METHOD FOR THE GENERATION OF A NON-CRISPR BACTERIOPHAGE INSENSITIVE MUTANT USING AN ANTI-CRISPR PROTEIN
The invention provides a method for the generation of a bacteriophage insensitive mutant from parent strain cells comprising an active CRISPR-Cas system, the method comprising the stages: -an anti-CRISPR protein exposure stage comprising exposing the parent strain cells to an anti-CRISPR protein sui...
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| creator | BROUNS, Stan Johan Jozef DE NÓBREGA, Franklin Luzia |
| description | The invention provides a method for the generation of a bacteriophage insensitive mutant from parent strain cells comprising an active CRISPR-Cas system, the method comprising the stages: -an anti-CRISPR protein exposure stage comprising exposing the parent strain cells to an anti-CRISPR protein suitable for inhibiting the CRISPR-Cas system, wherein the anti-CRISPR protein comprises a protein comprising an amino acid sequence, wherein the amino acid sequence has at least 30% sequence identity with the amino acid sequence of SEQ ID NO:1with respect to a sequence alignment between the amino acid sequence and SEQ ID NO:1, and wherein the sequence alignment has a length ≥ 50% of the sequence length of SEQ ID NO:1; -a bacteriophage exposure stage comprising exposing the parent strain cells to a bacteriophage; -a selection stage comprising selecting the CRISPR-independent bacteriophage insensitive mutant from cells having survived the bacteriophage exposure stage.
Procédé de production d'un mutant insensible aux bactériophages à partir de cellules souches parentes comprenant un système CRISPR-Cas actif, le procédé comprenant les étapes suivantes : -exposition aux protéines anti-CRISPR, comprenant l'exposition des cellules souches parentes à une protéine anti-CRISPR appropriée pour Inhiber le système CRISPR-Cas, la protéine anti-CRISPR comprenant une protéine comportant une séquence d'acides aminés, la séquence d'acides aminés possédant au moins 30 % d'identité de séquence avec la séquence d'acides aminés de la SEQ ID NO: 1 par rapport à un alignement de séquence entre la séquence d'acides aminés et la SEQ ID NO : 1, et l'alignement de séquence ayant une longueur ≥ 50 % de la longueur de séquence de SEQ ID NO : 1; -exposition aux bactériophages, consistant à exposer les cellules souches parentes à un bactériophage; -sélection, consistant à sélectionner le mutant insensible aux bactériophages indépendant du CRISPR à partir de cellules ayant survécu l'étape d'exposition aux bactériophages. |
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Procédé de production d'un mutant insensible aux bactériophages à partir de cellules souches parentes comprenant un système CRISPR-Cas actif, le procédé comprenant les étapes suivantes : -exposition aux protéines anti-CRISPR, comprenant l'exposition des cellules souches parentes à une protéine anti-CRISPR appropriée pour Inhiber le système CRISPR-Cas, la protéine anti-CRISPR comprenant une protéine comportant une séquence d'acides aminés, la séquence d'acides aminés possédant au moins 30 % d'identité de séquence avec la séquence d'acides aminés de la SEQ ID NO: 1 par rapport à un alignement de séquence entre la séquence d'acides aminés et la SEQ ID NO : 1, et l'alignement de séquence ayant une longueur ≥ 50 % de la longueur de séquence de SEQ ID NO : 1; -exposition aux bactériophages, consistant à exposer les cellules souches parentes à un bactériophage; -sélection, consistant à sélectionner le mutant insensible aux bactériophages indépendant du CRISPR à partir de cellules ayant survécu l'étape d'exposition aux bactériophages.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE ; ENZYMOLOGY ; FOODS OR FOODSTUFFS ; HUMAN NECESSITIES ; MAKING THEREOF ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MILK OR CHEESE SUBSTITUTES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200522&DB=EPODOC&CC=WO&NR=2020101486A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200522&DB=EPODOC&CC=WO&NR=2020101486A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BROUNS, Stan Johan Jozef</creatorcontrib><creatorcontrib>DE NÓBREGA, Franklin Luzia</creatorcontrib><title>METHOD FOR THE GENERATION OF A NON-CRISPR BACTERIOPHAGE INSENSITIVE MUTANT USING AN ANTI-CRISPR PROTEIN</title><description>The invention provides a method for the generation of a bacteriophage insensitive mutant from parent strain cells comprising an active CRISPR-Cas system, the method comprising the stages: -an anti-CRISPR protein exposure stage comprising exposing the parent strain cells to an anti-CRISPR protein suitable for inhibiting the CRISPR-Cas system, wherein the anti-CRISPR protein comprises a protein comprising an amino acid sequence, wherein the amino acid sequence has at least 30% sequence identity with the amino acid sequence of SEQ ID NO:1with respect to a sequence alignment between the amino acid sequence and SEQ ID NO:1, and wherein the sequence alignment has a length ≥ 50% of the sequence length of SEQ ID NO:1; -a bacteriophage exposure stage comprising exposing the parent strain cells to a bacteriophage; -a selection stage comprising selecting the CRISPR-independent bacteriophage insensitive mutant from cells having survived the bacteriophage exposure stage.
Procédé de production d'un mutant insensible aux bactériophages à partir de cellules souches parentes comprenant un système CRISPR-Cas actif, le procédé comprenant les étapes suivantes : -exposition aux protéines anti-CRISPR, comprenant l'exposition des cellules souches parentes à une protéine anti-CRISPR appropriée pour Inhiber le système CRISPR-Cas, la protéine anti-CRISPR comprenant une protéine comportant une séquence d'acides aminés, la séquence d'acides aminés possédant au moins 30 % d'identité de séquence avec la séquence d'acides aminés de la SEQ ID NO: 1 par rapport à un alignement de séquence entre la séquence d'acides aminés et la SEQ ID NO : 1, et l'alignement de séquence ayant une longueur ≥ 50 % de la longueur de séquence de SEQ ID NO : 1; -exposition aux bactériophages, consistant à exposer les cellules souches parentes à un bactériophage; -sélection, consistant à sélectionner le mutant insensible aux bactériophages indépendant du CRISPR à partir de cellules ayant survécu l'étape d'exposition aux bactériophages.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE</subject><subject>ENZYMOLOGY</subject><subject>FOODS OR FOODSTUFFS</subject><subject>HUMAN NECESSITIES</subject><subject>MAKING THEREOF</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MILK OR CHEESE SUBSTITUTES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi7EKwkAMQLs4iPoPAedCW0VcY017GZqUu1THUuR0ES3U_0cH3YUHb3lvntwaMqdHqNSDOYKahDwaq4BWgCAqaek5tB4OWBp51tZhTcASSAIbnwiazlAMusBSA8oH49_VejViWSaz63Cf4urrRbKuyEqXxvHZx2kcLvERX_1Zi6zI8izf7neYb_6r3rXQNj4</recordid><startdate>20200522</startdate><enddate>20200522</enddate><creator>BROUNS, Stan Johan Jozef</creator><creator>DE NÓBREGA, Franklin Luzia</creator><scope>EVB</scope></search><sort><creationdate>20200522</creationdate><title>METHOD FOR THE GENERATION OF A NON-CRISPR BACTERIOPHAGE INSENSITIVE MUTANT USING AN ANTI-CRISPR PROTEIN</title><author>BROUNS, Stan Johan Jozef ; DE NÓBREGA, Franklin Luzia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2020101486A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE</topic><topic>ENZYMOLOGY</topic><topic>FOODS OR FOODSTUFFS</topic><topic>HUMAN NECESSITIES</topic><topic>MAKING THEREOF</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MILK OR CHEESE SUBSTITUTES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BROUNS, Stan Johan Jozef</creatorcontrib><creatorcontrib>DE NÓBREGA, Franklin Luzia</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BROUNS, Stan Johan Jozef</au><au>DE NÓBREGA, Franklin Luzia</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR THE GENERATION OF A NON-CRISPR BACTERIOPHAGE INSENSITIVE MUTANT USING AN ANTI-CRISPR PROTEIN</title><date>2020-05-22</date><risdate>2020</risdate><abstract>The invention provides a method for the generation of a bacteriophage insensitive mutant from parent strain cells comprising an active CRISPR-Cas system, the method comprising the stages: -an anti-CRISPR protein exposure stage comprising exposing the parent strain cells to an anti-CRISPR protein suitable for inhibiting the CRISPR-Cas system, wherein the anti-CRISPR protein comprises a protein comprising an amino acid sequence, wherein the amino acid sequence has at least 30% sequence identity with the amino acid sequence of SEQ ID NO:1with respect to a sequence alignment between the amino acid sequence and SEQ ID NO:1, and wherein the sequence alignment has a length ≥ 50% of the sequence length of SEQ ID NO:1; -a bacteriophage exposure stage comprising exposing the parent strain cells to a bacteriophage; -a selection stage comprising selecting the CRISPR-independent bacteriophage insensitive mutant from cells having survived the bacteriophage exposure stage.
Procédé de production d'un mutant insensible aux bactériophages à partir de cellules souches parentes comprenant un système CRISPR-Cas actif, le procédé comprenant les étapes suivantes : -exposition aux protéines anti-CRISPR, comprenant l'exposition des cellules souches parentes à une protéine anti-CRISPR appropriée pour Inhiber le système CRISPR-Cas, la protéine anti-CRISPR comprenant une protéine comportant une séquence d'acides aminés, la séquence d'acides aminés possédant au moins 30 % d'identité de séquence avec la séquence d'acides aminés de la SEQ ID NO: 1 par rapport à un alignement de séquence entre la séquence d'acides aminés et la SEQ ID NO : 1, et l'alignement de séquence ayant une longueur ≥ 50 % de la longueur de séquence de SEQ ID NO : 1; -exposition aux bactériophages, consistant à exposer les cellules souches parentes à un bactériophage; -sélection, consistant à sélectionner le mutant insensible aux bactériophages indépendant du CRISPR à partir de cellules ayant survécu l'étape d'exposition aux bactériophages.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE ENZYMOLOGY FOODS OR FOODSTUFFS HUMAN NECESSITIES MAKING THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILK OR CHEESE SUBSTITUTES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | METHOD FOR THE GENERATION OF A NON-CRISPR BACTERIOPHAGE INSENSITIVE MUTANT USING AN ANTI-CRISPR PROTEIN |
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