COMPOSITION AND METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES
The invention relates to a mastermix composition for the amplification of nucleic acid sequences, which is intended to be mixed with a biological sample containing one or more target nucleic acid sequences and with primers specific for the target nucleic acid sequence(s), and fluorescent probes for...
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| creator | MURLYKINA, Maryna PANNETIER, Christophe CROZZOLO, Julien LE BERRE, Maël VILLA, Francesco |
| description | The invention relates to a mastermix composition for the amplification of nucleic acid sequences, which is intended to be mixed with a biological sample containing one or more target nucleic acid sequences and with primers specific for the target nucleic acid sequence(s), and fluorescent probes for revealing an amplification of the target nucleic acid sequence(s), the composition comprising reagents and enzymes for nucleic acid application, characterized in that the composition also comprises a polymerizable reagent, which polymerizes under the influence of a stimulation chosen from a heat stimulation and a light stimulation, so as to form a hydrogel which locally encapsulates each target nucleic acid sequence when said composition is exposed to said stimulation. The invention also relates to a method using said composition.
L'invention vise une composition de mélange maître pour l'amplification de séquences d'acides nucléiques, destinée à être mélangée à un échantillon biologique contenant une ou plusieurs séquences d'acides nucléiques cibles et à des amorces spécifiques de la ou des séquences d'acides nucléiques cibles, ainsi que des sondes fluorescentes pour révéler une amplification de la ou des séquences d'acides nucléiques cibles, la composition comprenant des réactifs et des enzymes d'amplification d'acides nucléiques, caractérisé en ce que la composition comprend en outre un réactif polymérisable, lequel polymérise sous l'influence d'une stimulation choisie parmi une stimulation thermique et une stimulation lumineuse, de sorte à former un hydrogel encapsulant localement chaque séquence d'acides nucléiques cible lorsque ladite composition est exposée à ladite stimulation. L'invention vise aussi un procédé utilisant cette composition. |
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L'invention vise une composition de mélange maître pour l'amplification de séquences d'acides nucléiques, destinée à être mélangée à un échantillon biologique contenant une ou plusieurs séquences d'acides nucléiques cibles et à des amorces spécifiques de la ou des séquences d'acides nucléiques cibles, ainsi que des sondes fluorescentes pour révéler une amplification de la ou des séquences d'acides nucléiques cibles, la composition comprenant des réactifs et des enzymes d'amplification d'acides nucléiques, caractérisé en ce que la composition comprend en outre un réactif polymérisable, lequel polymérise sous l'influence d'une stimulation choisie parmi une stimulation thermique et une stimulation lumineuse, de sorte à former un hydrogel encapsulant localement chaque séquence d'acides nucléiques cible lorsque ladite composition est exposée à ladite stimulation. L'invention vise aussi un procédé utilisant cette composition.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230629&DB=EPODOC&CC=WO&NR=2023118693A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230629&DB=EPODOC&CC=WO&NR=2023118693A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MURLYKINA, Maryna</creatorcontrib><creatorcontrib>PANNETIER, Christophe</creatorcontrib><creatorcontrib>CROZZOLO, Julien</creatorcontrib><creatorcontrib>LE BERRE, Maël</creatorcontrib><creatorcontrib>VILLA, Francesco</creatorcontrib><title>COMPOSITION AND METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES</title><description>The invention relates to a mastermix composition for the amplification of nucleic acid sequences, which is intended to be mixed with a biological sample containing one or more target nucleic acid sequences and with primers specific for the target nucleic acid sequence(s), and fluorescent probes for revealing an amplification of the target nucleic acid sequence(s), the composition comprising reagents and enzymes for nucleic acid application, characterized in that the composition also comprises a polymerizable reagent, which polymerizes under the influence of a stimulation chosen from a heat stimulation and a light stimulation, so as to form a hydrogel which locally encapsulates each target nucleic acid sequence when said composition is exposed to said stimulation. The invention also relates to a method using said composition.
L'invention vise une composition de mélange maître pour l'amplification de séquences d'acides nucléiques, destinée à être mélangée à un échantillon biologique contenant une ou plusieurs séquences d'acides nucléiques cibles et à des amorces spécifiques de la ou des séquences d'acides nucléiques cibles, ainsi que des sondes fluorescentes pour révéler une amplification de la ou des séquences d'acides nucléiques cibles, la composition comprenant des réactifs et des enzymes d'amplification d'acides nucléiques, caractérisé en ce que la composition comprend en outre un réactif polymérisable, lequel polymérise sous l'influence d'une stimulation choisie parmi une stimulation thermique et une stimulation lumineuse, de sorte à former un hydrogel encapsulant localement chaque séquence d'acides nucléiques cible lorsque ladite composition est exposée à ladite stimulation. L'invention vise aussi un procédé utilisant cette composition.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLBx9vcN8A_2DPH091Nw9HNR8HUN8fB3UXDzD1Jw9A3w8XSL9PRzV_ALdfZx9XRWcHT2dFEIdg0MdfVzdg3mYWBNS8wpTuWF0twMym6uIc4euqkF-fGpxQWJyal5qSXx4f5GBkbGhoYWZpbGjobGxKkCAKrhKng</recordid><startdate>20230629</startdate><enddate>20230629</enddate><creator>MURLYKINA, Maryna</creator><creator>PANNETIER, Christophe</creator><creator>CROZZOLO, Julien</creator><creator>LE BERRE, Maël</creator><creator>VILLA, Francesco</creator><scope>EVB</scope></search><sort><creationdate>20230629</creationdate><title>COMPOSITION AND METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES</title><author>MURLYKINA, Maryna ; PANNETIER, Christophe ; CROZZOLO, Julien ; LE BERRE, Maël ; VILLA, Francesco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2023118693A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MURLYKINA, Maryna</creatorcontrib><creatorcontrib>PANNETIER, Christophe</creatorcontrib><creatorcontrib>CROZZOLO, Julien</creatorcontrib><creatorcontrib>LE BERRE, Maël</creatorcontrib><creatorcontrib>VILLA, Francesco</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MURLYKINA, Maryna</au><au>PANNETIER, Christophe</au><au>CROZZOLO, Julien</au><au>LE BERRE, Maël</au><au>VILLA, Francesco</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>COMPOSITION AND METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES</title><date>2023-06-29</date><risdate>2023</risdate><abstract>The invention relates to a mastermix composition for the amplification of nucleic acid sequences, which is intended to be mixed with a biological sample containing one or more target nucleic acid sequences and with primers specific for the target nucleic acid sequence(s), and fluorescent probes for revealing an amplification of the target nucleic acid sequence(s), the composition comprising reagents and enzymes for nucleic acid application, characterized in that the composition also comprises a polymerizable reagent, which polymerizes under the influence of a stimulation chosen from a heat stimulation and a light stimulation, so as to form a hydrogel which locally encapsulates each target nucleic acid sequence when said composition is exposed to said stimulation. The invention also relates to a method using said composition.
L'invention vise une composition de mélange maître pour l'amplification de séquences d'acides nucléiques, destinée à être mélangée à un échantillon biologique contenant une ou plusieurs séquences d'acides nucléiques cibles et à des amorces spécifiques de la ou des séquences d'acides nucléiques cibles, ainsi que des sondes fluorescentes pour révéler une amplification de la ou des séquences d'acides nucléiques cibles, la composition comprenant des réactifs et des enzymes d'amplification d'acides nucléiques, caractérisé en ce que la composition comprend en outre un réactif polymérisable, lequel polymérise sous l'influence d'une stimulation choisie parmi une stimulation thermique et une stimulation lumineuse, de sorte à former un hydrogel encapsulant localement chaque séquence d'acides nucléiques cible lorsque ladite composition est exposée à ladite stimulation. L'invention vise aussi un procédé utilisant cette composition.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | COMPOSITION AND METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCES |
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