CELL CULTURE MEDIUM AND CULTURE METHOD FOR INCREASING PURITY OF ANTIBODY

Provided is a homemade supplemented medium that contains 90 mM to 500 mM of cysteine. Also provided is a cell culture method, comprising the steps of: inoculating a mammalian cell comprising a nucleic acid encoding an antibody into a basal medium; adding the homemade supplemented medium after enteri...

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Bibliographische Detailangaben
Hauptverfasser:	SUN, Wenzheng, CHENG, Xiwen, YAN, Chengwei, WENG, Yuancan, YANG, Bin, DENG, Chongfei
Format:	Patent
Sprache:	chi ; eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a homemade supplemented medium that contains 90 mM to 500 mM of cysteine. Also provided is a cell culture method, comprising the steps of: inoculating a mammalian cell comprising a nucleic acid encoding an antibody into a basal medium; adding the homemade supplemented medium after entering the cellular proliferative stage; and monitoring the cell density to adjust the culture time. L'invention concerne un milieu maison complété qui contient 90 mM à 500 mM de cystéine. L'invention porte également sur un procédé de culture cellulaire, comprenant les étapes consistant à : inoculer une cellule mammifère comprenant un acide nucléique codant pour un anticorps dans un milieu basal ; ajouter le milieu maison complété après l'entrée dans l'étage de prolifération cellulaire ; et surveiller la densité cellulaire pour ajuster le temps de culture. 提供了一种自制补料培养基，所述培养基含有90mM～500mM半胱氨酸。并提供了一种细胞培养方法，包括以下步骤：在基础培养基中接种包含编码抗体核酸的哺乳动物细胞；进入细胞增殖期后补加所述自制补料培养基；监测细胞密度调整培养时间。