INCREASED TRANSFORMABILITY AND HAPLOID INDUCTION IN PLANTS

Provided herein are highly transformable maize plants, referred to as HI-NA plants, and methods of their production and use. A HI-NA plant, as disclosed herein, is homozygous for a loss-of-function mutant allele in the patatin-like phospholipase A2α (MATL) gene and at least heterozygous for one or m...

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Hauptverfasser: SKIBBE, David Stewart, KELLIHER, Timothy Joseph, NICHOLS, Jason, DELZER, Brent
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creator SKIBBE, David Stewart
KELLIHER, Timothy Joseph
NICHOLS, Jason
DELZER, Brent
description Provided herein are highly transformable maize plants, referred to as HI-NA plants, and methods of their production and use. A HI-NA plant, as disclosed herein, is homozygous for a loss-of-function mutant allele in the patatin-like phospholipase A2α (MATL) gene and at least heterozygous for one or more QTL and/or gene alleles that are responsible for increased haploid induction and/or transformation frequency in plants. A HI-NA plant, as disclosed herein, may also have a cytotype A background, which may render it highly transformable. Also provided are methods of producing HI-NA plants and methods of using a HI-NA plant for editing plant genomic DNA. La présente invention concerne des plantes de maïs hautement transformables, appelées plantes HI-NA, et leurs procédés de production et d'utilisation. Une plante HI-NA, telle que décrite selon l'invention, est homozygote pour un allèle mutant de perte de fonction dans le gène de phospholipase A2α du type patatine (MATL) et au moins hétérozygote pour au moins un QTL et/ou des allèles de gène qui sont responsables de l'augmentation de la fréquence d'induction et/ou de transformation d'haploïdes dans des plantes. Une plante HI-NA, telle que divulguée selon l'invention, peut également avoir un arrière-plan de type cytotype A, qui peut le rendre hautement transformable. L'invention concerne, en outre, des procédés de production de plantes HI-NA et des procédés d'utilisation d'une plante HI-NA pour l'édition d'ADN génomique végétal.
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A HI-NA plant, as disclosed herein, is homozygous for a loss-of-function mutant allele in the patatin-like phospholipase A2α (MATL) gene and at least heterozygous for one or more QTL and/or gene alleles that are responsible for increased haploid induction and/or transformation frequency in plants. A HI-NA plant, as disclosed herein, may also have a cytotype A background, which may render it highly transformable. Also provided are methods of producing HI-NA plants and methods of using a HI-NA plant for editing plant genomic DNA. La présente invention concerne des plantes de maïs hautement transformables, appelées plantes HI-NA, et leurs procédés de production et d'utilisation. 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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
ORGANIC CHEMISTRY
PEPTIDES
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
title INCREASED TRANSFORMABILITY AND HAPLOID INDUCTION IN PLANTS
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