METHOD OF DETERMINATION OF HYALURONIC ACID
The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycan...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | VELEBNY, Vladimir HRUDIKOVA, Radka JILKOVA, Jana PAVLIK, Jaroslav PEPELIAEV, Stanislav |
| description | The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycans, these can be separated by precipitation before the cleavage, e.g. with the use of cetyltrimethylammomumbromide (CTAB) in acetate buffer and salt medium. In case of a high content of reducing sugars, e.g. glucose, their influence can be eliminated, after removing the glycosaminoglycans and before the cleavage, by precipitating hyaluronic acid from the formed supernatant and then redissolving this sediment in acetate buffer and performing the cleavage step.
L'invention concerne un procédé de détermination de la présence d'acide hyaluronique dans des échantillons complexes par clivage à l'aide de l'enzyme SpnHyl, puis réaction avec de la 3-méthyl-2-benzothiazolinone hydrazone (MBTH) pour former un produit coloré analysé par spectrophotométrie. Dans le cas d'une teneur élevée en glycosaminoglycanes, ceux-ci peuvent être séparés par précipitation avant le clivage, par exemple en utilisant du bromure de cétyltriméthylammonium (CTAB) dans un tampon acétate et un milieu salin. Dans le cas d'une teneur élevée en sucres réducteurs, par exemple le glucose, leur influence peut être éliminée, après élimination des glycosaminoglycanes et avant le clivage, par précipitation de l'acide hyaluronique hors du surnageant formé, puis re-dissolution de ce sédiment dans du tampon acétate et mise en œuvre de l'étape de clivage. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2018166545A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2018166545A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2018166545A13</originalsourceid><addsrcrecordid>eNrjZNDydQ3x8HdR8HdTcHENcQ3y9fRzDPH09wMJeEQ6-oQG-ft5Ois4Onu68DCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-HB_IwNDC0MzM1MTU0dDY-JUAQDWPiV6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHOD OF DETERMINATION OF HYALURONIC ACID</title><source>esp@cenet</source><creator>VELEBNY, Vladimir ; HRUDIKOVA, Radka ; JILKOVA, Jana ; PAVLIK, Jaroslav ; PEPELIAEV, Stanislav</creator><creatorcontrib>VELEBNY, Vladimir ; HRUDIKOVA, Radka ; JILKOVA, Jana ; PAVLIK, Jaroslav ; PEPELIAEV, Stanislav</creatorcontrib><description>The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycans, these can be separated by precipitation before the cleavage, e.g. with the use of cetyltrimethylammomumbromide (CTAB) in acetate buffer and salt medium. In case of a high content of reducing sugars, e.g. glucose, their influence can be eliminated, after removing the glycosaminoglycans and before the cleavage, by precipitating hyaluronic acid from the formed supernatant and then redissolving this sediment in acetate buffer and performing the cleavage step.
L'invention concerne un procédé de détermination de la présence d'acide hyaluronique dans des échantillons complexes par clivage à l'aide de l'enzyme SpnHyl, puis réaction avec de la 3-méthyl-2-benzothiazolinone hydrazone (MBTH) pour former un produit coloré analysé par spectrophotométrie. Dans le cas d'une teneur élevée en glycosaminoglycanes, ceux-ci peuvent être séparés par précipitation avant le clivage, par exemple en utilisant du bromure de cétyltriméthylammonium (CTAB) dans un tampon acétate et un milieu salin. Dans le cas d'une teneur élevée en sucres réducteurs, par exemple le glucose, leur influence peut être éliminée, après élimination des glycosaminoglycanes et avant le clivage, par précipitation de l'acide hyaluronique hors du surnageant formé, puis re-dissolution de ce sédiment dans du tampon acétate et mise en œuvre de l'étape de clivage.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS BASED THEREON ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; DERIVATIVES THEREOF ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC MACROMOLECULAR COMPOUNDS ; POLYSACCHARIDES ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; THEIR PREPARATION OR CHEMICAL WORKING-UP ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180920&DB=EPODOC&CC=WO&NR=2018166545A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180920&DB=EPODOC&CC=WO&NR=2018166545A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VELEBNY, Vladimir</creatorcontrib><creatorcontrib>HRUDIKOVA, Radka</creatorcontrib><creatorcontrib>JILKOVA, Jana</creatorcontrib><creatorcontrib>PAVLIK, Jaroslav</creatorcontrib><creatorcontrib>PEPELIAEV, Stanislav</creatorcontrib><title>METHOD OF DETERMINATION OF HYALURONIC ACID</title><description>The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycans, these can be separated by precipitation before the cleavage, e.g. with the use of cetyltrimethylammomumbromide (CTAB) in acetate buffer and salt medium. In case of a high content of reducing sugars, e.g. glucose, their influence can be eliminated, after removing the glycosaminoglycans and before the cleavage, by precipitating hyaluronic acid from the formed supernatant and then redissolving this sediment in acetate buffer and performing the cleavage step.
L'invention concerne un procédé de détermination de la présence d'acide hyaluronique dans des échantillons complexes par clivage à l'aide de l'enzyme SpnHyl, puis réaction avec de la 3-méthyl-2-benzothiazolinone hydrazone (MBTH) pour former un produit coloré analysé par spectrophotométrie. Dans le cas d'une teneur élevée en glycosaminoglycanes, ceux-ci peuvent être séparés par précipitation avant le clivage, par exemple en utilisant du bromure de cétyltriméthylammonium (CTAB) dans un tampon acétate et un milieu salin. Dans le cas d'une teneur élevée en sucres réducteurs, par exemple le glucose, leur influence peut être éliminée, après élimination des glycosaminoglycanes et avant le clivage, par précipitation de l'acide hyaluronique hors du surnageant formé, puis re-dissolution de ce sédiment dans du tampon acétate et mise en œuvre de l'étape de clivage.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS BASED THEREON</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC MACROMOLECULAR COMPOUNDS</subject><subject>POLYSACCHARIDES</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNDydQ3x8HdR8HdTcHENcQ3y9fRzDPH09wMJeEQ6-oQG-ft5Ois4Onu68DCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-HB_IwNDC0MzM1MTU0dDY-JUAQDWPiV6</recordid><startdate>20180920</startdate><enddate>20180920</enddate><creator>VELEBNY, Vladimir</creator><creator>HRUDIKOVA, Radka</creator><creator>JILKOVA, Jana</creator><creator>PAVLIK, Jaroslav</creator><creator>PEPELIAEV, Stanislav</creator><scope>EVB</scope></search><sort><creationdate>20180920</creationdate><title>METHOD OF DETERMINATION OF HYALURONIC ACID</title><author>VELEBNY, Vladimir ; HRUDIKOVA, Radka ; JILKOVA, Jana ; PAVLIK, Jaroslav ; PEPELIAEV, Stanislav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2018166545A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS BASED THEREON</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC MACROMOLECULAR COMPOUNDS</topic><topic>POLYSACCHARIDES</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR CHEMICAL WORKING-UP</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>VELEBNY, Vladimir</creatorcontrib><creatorcontrib>HRUDIKOVA, Radka</creatorcontrib><creatorcontrib>JILKOVA, Jana</creatorcontrib><creatorcontrib>PAVLIK, Jaroslav</creatorcontrib><creatorcontrib>PEPELIAEV, Stanislav</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VELEBNY, Vladimir</au><au>HRUDIKOVA, Radka</au><au>JILKOVA, Jana</au><au>PAVLIK, Jaroslav</au><au>PEPELIAEV, Stanislav</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF DETERMINATION OF HYALURONIC ACID</title><date>2018-09-20</date><risdate>2018</risdate><abstract>The invention relates to a method of determination of hyaluronic acid in complex samples by cleavage with SpnHyl enzyme followed by the reaction with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a coloured product analysed by spectrophotometry. In case of a high content of glycosaminoglycans, these can be separated by precipitation before the cleavage, e.g. with the use of cetyltrimethylammomumbromide (CTAB) in acetate buffer and salt medium. In case of a high content of reducing sugars, e.g. glucose, their influence can be eliminated, after removing the glycosaminoglycans and before the cleavage, by precipitating hyaluronic acid from the formed supernatant and then redissolving this sediment in acetate buffer and performing the cleavage step.
L'invention concerne un procédé de détermination de la présence d'acide hyaluronique dans des échantillons complexes par clivage à l'aide de l'enzyme SpnHyl, puis réaction avec de la 3-méthyl-2-benzothiazolinone hydrazone (MBTH) pour former un produit coloré analysé par spectrophotométrie. Dans le cas d'une teneur élevée en glycosaminoglycanes, ceux-ci peuvent être séparés par précipitation avant le clivage, par exemple en utilisant du bromure de cétyltriméthylammonium (CTAB) dans un tampon acétate et un milieu salin. Dans le cas d'une teneur élevée en sucres réducteurs, par exemple le glucose, leur influence peut être éliminée, après élimination des glycosaminoglycanes et avant le clivage, par précipitation de l'acide hyaluronique hors du surnageant formé, puis re-dissolution de ce sédiment dans du tampon acétate et mise en œuvre de l'étape de clivage.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO2018166545A1 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS POLYSACCHARIDES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE |
| title | METHOD OF DETERMINATION OF HYALURONIC ACID |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T01%3A51%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=VELEBNY,%20Vladimir&rft.date=2018-09-20&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO2018166545A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |