PROTECTING OLIGONUCLEOTIDES FOR CRISPR GUIDE RNA
Protecting oligonucleotides are provided. Protecting oligonucleotides with 5' and/or 3' conjugated moieties are provided. Protecting oligonucleotides with chemical modifications are provided. Methods of using the protecting oligonucleotides for genome editing with a CRISPR nuclease and kit...
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| creator | SONTHEIMER, Erik Joseph ZHANG, Han KHVOROVA, Anastasia WATTS, Jonathan Kenneth |
| description | Protecting oligonucleotides are provided. Protecting oligonucleotides with 5' and/or 3' conjugated moieties are provided. Protecting oligonucleotides with chemical modifications are provided. Methods of using the protecting oligonucleotides for genome editing with a CRISPR nuclease and kits for performing the same are also provided. Wherein a protecting oligonucleotide comprises: (a) a sequence that is complementary to a crRNA; and (b) at least one chemically modified nucleotide.
L'invention concerne des oligonucléotides protecteurs. L'invention concerne des oligonucléotides protecteurs avec des fractions conjuguées 5' et/ou 3'. L'invention concerne également des oligonucléotides protecteurs à modifications chimiques. L'invention concerne en outre des procédés d'utilisation des oligonucléotides protecteurs pour l'édition génomique avec une nucléase CRISPR et des kits pour leur mise en oeuvre. Un oligonucléotide protecteur comprend : (a) une séquence qui est complémentaire d'un ARNcr; et (b) au moins un nucléotide chimiquement modifié. |
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L'invention concerne des oligonucléotides protecteurs. L'invention concerne des oligonucléotides protecteurs avec des fractions conjuguées 5' et/ou 3'. L'invention concerne également des oligonucléotides protecteurs à modifications chimiques. L'invention concerne en outre des procédés d'utilisation des oligonucléotides protecteurs pour l'édition génomique avec une nucléase CRISPR et des kits pour leur mise en oeuvre. Un oligonucléotide protecteur comprend : (a) une séquence qui est complémentaire d'un ARNcr; et (b) au moins un nucléotide chimiquement modifié.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231102&DB=EPODOC&CC=WO&NR=2023212327A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231102&DB=EPODOC&CC=WO&NR=2023212327A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SONTHEIMER, Erik Joseph</creatorcontrib><creatorcontrib>ZHANG, Han</creatorcontrib><creatorcontrib>KHVOROVA, Anastasia</creatorcontrib><creatorcontrib>WATTS, Jonathan Kenneth</creatorcontrib><title>PROTECTING OLIGONUCLEOTIDES FOR CRISPR GUIDE RNA</title><description>Protecting oligonucleotides are provided. Protecting oligonucleotides with 5' and/or 3' conjugated moieties are provided. Protecting oligonucleotides with chemical modifications are provided. Methods of using the protecting oligonucleotides for genome editing with a CRISPR nuclease and kits for performing the same are also provided. Wherein a protecting oligonucleotide comprises: (a) a sequence that is complementary to a crRNA; and (b) at least one chemically modified nucleotide.
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L'invention concerne des oligonucléotides protecteurs. L'invention concerne des oligonucléotides protecteurs avec des fractions conjuguées 5' et/ou 3'. L'invention concerne également des oligonucléotides protecteurs à modifications chimiques. L'invention concerne en outre des procédés d'utilisation des oligonucléotides protecteurs pour l'édition génomique avec une nucléase CRISPR et des kits pour leur mise en oeuvre. Un oligonucléotide protecteur comprend : (a) une séquence qui est complémentaire d'un ARNcr; et (b) au moins un nucléotide chimiquement modifié.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PROTECTING OLIGONUCLEOTIDES FOR CRISPR GUIDE RNA |
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