CULTURE MEDIUM FOR TISSUE CULTURE OF FAGUS LONGIPETIOLATA PLANTS AND CULTURE METHOD THEREFOR

A culture medium for tissue culture of fagus longipetiolata plants. A material drawing period in a year is April; a culture medium for starting culture is as follows: leaf, MS + 2,4-D 2.0 mg/L + 6-BA 1.0 mg/L + saccharose 25 g/L, and stem, MS + 2,4-D 1.0 mg/L + 6-BA 1.5 mg/L + saccharose 30 g/L; a s...

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Hauptverfasser: MA, Yueqin, HU, Jinyao, BIE, Pengfei, WANG, Hong, JIANG, Wei, XIANG, Li
Format: Patent
Sprache:chi ; eng ; fre
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Zusammenfassung:A culture medium for tissue culture of fagus longipetiolata plants. A material drawing period in a year is April; a culture medium for starting culture is as follows: leaf, MS + 2,4-D 2.0 mg/L + 6-BA 1.0 mg/L + saccharose 25 g/L, and stem, MS + 2,4-D 1.0 mg/L + 6-BA 1.5 mg/L + saccharose 30 g/L; a sugar source having the effect of promoting the growth of callus is MS + 6-BA 3.0 mg/L + NAA 0.5 mg/L + saccharose 20 g/L; and a culture medium for rooting is 1/2 MS + NAA 1.5 mg/L + saccharose 30 g/L. A method for culturing fagus longipetiolata plants by using the culture medium and fagus longipetiolata plants cultured using the culture medium. The method can select a suitable medium in a short time and has high reliability. L'invention concerne un milieu de culture pour la culture tissulaire de plantes fagus longipetiolata. Une période d'extraction du matériau dans l'année est avril ; un milieu de culture pour le démarrage d'une culture est le suivant : feuille, MS + 2,4-D 2,0 mg/L + 6-BA 1,0 mg/L + saccharose 25