecteur. Le vecteur recombiné pour l'expression d'une protéine hétérologue, selon la présente invention, a été identifié pour permettre la production de particules du type viral de la fièvre aphteuse avec un rendement élevé en produisant en masse des protéines structurelles et en maîtrisant le niveau d'expression de la 3CD pour le clivage du précurseur P1, et permet la production à faible coût d'une protéine antigénique structurellement exacte. Par conséquent, le vecteur recombiné pour l'expression d'une protéine hétérologue et les particules du type viral de la fièvre aphteuse produites à l'aide de ce vecteur, selon la présente invention, peuvent être utilisés de diverses manières dans le domaine de la prévention et du traitement de la fièvre aphteuse. 본 발명은 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용한 수족구 바이러스 유사입자 구조 단백질의 생산 방법에 관한 것이다. 본 발명에 따른 외래 단백질 발현을 위한 재조합 벡터는 구조단백질의 대량 생산과 P1 전구체의 절단을 위한 3CD 발현량을 조절함으로써 높은 수율로 수족구병 바이러스 유사입자 생산이 가능함을 확인하였으며, 정확한 구조의 항원 단백질을 저비용으로 생산할 수 있다는 장점이 있다. 따라서 본 발명에 다른 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용하여 생산된 수족구 바이러스 유사입자는 수족구병의 예방 및 치료 분야에서 다양하게 활용될 수 있다.
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The recombinant vector for expressing a heterologous protein, according to the present invention, has been identified to enable production of hand-foot-and-mouth disease virus-like particles in a high yield by mass-producing of structural proteins and controlling the expression level of 3CD for P1 precursor cleavage, and enables low-cost production of an antigenic protein that is structurally accurate. Therefore, the recombinant vector for expressing a heterologous protein and hand-foot-and-mouth virus-like particles produced using same, according to the present invention, can be variously used in the field of prevention and treatment of hand-foot-and-mouth disease. La présente invention concerne un vecteur recombiné pour l'expression d'une protéine hétérologue, ainsi qu'un procédé de production d'une protéine structurelle de particule du type viral de la fièvre aphteuse à l'aide de ce vecteur. Le vecteur recombiné pour l'expression d'une protéine hétérologue, selon la présente invention, a été identifié pour permettre la production de particules du type viral de la fièvre aphteuse avec un rendement élevé en produisant en masse des protéines structurelles et en maîtrisant le niveau d'expression de la 3CD pour le clivage du précurseur P1, et permet la production à faible coût d'une protéine antigénique structurellement exacte. Par conséquent, le vecteur recombiné pour l'expression d'une protéine hétérologue et les particules du type viral de la fièvre aphteuse produites à l'aide de ce vecteur, selon la présente invention, peuvent être utilisés de diverses manières dans le domaine de la prévention et du traitement de la fièvre aphteuse. 본 발명은 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용한 수족구 바이러스 유사입자 구조 단백질의 생산 방법에 관한 것이다. 본 발명에 따른 외래 단백질 발현을 위한 재조합 벡터는 구조단백질의 대량 생산과 P1 전구체의 절단을 위한 3CD 발현량을 조절함으로써 높은 수율로 수족구병 바이러스 유사입자 생산이 가능함을 확인하였으며, 정확한 구조의 항원 단백질을 저비용으로 생산할 수 있다는 장점이 있다. 따라서 본 발명에 다른 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용하여 생산된 수족구 바이러스 유사입자는 수족구병의 예방 및 치료 분야에서 다양하게 활용될 수 있다.</description><language>eng ; fre ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240606&DB=EPODOC&CC=WO&NR=2024117783A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240606&DB=EPODOC&CC=WO&NR=2024117783A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OH, Mi Kyoung</creatorcontrib><creatorcontrib>OH, Jong Min</creatorcontrib><creatorcontrib>HAN, Beom Ku</creatorcontrib><creatorcontrib>KIM, Hyun Il</creatorcontrib><creatorcontrib>LEE, Ji Hoon</creatorcontrib><creatorcontrib>WOO, Soo Dong</creatorcontrib><creatorcontrib>KIM, Hyun Soo</creatorcontrib><creatorcontrib>PARK, Sang Mi</creatorcontrib><creatorcontrib>CHOI, Jae Bang</creatorcontrib><creatorcontrib>YOON, Bit Na Rae</creatorcontrib><title>RECOMBINANT VECTOR FOR EXPRESSING HETEROLOGOUS PROTEIN, AND METHOD FOR PRODUCING HAND-FOOT-AND-MOUTH VIRUS-LIKE PARTICLE STRUCTURAL PROTEIN BY USING SAME</title><description>The present invention relates to a recombinant vector for expressing a heterologous protein, and a method for producing a hand-foot-and-mouth virus-like particle structural protein by using same. The recombinant vector for expressing a heterologous protein, according to the present invention, has been identified to enable production of hand-foot-and-mouth disease virus-like particles in a high yield by mass-producing of structural proteins and controlling the expression level of 3CD for P1 precursor cleavage, and enables low-cost production of an antigenic protein that is structurally accurate. Therefore, the recombinant vector for expressing a heterologous protein and hand-foot-and-mouth virus-like particles produced using same, according to the present invention, can be variously used in the field of prevention and treatment of hand-foot-and-mouth disease. La présente invention concerne un vecteur recombiné pour l'expression d'une protéine hétérologue, ainsi qu'un procédé de production d'une protéine structurelle de particule du type viral de la fièvre aphteuse à l'aide de ce vecteur. Le vecteur recombiné pour l'expression d'une protéine hétérologue, selon la présente invention, a été identifié pour permettre la production de particules du type viral de la fièvre aphteuse avec un rendement élevé en produisant en masse des protéines structurelles et en maîtrisant le niveau d'expression de la 3CD pour le clivage du précurseur P1, et permet la production à faible coût d'une protéine antigénique structurellement exacte. Par conséquent, le vecteur recombiné pour l'expression d'une protéine hétérologue et les particules du type viral de la fièvre aphteuse produites à l'aide de ce vecteur, selon la présente invention, peuvent être utilisés de diverses manières dans le domaine de la prévention et du traitement de la fièvre aphteuse. 본 발명은 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용한 수족구 바이러스 유사입자 구조 단백질의 생산 방법에 관한 것이다. 본 발명에 따른 외래 단백질 발현을 위한 재조합 벡터는 구조단백질의 대량 생산과 P1 전구체의 절단을 위한 3CD 발현량을 조절함으로써 높은 수율로 수족구병 바이러스 유사입자 생산이 가능함을 확인하였으며, 정확한 구조의 항원 단백질을 저비용으로 생산할 수 있다는 장점이 있다. 따라서 본 발명에 다른 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용하여 생산된 수족구 바이러스 유사입자는 수족구병의 예방 및 치료 분야에서 다양하게 활용될 수 있다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjb0KwkAQhNNYiPoOC7YGjAranpeNOUxuw96eP1UIclaigr6Mb2sM2lsMs8x87PSjF6Omcm2ssgI71EIMWSs8VIzOGbuBHAWZCtqQd1AxCRo7AWVTKFFySju-zVOvO7xt4oxI4s9Rkpccdoa9iwuzRagUi9EFghP2Wjyr4vcU1kfw3aRTJQ6j3rm5PMLo64NonKHoPA73Wx0e9-YUruFZ72k2nS2SZLlczVUy_496AyAqRJc</recordid><startdate>20240606</startdate><enddate>20240606</enddate><creator>OH, Mi Kyoung</creator><creator>OH, Jong Min</creator><creator>HAN, Beom Ku</creator><creator>KIM, Hyun Il</creator><creator>LEE, Ji Hoon</creator><creator>WOO, Soo Dong</creator><creator>KIM, Hyun Soo</creator><creator>PARK, Sang Mi</creator><creator>CHOI, Jae Bang</creator><creator>YOON, Bit Na Rae</creator><scope>EVB</scope></search><sort><creationdate>20240606</creationdate><title>RECOMBINANT VECTOR FOR EXPRESSING HETEROLOGOUS PROTEIN, AND METHOD FOR PRODUCING HAND-FOOT-AND-MOUTH VIRUS-LIKE PARTICLE STRUCTURAL PROTEIN BY USING SAME</title><author>OH, Mi Kyoung ; OH, Jong Min ; HAN, Beom Ku ; KIM, Hyun Il ; LEE, Ji Hoon ; WOO, Soo Dong ; KIM, Hyun Soo ; PARK, Sang Mi ; CHOI, Jae Bang ; YOON, Bit Na Rae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2024117783A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; kor</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>OH, Mi Kyoung</creatorcontrib><creatorcontrib>OH, Jong Min</creatorcontrib><creatorcontrib>HAN, Beom Ku</creatorcontrib><creatorcontrib>KIM, Hyun Il</creatorcontrib><creatorcontrib>LEE, Ji Hoon</creatorcontrib><creatorcontrib>WOO, Soo Dong</creatorcontrib><creatorcontrib>KIM, Hyun Soo</creatorcontrib><creatorcontrib>PARK, Sang Mi</creatorcontrib><creatorcontrib>CHOI, Jae Bang</creatorcontrib><creatorcontrib>YOON, Bit Na Rae</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>OH, Mi Kyoung</au><au>OH, Jong Min</au><au>HAN, Beom Ku</au><au>KIM, Hyun Il</au><au>LEE, Ji Hoon</au><au>WOO, Soo Dong</au><au>KIM, Hyun Soo</au><au>PARK, Sang Mi</au><au>CHOI, Jae Bang</au><au>YOON, Bit Na Rae</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>RECOMBINANT VECTOR FOR EXPRESSING HETEROLOGOUS PROTEIN, AND METHOD FOR PRODUCING HAND-FOOT-AND-MOUTH VIRUS-LIKE PARTICLE STRUCTURAL PROTEIN BY USING SAME</title><date>2024-06-06</date><risdate>2024</risdate><abstract>The present invention relates to a recombinant vector for expressing a heterologous protein, and a method for producing a hand-foot-and-mouth virus-like particle structural protein by using same. The recombinant vector for expressing a heterologous protein, according to the present invention, has been identified to enable production of hand-foot-and-mouth disease virus-like particles in a high yield by mass-producing of structural proteins and controlling the expression level of 3CD for P1 precursor cleavage, and enables low-cost production of an antigenic protein that is structurally accurate. Therefore, the recombinant vector for expressing a heterologous protein and hand-foot-and-mouth virus-like particles produced using same, according to the present invention, can be variously used in the field of prevention and treatment of hand-foot-and-mouth disease. La présente invention concerne un vecteur recombiné pour l'expression d'une protéine hétérologue, ainsi qu'un procédé de production d'une protéine structurelle de particule du type viral de la fièvre aphteuse à l'aide de ce vecteur. Le vecteur recombiné pour l'expression d'une protéine hétérologue, selon la présente invention, a été identifié pour permettre la production de particules du type viral de la fièvre aphteuse avec un rendement élevé en produisant en masse des protéines structurelles et en maîtrisant le niveau d'expression de la 3CD pour le clivage du précurseur P1, et permet la production à faible coût d'une protéine antigénique structurellement exacte. Par conséquent, le vecteur recombiné pour l'expression d'une protéine hétérologue et les particules du type viral de la fièvre aphteuse produites à l'aide de ce vecteur, selon la présente invention, peuvent être utilisés de diverses manières dans le domaine de la prévention et du traitement de la fièvre aphteuse. 본 발명은 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용한 수족구 바이러스 유사입자 구조 단백질의 생산 방법에 관한 것이다. 본 발명에 따른 외래 단백질 발현을 위한 재조합 벡터는 구조단백질의 대량 생산과 P1 전구체의 절단을 위한 3CD 발현량을 조절함으로써 높은 수율로 수족구병 바이러스 유사입자 생산이 가능함을 확인하였으며, 정확한 구조의 항원 단백질을 저비용으로 생산할 수 있다는 장점이 있다. 따라서 본 발명에 다른 외래 단백질 발현을 위한 재조합 벡터 및 이를 이용하여 생산된 수족구 바이러스 유사입자는 수족구병의 예방 및 치료 분야에서 다양하게 활용될 수 있다.</abstract><oa>free_for_read</oa></addata></record>
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subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
title	RECOMBINANT VECTOR FOR EXPRESSING HETEROLOGOUS PROTEIN, AND METHOD FOR PRODUCING HAND-FOOT-AND-MOUTH VIRUS-LIKE PARTICLE STRUCTURAL PROTEIN BY USING SAME
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