METHOD FOR CONSTRUCTING MULTIPLEX PCR LIBRARY FOR HIGH-THROUGHPUT TARGETED SEQUENCING
A method for constructing a multiplex PCR library for high-throughput targeted sequencing: first acquiring a targeted DNA product by means of a high-specificity multiplex PCR reaction, and then digesting with a specific endonuclease, such that a specific molecular barcode is produced at the tail end...
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| creator | ZHU, Jun WAKABAYASHI, Yoshi YANG, Chuxuan BAI, Bing ZHANG, Yongshu |
| description | A method for constructing a multiplex PCR library for high-throughput targeted sequencing: first acquiring a targeted DNA product by means of a high-specificity multiplex PCR reaction, and then digesting with a specific endonuclease, such that a specific molecular barcode is produced at the tail end of the PCR product; thus, the library construction process is more efficient, and the accuracy and sequencing depth of the obtained data are also ensured.
L'invention concerne un procédé de construction d'une bibliothèque PCR multiplex pour séquençage ciblé à haut débit comprenant les étapes suivantes : tout d'abord, l'acquisition d'un produit d'ADN ciblé au moyen d'une réaction PCR multiplex à haute spécificité, puis la digestion avec une endonucléase spécifique, de telle sorte qu'un code à barres moléculaire spécifique est produit au niveau de l'extrémité de queue du produit de PCR ; ainsi, le processus de construction de la bibliothèque est plus efficace et la précision et la profondeur de séquençage des donnée |
| format | Patent |
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L'invention concerne un procédé de construction d'une bibliothèque PCR multiplex pour séquençage ciblé à haut débit comprenant les étapes suivantes : tout d'abord, l'acquisition d'un produit d'ADN ciblé au moyen d'une réaction PCR multiplex à haute spécificité, puis la digestion avec une endonucléase spécifique, de telle sorte qu'un code à barres moléculaire spécifique est produit au niveau de l'extrémité de queue du produit de PCR ; ainsi, le processus de construction de la bibliothèque est plus efficace et la précision et la profondeur de séquençage des donnée</description><language>chi ; eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220707&DB=EPODOC&CC=WO&NR=2022144003A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220707&DB=EPODOC&CC=WO&NR=2022144003A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHU, Jun</creatorcontrib><creatorcontrib>WAKABAYASHI, Yoshi</creatorcontrib><creatorcontrib>YANG, Chuxuan</creatorcontrib><creatorcontrib>BAI, Bing</creatorcontrib><creatorcontrib>ZHANG, Yongshu</creatorcontrib><title>METHOD FOR CONSTRUCTING MULTIPLEX PCR LIBRARY FOR HIGH-THROUGHPUT TARGETED SEQUENCING</title><description>A method for constructing a multiplex PCR library for high-throughput targeted sequencing: first acquiring a targeted DNA product by means of a high-specificity multiplex PCR reaction, and then digesting with a specific endonuclease, such that a specific molecular barcode is produced at the tail end of the PCR product; thus, the library construction process is more efficient, and the accuracy and sequencing depth of the obtained data are also ensured.
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| language | chi ; eng ; fre |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD FOR CONSTRUCTING MULTIPLEX PCR LIBRARY FOR HIGH-THROUGHPUT TARGETED SEQUENCING |
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