INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS
The claimed invention discloses a complex of housekeeping genes and effector protein genes of Phytophthora investans which can be used as targets for inhibiting expression using the RNA interference (RNAi) method, which as a result ensures plant protection from the pathogen. In accordance with this...
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| creator | KALININA, Natalya Olegovna SOLOVEV, Andrey Gennadevich CHUENKO, Aleksandr Mihaylovich MARKIN, Nikolay Viktorovich TALYANSKIY, Mihail Emmanuilovich HROMOV, Andrey Vladimirovich SUPRUNOVA, Tatyana Pavlovna MOROZOV, Sergey Yurevich |
| description | The claimed invention discloses a complex of housekeeping genes and effector protein genes of Phytophthora investans which can be used as targets for inhibiting expression using the RNA interference (RNAi) method, which as a result ensures plant protection from the pathogen. In accordance with this invention, 24 target genes are proposed: 12 housekeeping genes and 12 effector protein genes, based on the combined sequences of which in vitro 8 variants of dsRNA are synthesized. Spraying potato plant leaves with an aqueous solution of a composition containing a complex of synthesized dsDNA results in inhibition of reproduction of the oomycete Phytophthora infestans.
La présente invention concerne un complexe de gènes à usage domestique et de gènes de protéines-effecteurs Phytophthora infestans, qui peuvent être utilisés en qualité de cibles pour inhiber l'expression selon le mécanisme ARN d'interférence (ARNi), ce qui permet de protéger des végétaux contre un pathogène. La présente invention concerne 24 gènes-cibles: 12 gènes domestiques et 12 gènes de protéines-effecteurs, 8 variantes d'ARN double brin étant synthétisées in vitro sur la base des séquences combinées de ces derniers. L'aspersion de feuilles de de plants de pomme de terre avec une solution aqueuse de la composition contenant un complexe des ARN double brin synthétisés entraîne une inhibition de la multiplications de l'oocmycète Phytophthora infestans.
В заявленном изобретении раскрыт комплекс генов домашнего хозяйства и генов белков-эффекторов Phytophthora infestans, которые могут быть использованы в качестве мишеней для ингибирования экспрессии по механизму РНК интерференции (РНКи), что в результате обеспечивает защиту растений от патогена. В соответствии с настоящим изобретением предложены 24 гена-мишени: 12 генов домашнего хозяйства и 12 генов белков-эффекторов, на основе скомбинированных последовательностей которых in vitro синтезированы 8 вариантов дцРНК. Опрыскивание листьев растений картофеля водным раствором композиции, содержащей комплекс синтезированных дцРНК, приводит к ингибированию размножения оомицета Phytophthora infestans. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2022146195A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2022146195A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2022146195A13</originalsourceid><addsrcrecordid>eNrjZLD39AtxDXJzDfL0c1dwCQ7yc1Rw8w9SCAjyD3F1DgEJBvg4-oUEKzi6O3r6BYcoBHhEhvgHeIR4-Af5B3sG8zCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-HB_IwMjI0MTM0NLU0dDY-JUAQByGyvx</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS</title><source>esp@cenet</source><creator>KALININA, Natalya Olegovna ; SOLOVEV, Andrey Gennadevich ; CHUENKO, Aleksandr Mihaylovich ; MARKIN, Nikolay Viktorovich ; TALYANSKIY, Mihail Emmanuilovich ; HROMOV, Andrey Vladimirovich ; SUPRUNOVA, Tatyana Pavlovna ; MOROZOV, Sergey Yurevich</creator><creatorcontrib>KALININA, Natalya Olegovna ; SOLOVEV, Andrey Gennadevich ; CHUENKO, Aleksandr Mihaylovich ; MARKIN, Nikolay Viktorovich ; TALYANSKIY, Mihail Emmanuilovich ; HROMOV, Andrey Vladimirovich ; SUPRUNOVA, Tatyana Pavlovna ; MOROZOV, Sergey Yurevich</creatorcontrib><description>The claimed invention discloses a complex of housekeeping genes and effector protein genes of Phytophthora investans which can be used as targets for inhibiting expression using the RNA interference (RNAi) method, which as a result ensures plant protection from the pathogen. In accordance with this invention, 24 target genes are proposed: 12 housekeeping genes and 12 effector protein genes, based on the combined sequences of which in vitro 8 variants of dsRNA are synthesized. Spraying potato plant leaves with an aqueous solution of a composition containing a complex of synthesized dsDNA results in inhibition of reproduction of the oomycete Phytophthora infestans.
La présente invention concerne un complexe de gènes à usage domestique et de gènes de protéines-effecteurs Phytophthora infestans, qui peuvent être utilisés en qualité de cibles pour inhiber l'expression selon le mécanisme ARN d'interférence (ARNi), ce qui permet de protéger des végétaux contre un pathogène. La présente invention concerne 24 gènes-cibles: 12 gènes domestiques et 12 gènes de protéines-effecteurs, 8 variantes d'ARN double brin étant synthétisées in vitro sur la base des séquences combinées de ces derniers. L'aspersion de feuilles de de plants de pomme de terre avec une solution aqueuse de la composition contenant un complexe des ARN double brin synthétisés entraîne une inhibition de la multiplications de l'oocmycète Phytophthora infestans.
В заявленном изобретении раскрыт комплекс генов домашнего хозяйства и генов белков-эффекторов Phytophthora infestans, которые могут быть использованы в качестве мишеней для ингибирования экспрессии по механизму РНК интерференции (РНКи), что в результате обеспечивает защиту растений от патогена. В соответствии с настоящим изобретением предложены 24 гена-мишени: 12 генов домашнего хозяйства и 12 генов белков-эффекторов, на основе скомбинированных последовательностей которых in vitro синтезированы 8 вариантов дцРНК. Опрыскивание листьев растений картофеля водным раствором композиции, содержащей комплекс синтезированных дцРНК, приводит к ингибированию размножения оомицета Phytophthora infestans.</description><language>eng ; fre ; rus</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220707&DB=EPODOC&CC=WO&NR=2022146195A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220707&DB=EPODOC&CC=WO&NR=2022146195A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KALININA, Natalya Olegovna</creatorcontrib><creatorcontrib>SOLOVEV, Andrey Gennadevich</creatorcontrib><creatorcontrib>CHUENKO, Aleksandr Mihaylovich</creatorcontrib><creatorcontrib>MARKIN, Nikolay Viktorovich</creatorcontrib><creatorcontrib>TALYANSKIY, Mihail Emmanuilovich</creatorcontrib><creatorcontrib>HROMOV, Andrey Vladimirovich</creatorcontrib><creatorcontrib>SUPRUNOVA, Tatyana Pavlovna</creatorcontrib><creatorcontrib>MOROZOV, Sergey Yurevich</creatorcontrib><title>INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS</title><description>The claimed invention discloses a complex of housekeeping genes and effector protein genes of Phytophthora investans which can be used as targets for inhibiting expression using the RNA interference (RNAi) method, which as a result ensures plant protection from the pathogen. In accordance with this invention, 24 target genes are proposed: 12 housekeeping genes and 12 effector protein genes, based on the combined sequences of which in vitro 8 variants of dsRNA are synthesized. Spraying potato plant leaves with an aqueous solution of a composition containing a complex of synthesized dsDNA results in inhibition of reproduction of the oomycete Phytophthora infestans.
La présente invention concerne un complexe de gènes à usage domestique et de gènes de protéines-effecteurs Phytophthora infestans, qui peuvent être utilisés en qualité de cibles pour inhiber l'expression selon le mécanisme ARN d'interférence (ARNi), ce qui permet de protéger des végétaux contre un pathogène. La présente invention concerne 24 gènes-cibles: 12 gènes domestiques et 12 gènes de protéines-effecteurs, 8 variantes d'ARN double brin étant synthétisées in vitro sur la base des séquences combinées de ces derniers. L'aspersion de feuilles de de plants de pomme de terre avec une solution aqueuse de la composition contenant un complexe des ARN double brin synthétisés entraîne une inhibition de la multiplications de l'oocmycète Phytophthora infestans.
В заявленном изобретении раскрыт комплекс генов домашнего хозяйства и генов белков-эффекторов Phytophthora infestans, которые могут быть использованы в качестве мишеней для ингибирования экспрессии по механизму РНК интерференции (РНКи), что в результате обеспечивает защиту растений от патогена. В соответствии с настоящим изобретением предложены 24 гена-мишени: 12 генов домашнего хозяйства и 12 генов белков-эффекторов, на основе скомбинированных последовательностей которых in vitro синтезированы 8 вариантов дцРНК. Опрыскивание листьев растений картофеля водным раствором композиции, содержащей комплекс синтезированных дцРНК, приводит к ингибированию размножения оомицета Phytophthora infestans.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD39AtxDXJzDfL0c1dwCQ7yc1Rw8w9SCAjyD3F1DgEJBvg4-oUEKzi6O3r6BYcoBHhEhvgHeIR4-Af5B3sG8zCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-HB_IwMjI0MTM0NLU0dDY-JUAQByGyvx</recordid><startdate>20220707</startdate><enddate>20220707</enddate><creator>KALININA, Natalya Olegovna</creator><creator>SOLOVEV, Andrey Gennadevich</creator><creator>CHUENKO, Aleksandr Mihaylovich</creator><creator>MARKIN, Nikolay Viktorovich</creator><creator>TALYANSKIY, Mihail Emmanuilovich</creator><creator>HROMOV, Andrey Vladimirovich</creator><creator>SUPRUNOVA, Tatyana Pavlovna</creator><creator>MOROZOV, Sergey Yurevich</creator><scope>EVB</scope></search><sort><creationdate>20220707</creationdate><title>INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS</title><author>KALININA, Natalya Olegovna ; SOLOVEV, Andrey Gennadevich ; CHUENKO, Aleksandr Mihaylovich ; MARKIN, Nikolay Viktorovich ; TALYANSKIY, Mihail Emmanuilovich ; HROMOV, Andrey Vladimirovich ; SUPRUNOVA, Tatyana Pavlovna ; MOROZOV, Sergey Yurevich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2022146195A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; rus</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KALININA, Natalya Olegovna</creatorcontrib><creatorcontrib>SOLOVEV, Andrey Gennadevich</creatorcontrib><creatorcontrib>CHUENKO, Aleksandr Mihaylovich</creatorcontrib><creatorcontrib>MARKIN, Nikolay Viktorovich</creatorcontrib><creatorcontrib>TALYANSKIY, Mihail Emmanuilovich</creatorcontrib><creatorcontrib>HROMOV, Andrey Vladimirovich</creatorcontrib><creatorcontrib>SUPRUNOVA, Tatyana Pavlovna</creatorcontrib><creatorcontrib>MOROZOV, Sergey Yurevich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KALININA, Natalya Olegovna</au><au>SOLOVEV, Andrey Gennadevich</au><au>CHUENKO, Aleksandr Mihaylovich</au><au>MARKIN, Nikolay Viktorovich</au><au>TALYANSKIY, Mihail Emmanuilovich</au><au>HROMOV, Andrey Vladimirovich</au><au>SUPRUNOVA, Tatyana Pavlovna</au><au>MOROZOV, Sergey Yurevich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS</title><date>2022-07-07</date><risdate>2022</risdate><abstract>The claimed invention discloses a complex of housekeeping genes and effector protein genes of Phytophthora investans which can be used as targets for inhibiting expression using the RNA interference (RNAi) method, which as a result ensures plant protection from the pathogen. In accordance with this invention, 24 target genes are proposed: 12 housekeeping genes and 12 effector protein genes, based on the combined sequences of which in vitro 8 variants of dsRNA are synthesized. Spraying potato plant leaves with an aqueous solution of a composition containing a complex of synthesized dsDNA results in inhibition of reproduction of the oomycete Phytophthora infestans.
La présente invention concerne un complexe de gènes à usage domestique et de gènes de protéines-effecteurs Phytophthora infestans, qui peuvent être utilisés en qualité de cibles pour inhiber l'expression selon le mécanisme ARN d'interférence (ARNi), ce qui permet de protéger des végétaux contre un pathogène. La présente invention concerne 24 gènes-cibles: 12 gènes domestiques et 12 gènes de protéines-effecteurs, 8 variantes d'ARN double brin étant synthétisées in vitro sur la base des séquences combinées de ces derniers. L'aspersion de feuilles de de plants de pomme de terre avec une solution aqueuse de la composition contenant un complexe des ARN double brin synthétisés entraîne une inhibition de la multiplications de l'oocmycète Phytophthora infestans.
В заявленном изобретении раскрыт комплекс генов домашнего хозяйства и генов белков-эффекторов Phytophthora infestans, которые могут быть использованы в качестве мишеней для ингибирования экспрессии по механизму РНК интерференции (РНКи), что в результате обеспечивает защиту растений от патогена. В соответствии с настоящим изобретением предложены 24 гена-мишени: 12 генов домашнего хозяйства и 12 генов белков-эффекторов, на основе скомбинированных последовательностей которых in vitro синтезированы 8 вариантов дцРНК. Опрыскивание листьев растений картофеля водным раствором композиции, содержащей комплекс синтезированных дцРНК, приводит к ингибированию размножения оомицета Phytophthora infestans.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | INTERFERING DSRNA FOR PROTECTING PLANTS AGAINST PHYTOPHTHOROSIS |
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