HIGH EFFICIENCY PURIFICATION OF DIVERGENT AAV SEROTYPES USING AAVX AFFINITY CHROMATOGRAPHY
Disclosed herein are optimized methods of high efficiency purification of adeno- associated virus (AAV) particles, comprising a step of binding one or more AAV particles with a volume of chromatography resin medium comprising at least one ligand possessing a pan-AAV affinity. The optimized methods a...
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| creator | VANDENBERGHE, Luk WAGERS, Amy J FLOREA, Michael |
| description | Disclosed herein are optimized methods of high efficiency purification of adeno- associated virus (AAV) particles, comprising a step of binding one or more AAV particles with a volume of chromatography resin medium comprising at least one ligand possessing a pan-AAV affinity. The optimized methods are capable of providing purified AAV particles comprising a wide diversity of AAV serotypes without the need to further optimize for a given AAV serotype.
La présente invention concerne des procédés optimisés de purification à haut rendement de particules de virus adéno-associés (AAV), comprenant une étape de liaison d'une ou plusieurs particules AAV avec un volume de milieu de résine de chromatographie comprenant au moins un ligand possédant une affinité pan-AAV. Les procédés optimisés peuvent permettre d'obtenir des particules AAV purifiées comprenant une grande diversité de sérotypes AAV sans qu'il soit nécessaire de les optimiser davantage pour un sérotype AAV donné. |
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La présente invention concerne des procédés optimisés de purification à haut rendement de particules de virus adéno-associés (AAV), comprenant une étape de liaison d'une ou plusieurs particules AAV avec un volume de milieu de résine de chromatographie comprenant au moins un ligand possédant une affinité pan-AAV. Les procédés optimisés peuvent permettre d'obtenir des particules AAV purifiées comprenant une grande diversité de sérotypes AAV sans qu'il soit nécessaire de les optimiser davantage pour un sérotype AAV donné.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231005&DB=EPODOC&CC=WO&NR=2023191827A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231005&DB=EPODOC&CC=WO&NR=2023191827A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VANDENBERGHE, Luk</creatorcontrib><creatorcontrib>WAGERS, Amy J</creatorcontrib><creatorcontrib>FLOREA, Michael</creatorcontrib><title>HIGH EFFICIENCY PURIFICATION OF DIVERGENT AAV SEROTYPES USING AAVX AFFINITY CHROMATOGRAPHY</title><description>Disclosed herein are optimized methods of high efficiency purification of adeno- associated virus (AAV) particles, comprising a step of binding one or more AAV particles with a volume of chromatography resin medium comprising at least one ligand possessing a pan-AAV affinity. The optimized methods are capable of providing purified AAV particles comprising a wide diversity of AAV serotypes without the need to further optimize for a given AAV serotype.
La présente invention concerne des procédés optimisés de purification à haut rendement de particules de virus adéno-associés (AAV), comprenant une étape de liaison d'une ou plusieurs particules AAV avec un volume de milieu de résine de chromatographie comprenant au moins un ligand possédant une affinité pan-AAV. Les procédés optimisés peuvent permettre d'obtenir des particules AAV purifiées comprenant une grande diversité de sérotypes AAV sans qu'il soit nécessaire de les optimiser davantage pour un sérotype AAV donné.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi7EKwjAURbM4iPoPD5wF2w7q-IgvyRtMQppW41KKxEm0UP8fK_gBTpdzOHcuroa1AVKKJZOVCXwTeAKM7Cw4BUduKWiyERBbqCm4mDzV0NRs9dddAKe35ZhAmuBOGJ0O6E1aitm9f4x59duFWCuK0mzy8OryOPS3_Mzv7uzKbVkVh2Jf7rCo_qs-PMQzFA</recordid><startdate>20231005</startdate><enddate>20231005</enddate><creator>VANDENBERGHE, Luk</creator><creator>WAGERS, Amy J</creator><creator>FLOREA, Michael</creator><scope>EVB</scope></search><sort><creationdate>20231005</creationdate><title>HIGH EFFICIENCY PURIFICATION OF DIVERGENT AAV SEROTYPES USING AAVX AFFINITY CHROMATOGRAPHY</title><author>VANDENBERGHE, Luk ; WAGERS, Amy J ; FLOREA, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2023191827A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>VANDENBERGHE, Luk</creatorcontrib><creatorcontrib>WAGERS, Amy J</creatorcontrib><creatorcontrib>FLOREA, Michael</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VANDENBERGHE, Luk</au><au>WAGERS, Amy J</au><au>FLOREA, Michael</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>HIGH EFFICIENCY PURIFICATION OF DIVERGENT AAV SEROTYPES USING AAVX AFFINITY CHROMATOGRAPHY</title><date>2023-10-05</date><risdate>2023</risdate><abstract>Disclosed herein are optimized methods of high efficiency purification of adeno- associated virus (AAV) particles, comprising a step of binding one or more AAV particles with a volume of chromatography resin medium comprising at least one ligand possessing a pan-AAV affinity. The optimized methods are capable of providing purified AAV particles comprising a wide diversity of AAV serotypes without the need to further optimize for a given AAV serotype.
La présente invention concerne des procédés optimisés de purification à haut rendement de particules de virus adéno-associés (AAV), comprenant une étape de liaison d'une ou plusieurs particules AAV avec un volume de milieu de résine de chromatographie comprenant au moins un ligand possédant une affinité pan-AAV. Les procédés optimisés peuvent permettre d'obtenir des particules AAV purifiées comprenant une grande diversité de sérotypes AAV sans qu'il soit nécessaire de les optimiser davantage pour un sérotype AAV donné.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | HIGH EFFICIENCY PURIFICATION OF DIVERGENT AAV SEROTYPES USING AAVX AFFINITY CHROMATOGRAPHY |
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