COMPOSITION FOR PREVENTING OR TREATING BONE DISEASES, INCLUDING CCR2 AS ACTIVE INGREDIENT

The present invention relates to a composition which is for preventing or treating bone diseases and includes CCR2 as an active ingredient. It was confirmed that a C-C chemokine receptor type 2 (CCR2) protein, a polynucleotide encoding the CCR2 protein, or a mesenchymal stem cell transduced with the CCR2 according to the present invention neutralize bone disease factor MCP-1, thereby reducing the collagen epitope (CTX-II) and collagen metabolism factors (MMP1 and MMP3) relating to collagen absorption in the body. In addition, it was confirmed that the expression of the SOX9 gene and anti-inflammatory cytokines (TGF-β and IL-10) related to cartilage differentiation was significantly increased. Thus, the present invention has excellent regenerative ability against osteoarthritis and excellent pain suppression and alleviation effects, and thus can be effectively used for preventing or treating bone diseases such as osteoarthritis. La présente invention concerne une composition pour la prévention ou le traitement de maladie des os et comprend CCR2 en tant que principe actif. Il a été confirmé qu'un récepteur de chimiokine C-C de type 2 (CCR2), un polynucléotide codant pour la protéine CCR2, ou une cellule souche mésenchymateuse transduite avec le CCR2 selon la présente invention, neutralisent le facteur MCP-1 de maladie des os, réduisant ainsi l'épitope de collagène (CTX-II) et des facteurs du métabolisme du collagène (MMP1 et MMP3) se rapportant à l'absorption du collagène dans le corps. De plus, il a été confirmé que l'expression du gène SOX9 et des cytokines anti-inflammatoires (TGF-β et IL-10) liées à la différenciation du cartilage était significativement augmentée. Ainsi, la présente invention présente une excellente capacité de régénération contre l'arthrose et d'excellents effets d'inhibition et de soulagement de la douleur, et peut donc être utilisée efficacement pour prévenir ou traiter des maladies des os telles que l'arthrose. 본 발명은 CCR2를 유효성분으로 포함하는 골질환의 예방 또는 치료용 조성물에 관한 것이다. 본 발명에 따른 CCR2(C-C chemokine receptor type 2) 단백질, CCR2 단백질을 암호화하는 폴리뉴클레오타이드 또는 CCR2가 형질도입된 중간엽줄기세포는 골 질환 인자인 MCP-1을 중화하여, 콜라겐 체내 흡수와 관련된 콜라겐 에피토프(CTX-Ⅱ) 및 콜라겐 대사인자(MMP1 및 MMP3)을 감소시킴을 확인하였다. 또한, 연골 분화와 관련된 SOX9 유전자, 항염증 사이토카인(TGF-β 및 IL-10)의 발현을 유의적으로 증가시킴을 확인하였다. 따라서, 골관절염에 대한 재생능이 우수하고 통증억제 및 완화 효과가 우수한 바, 골관절염과 같은 골질환의 예방 또는 치료에 유용하게 사용될 수 있다.