METHOD FOR PREPARING RANDOM SGRNA LIBRARY OF TARGET SEQUENCE BY MEANS OF ENZYMATIC METHOD

Provided in the present invention is a method for preparing a random sgRNA library of a target sequence by means of an enzymatic method. The preparation method comprises the steps of: nicking a PAM region of the target sequence of sample DNA with a random nickase Nt.CviPII; linking the fragmented DN...

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Bibliographische Detailangaben
Hauptverfasser:	LIU, Qian, SUN, Rui, WANG, Man, SONG, Dongliang, HUANG, Cheng, CHEN, Jingjing, CAO, Zhen, HOU, Ce
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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