BST POLYMERASE VARIANTS
The present disclosure describes Bst polymerase variants which exhibit DNA-dependent DNA polymerase and reverse transcriptase activity, and methods of use thereof. The Bst polymerase variants of the disclosure may be combined with a separate enzyme having reverse transcriptase activity to amplify ta...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | THROCKMORTON, Kurt CHRISTIAN, Thomas MEAD, David NEEL, Brandon CHANDER, Yogesh |
| description | The present disclosure describes Bst polymerase variants which exhibit DNA-dependent DNA polymerase and reverse transcriptase activity, and methods of use thereof. The Bst polymerase variants of the disclosure may be combined with a separate enzyme having reverse transcriptase activity to amplify target RNA sequences; however, the addition of a separate enzyme having reverse transcriptase activity is not necessary for the successful amplification of RNA targets using the Bst polymerase variants of the disclosure. Target DNA sequences may also be amplified using Bst polymerase variants of the disclosure.
La présente divulgation concerne des variants de Bst polymérase qui présentent une activité ADN polymérase dépendante de l'ADN et une activité de transcriptase inverse, et leurs méthodes d'utilisation. Les variants de Bst polymérase de la divulgation peuvent être combinées avec une enzyme séparée présentant une activité de transcriptase inverse pour amplifier des séquences d'ARN cibles ; cependant, l'ajout d'une enzyme séparée présentant une activité de transcriptase inverse n'est pas nécessaire pour l'amplification réussie de cibles d'ARN à l'aide des variants de Bst polymérase de la divulgation. Des séquences d'ADN cibles peuvent également être amplifiées à l'aide de variants de Bst polymérase de la divulgation. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2023172988A3</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2023172988A3</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2023172988A33</originalsourceid><addsrcrecordid>eNrjZBB3Cg5RCPD3ifR1DXIMdlUIcwzydPQLCeZhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGRsaG5kaWFhaOxsbEqQIAY2ggmA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>BST POLYMERASE VARIANTS</title><source>esp@cenet</source><creator>THROCKMORTON, Kurt ; CHRISTIAN, Thomas ; MEAD, David ; NEEL, Brandon ; CHANDER, Yogesh</creator><creatorcontrib>THROCKMORTON, Kurt ; CHRISTIAN, Thomas ; MEAD, David ; NEEL, Brandon ; CHANDER, Yogesh</creatorcontrib><description>The present disclosure describes Bst polymerase variants which exhibit DNA-dependent DNA polymerase and reverse transcriptase activity, and methods of use thereof. The Bst polymerase variants of the disclosure may be combined with a separate enzyme having reverse transcriptase activity to amplify target RNA sequences; however, the addition of a separate enzyme having reverse transcriptase activity is not necessary for the successful amplification of RNA targets using the Bst polymerase variants of the disclosure. Target DNA sequences may also be amplified using Bst polymerase variants of the disclosure.
La présente divulgation concerne des variants de Bst polymérase qui présentent une activité ADN polymérase dépendante de l'ADN et une activité de transcriptase inverse, et leurs méthodes d'utilisation. Les variants de Bst polymérase de la divulgation peuvent être combinées avec une enzyme séparée présentant une activité de transcriptase inverse pour amplifier des séquences d'ARN cibles ; cependant, l'ajout d'une enzyme séparée présentant une activité de transcriptase inverse n'est pas nécessaire pour l'amplification réussie de cibles d'ARN à l'aide des variants de Bst polymérase de la divulgation. Des séquences d'ADN cibles peuvent également être amplifiées à l'aide de variants de Bst polymérase de la divulgation.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231012&DB=EPODOC&CC=WO&NR=2023172988A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231012&DB=EPODOC&CC=WO&NR=2023172988A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>THROCKMORTON, Kurt</creatorcontrib><creatorcontrib>CHRISTIAN, Thomas</creatorcontrib><creatorcontrib>MEAD, David</creatorcontrib><creatorcontrib>NEEL, Brandon</creatorcontrib><creatorcontrib>CHANDER, Yogesh</creatorcontrib><title>BST POLYMERASE VARIANTS</title><description>The present disclosure describes Bst polymerase variants which exhibit DNA-dependent DNA polymerase and reverse transcriptase activity, and methods of use thereof. The Bst polymerase variants of the disclosure may be combined with a separate enzyme having reverse transcriptase activity to amplify target RNA sequences; however, the addition of a separate enzyme having reverse transcriptase activity is not necessary for the successful amplification of RNA targets using the Bst polymerase variants of the disclosure. Target DNA sequences may also be amplified using Bst polymerase variants of the disclosure.
La présente divulgation concerne des variants de Bst polymérase qui présentent une activité ADN polymérase dépendante de l'ADN et une activité de transcriptase inverse, et leurs méthodes d'utilisation. Les variants de Bst polymérase de la divulgation peuvent être combinées avec une enzyme séparée présentant une activité de transcriptase inverse pour amplifier des séquences d'ARN cibles ; cependant, l'ajout d'une enzyme séparée présentant une activité de transcriptase inverse n'est pas nécessaire pour l'amplification réussie de cibles d'ARN à l'aide des variants de Bst polymérase de la divulgation. Des séquences d'ADN cibles peuvent également être amplifiées à l'aide de variants de Bst polymérase de la divulgation.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZBB3Cg5RCPD3ifR1DXIMdlUIcwzydPQLCeZhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGRsaG5kaWFhaOxsbEqQIAY2ggmA</recordid><startdate>20231012</startdate><enddate>20231012</enddate><creator>THROCKMORTON, Kurt</creator><creator>CHRISTIAN, Thomas</creator><creator>MEAD, David</creator><creator>NEEL, Brandon</creator><creator>CHANDER, Yogesh</creator><scope>EVB</scope></search><sort><creationdate>20231012</creationdate><title>BST POLYMERASE VARIANTS</title><author>THROCKMORTON, Kurt ; CHRISTIAN, Thomas ; MEAD, David ; NEEL, Brandon ; CHANDER, Yogesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2023172988A33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>THROCKMORTON, Kurt</creatorcontrib><creatorcontrib>CHRISTIAN, Thomas</creatorcontrib><creatorcontrib>MEAD, David</creatorcontrib><creatorcontrib>NEEL, Brandon</creatorcontrib><creatorcontrib>CHANDER, Yogesh</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>THROCKMORTON, Kurt</au><au>CHRISTIAN, Thomas</au><au>MEAD, David</au><au>NEEL, Brandon</au><au>CHANDER, Yogesh</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>BST POLYMERASE VARIANTS</title><date>2023-10-12</date><risdate>2023</risdate><abstract>The present disclosure describes Bst polymerase variants which exhibit DNA-dependent DNA polymerase and reverse transcriptase activity, and methods of use thereof. The Bst polymerase variants of the disclosure may be combined with a separate enzyme having reverse transcriptase activity to amplify target RNA sequences; however, the addition of a separate enzyme having reverse transcriptase activity is not necessary for the successful amplification of RNA targets using the Bst polymerase variants of the disclosure. Target DNA sequences may also be amplified using Bst polymerase variants of the disclosure.
La présente divulgation concerne des variants de Bst polymérase qui présentent une activité ADN polymérase dépendante de l'ADN et une activité de transcriptase inverse, et leurs méthodes d'utilisation. Les variants de Bst polymérase de la divulgation peuvent être combinées avec une enzyme séparée présentant une activité de transcriptase inverse pour amplifier des séquences d'ARN cibles ; cependant, l'ajout d'une enzyme séparée présentant une activité de transcriptase inverse n'est pas nécessaire pour l'amplification réussie de cibles d'ARN à l'aide des variants de Bst polymérase de la divulgation. Des séquences d'ADN cibles peuvent également être amplifiées à l'aide de variants de Bst polymérase de la divulgation.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO2023172988A3 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | BST POLYMERASE VARIANTS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T09%3A37%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=THROCKMORTON,%20Kurt&rft.date=2023-10-12&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO2023172988A3%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |