METHODS AND SYSTEMS FOR CELL LABELING AND IMAGING
The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element ma...
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| creator | ALEXEEV, Alexander CALERO-GARCIA, Miguel SULCHEK, Todd ZAMARAYEVA, Alla SICHER, Ian HAN, Sewoon |
| description | The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.
La présente invention concerne des procédés et des systèmes de traitement de cellules, comprenant l'administration d'agents d'imagerie dans des cellules. Les procédés et les systèmes peuvent comprendre l'utilisation d'un dispositif microfluidique. Le dispositif microfluidique peut comprendre un canal comprenant un élément de compression. L'élément de compression peut être configuré pour réduire un volume de la cellule et faciliter la formation d'un ou plusieurs pores transitoires dans une membrane cellulaire de la cellule. Le ou les pores peuvent permettre à un ou plusieurs agents d'imagerie d'entrer dans la cellule. L'invention concerne également des cellules modifiées produites à l'aide des procédés et systèmes de l'invention et des procédés d'imagerie des cellules modifiées chez un sujet. |
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La présente invention concerne des procédés et des systèmes de traitement de cellules, comprenant l'administration d'agents d'imagerie dans des cellules. Les procédés et les systèmes peuvent comprendre l'utilisation d'un dispositif microfluidique. Le dispositif microfluidique peut comprendre un canal comprenant un élément de compression. L'élément de compression peut être configuré pour réduire un volume de la cellule et faciliter la formation d'un ou plusieurs pores transitoires dans une membrane cellulaire de la cellule. Le ou les pores peuvent permettre à un ou plusieurs agents d'imagerie d'entrer dans la cellule. L'invention concerne également des cellules modifiées produites à l'aide des procédés et systèmes de l'invention et des procédés d'imagerie des cellules modifiées chez un sujet.</description><language>eng ; fre</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICALDEVICES ; MICROSTRUCTURAL TECHNOLOGY ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; SPIRITS ; TESTING ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201217&DB=EPODOC&CC=WO&NR=2020252215A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201217&DB=EPODOC&CC=WO&NR=2020252215A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ALEXEEV, Alexander</creatorcontrib><creatorcontrib>CALERO-GARCIA, Miguel</creatorcontrib><creatorcontrib>SULCHEK, Todd</creatorcontrib><creatorcontrib>ZAMARAYEVA, Alla</creatorcontrib><creatorcontrib>SICHER, Ian</creatorcontrib><creatorcontrib>HAN, Sewoon</creatorcontrib><title>METHODS AND SYSTEMS FOR CELL LABELING AND IMAGING</title><description>The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.
La présente invention concerne des procédés et des systèmes de traitement de cellules, comprenant l'administration d'agents d'imagerie dans des cellules. Les procédés et les systèmes peuvent comprendre l'utilisation d'un dispositif microfluidique. Le dispositif microfluidique peut comprendre un canal comprenant un élément de compression. L'élément de compression peut être configuré pour réduire un volume de la cellule et faciliter la formation d'un ou plusieurs pores transitoires dans une membrane cellulaire de la cellule. Le ou les pores peuvent permettre à un ou plusieurs agents d'imagerie d'entrer dans la cellule. 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The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.
La présente invention concerne des procédés et des systèmes de traitement de cellules, comprenant l'administration d'agents d'imagerie dans des cellules. Les procédés et les systèmes peuvent comprendre l'utilisation d'un dispositif microfluidique. Le dispositif microfluidique peut comprendre un canal comprenant un élément de compression. L'élément de compression peut être configuré pour réduire un volume de la cellule et faciliter la formation d'un ou plusieurs pores transitoires dans une membrane cellulaire de la cellule. Le ou les pores peuvent permettre à un ou plusieurs agents d'imagerie d'entrer dans la cellule. L'invention concerne également des cellules modifiées produites à l'aide des procédés et systèmes de l'invention et des procédés d'imagerie des cellules modifiées chez un sujet.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICALDEVICES MICROSTRUCTURAL TECHNOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPIRITS TESTING TRANSPORTING VINEGAR WINE |
| title | METHODS AND SYSTEMS FOR CELL LABELING AND IMAGING |
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