SECOND-GENERATION HIGH-THROUGHPUT SEQUENCING LIBRARY CONSTRUCTION METHOD

Provided are a second-generation high-throughput sequencing library construction method, and a kit. The present invention improves the utilization rate of a nucleic acid template, simplifies the construction procedure of a sequencing library and makes a sequencing result more accurate and the degree...

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Hauptverfasser: CHEN, Qiong, CHANG, Luyuan, GUO, Huanhuan, CHEN, Wenhao, WANG, Xiaoqing, JING, Zhongying, QUAN, Shengmao, LIANG, Lili, ZHANG, Yi
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creator CHEN, Qiong
CHANG, Luyuan
GUO, Huanhuan
CHEN, Wenhao
WANG, Xiaoqing
JING, Zhongying
QUAN, Shengmao
LIANG, Lili
ZHANG, Yi
description Provided are a second-generation high-throughput sequencing library construction method, and a kit. The present invention improves the utilization rate of a nucleic acid template, simplifies the construction procedure of a sequencing library and makes a sequencing result more accurate and the degree of coverage more uniform. L'invention concerne un procédé de construction de bibliothèque de séquençage à haut rendement de seconde génération, et un kit. La présente invention améliore le taux d'utilisation d'un modèle d'acide nucléique, simplifie la procédure de construction d'une bibliothèque de séquençage et rend un résultat de séquençage plus précis et le degré de couverture plus uniforme. 提供了一种用于第二代高通量测序文库构建的方法和试剂盒。提高了核酸模板的利用效率,简化了测序文库的构建流程,使得测序结果更加准确和覆盖度更加均一。
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMBINATORIAL CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title SECOND-GENERATION HIGH-THROUGHPUT SEQUENCING LIBRARY CONSTRUCTION METHOD
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