NUCLEIC ACID ELUTION LIQUID AND NUCLEIC ACID ELUTION METHOD USING SAID NUCLEIC ACID ELUTION LIQUID

The present invention improves the recovery rate of nucleic acid bonded to a nucleic acid capturing carrier, and recovers the nucleic acid at a high concentration. In the present invention, nucleic acid bonded to a nucleic acid capturing carrier is recovered by using a nucleic acid elution liquid co...

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Hauptverfasser: AKIMOTO Kaoru, MATSUMOTO Yuki, FUJIOKA Michiru
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Sprache:eng ; fre ; jpn
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creator AKIMOTO Kaoru
MATSUMOTO Yuki
FUJIOKA Michiru
description The present invention improves the recovery rate of nucleic acid bonded to a nucleic acid capturing carrier, and recovers the nucleic acid at a high concentration. In the present invention, nucleic acid bonded to a nucleic acid capturing carrier is recovered by using a nucleic acid elution liquid containing: a polar solvent for dissolving the nucleic acid; and a hydrophobic solution that undergoes liquid-liquid layer separation with respect to the polar solvent. La présente invention améliore le taux de récupération de l'acide nucléique lié à un support de capture de l'acide nucléique et récupère l'acide nucléique à une concentration élevée. Dans la présente invention, l'acide nucléique lié à un support de capture d'acide nucléique est récupéré en utilisant un liquide d'élution d'acide nucléique contenant : un solvant polaire pour dissoudre l'acide nucléique ; et une solution hydrophobe subissant une séparation de couche liquide-liquide par rapport au solvant polaire. 核酸捕捉用担体に結合した核酸の回収率を向上するとともに高濃度で回収する。核酸を溶解する極性溶媒と、当該極性溶媒と液液層分離する疎水性溶液とを含む核酸溶離液を使用して核酸捕捉用担体に結合した核酸を回収する。
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title NUCLEIC ACID ELUTION LIQUID AND NUCLEIC ACID ELUTION METHOD USING SAID NUCLEIC ACID ELUTION LIQUID
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