METHOD AND REAGENT FOR NUCLEIC ACID EXTRACTION AND PURIFICATION USING POROUS NANOMATERIAL

A method for extracting nucleic acids from a biological sample (such as plasma) using nanopores in a porous material. The method induces nucleic acids into nanopores of the porous material to achieve separation and purification of nucleic acids. The method also has the advantage that the bound nucle...

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Hauptverfasser:	JIA, Xinye, DING, Qiutao, MENG, Xin, SHI, Xiaoyu, CHEN, Chengtong, HUANG, Yuchen, LIU, Jun, ZHANG, Qionglu, CHENG, Yanchao
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for extracting nucleic acids from a biological sample (such as plasma) using nanopores in a porous material. The method induces nucleic acids into nanopores of the porous material to achieve separation and purification of nucleic acids. The method also has the advantage that the bound nucleic acids will not separate from the pores under separation and cleaning conditions, and thus has good application prospects. The material containing nanopores can have added paramagnetic cores (for example, magnetic microspheres of ferroferric oxide) and be applied to a full-automatic nucleic acid extraction workstation to improve nucleic acid extraction efficiency. L'invention concerne également un procédé d'extraction d'acides nucléiques à partir d'un échantillon biologique (tel qu'un plasma) à l'aide de nanopores dans un matériau poreux. Le procédé induit des acides nucléiques dans des nanopores du matériau poreux pour obtenir une séparation et une purification d'acides nucléiques. Le procédé présente également