MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY
The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for...
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| creator | SCHWARZ, Wolfgang LEIS, Benedikt LIEBL, Wolfgang PRECHTL, Roman ZVERLOV, Vladimir HELD, Claudia |
| description | The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for producing said mutant variants of the β-glucosidase Cgl T. Also provided are artificial cellulosomes comprising the mutant variants of the β-glucosidase Cgl T and methods for the enzymatic hydrolysis of cellulosic biomass comprising said artificial cellulosomes and/or said mutant variants of the β-glucosidase Cgl T.
La présente invention concerne des variants mutants de la β-glucosidase Cgl T provenant de Thermoanaerobacter brockii et des acides nucléiques pour les produire. Lesdits variants mutants présentent une thermostabilité et une activité enzymatique significativement accrues. En outre, l'invention décrit des vecteurs, des cellules hôtes et des procédés de production desdits variants mutants de la β-glucosidase Cgl T. Sont également décrits des cellulosomes artificiels comprenant des variants mutants de la β-glucosidase Cgl T et des procédés d'hydrolyse enzymatique de biomasse cellulosique comprenant lesdits cellulosomes artificiels et/ou variants mutants de la β-glucosidase Cgl T. |
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La présente invention concerne des variants mutants de la β-glucosidase Cgl T provenant de Thermoanaerobacter brockii et des acides nucléiques pour les produire. Lesdits variants mutants présentent une thermostabilité et une activité enzymatique significativement accrues. En outre, l'invention décrit des vecteurs, des cellules hôtes et des procédés de production desdits variants mutants de la β-glucosidase Cgl T. Sont également décrits des cellulosomes artificiels comprenant des variants mutants de la β-glucosidase Cgl T et des procédés d'hydrolyse enzymatique de biomasse cellulosique comprenant lesdits cellulosomes artificiels et/ou variants mutants de la β-glucosidase Cgl T.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170420&DB=EPODOC&CC=WO&NR=2017063787A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170420&DB=EPODOC&CC=WO&NR=2017063787A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SCHWARZ, Wolfgang</creatorcontrib><creatorcontrib>LEIS, Benedikt</creatorcontrib><creatorcontrib>LIEBL, Wolfgang</creatorcontrib><creatorcontrib>PRECHTL, Roman</creatorcontrib><creatorcontrib>ZVERLOV, Vladimir</creatorcontrib><creatorcontrib>HELD, Claudia</creatorcontrib><title>MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY</title><description>The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for producing said mutant variants of the β-glucosidase Cgl T. Also provided are artificial cellulosomes comprising the mutant variants of the β-glucosidase Cgl T and methods for the enzymatic hydrolysis of cellulosic biomass comprising said artificial cellulosomes and/or said mutant variants of the β-glucosidase Cgl T.
La présente invention concerne des variants mutants de la β-glucosidase Cgl T provenant de Thermoanaerobacter brockii et des acides nucléiques pour les produire. Lesdits variants mutants présentent une thermostabilité et une activité enzymatique significativement accrues. En outre, l'invention décrit des vecteurs, des cellules hôtes et des procédés de production desdits variants mutants de la β-glucosidase Cgl T. Sont également décrits des cellulosomes artificiels comprenant des variants mutants de la β-glucosidase Cgl T et des procédés d'hydrolyse enzymatique de biomasse cellulosique comprenant lesdits cellulosomes artificiels et/ou variants mutants de la β-glucosidase Cgl T.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD3DQ1x9AtRcHINcdR19wl19g_2dHEMdlUIcwzyBEoEK4R7hngoePo5B7kChV0UQjxcg3z9g0McnTx9PEMieRhY0xJzilN5oTQ3g7Kba4izh25qQX58anFBYnJqXmpJfLi_kYGhuYGZsbmFuaOhMXGqAF_nK9M</recordid><startdate>20170420</startdate><enddate>20170420</enddate><creator>SCHWARZ, Wolfgang</creator><creator>LEIS, Benedikt</creator><creator>LIEBL, Wolfgang</creator><creator>PRECHTL, Roman</creator><creator>ZVERLOV, Vladimir</creator><creator>HELD, Claudia</creator><scope>EVB</scope></search><sort><creationdate>20170420</creationdate><title>MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY</title><author>SCHWARZ, Wolfgang ; LEIS, Benedikt ; LIEBL, Wolfgang ; PRECHTL, Roman ; ZVERLOV, Vladimir ; HELD, Claudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2017063787A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SCHWARZ, Wolfgang</creatorcontrib><creatorcontrib>LEIS, Benedikt</creatorcontrib><creatorcontrib>LIEBL, Wolfgang</creatorcontrib><creatorcontrib>PRECHTL, Roman</creatorcontrib><creatorcontrib>ZVERLOV, Vladimir</creatorcontrib><creatorcontrib>HELD, Claudia</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SCHWARZ, Wolfgang</au><au>LEIS, Benedikt</au><au>LIEBL, Wolfgang</au><au>PRECHTL, Roman</au><au>ZVERLOV, Vladimir</au><au>HELD, Claudia</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY</title><date>2017-04-20</date><risdate>2017</risdate><abstract>The invention relates to mutant variants of the β-glucosidase Cgl T from Thermoanaerobacter brockii and nucleic acids for producing the same. Said mutant variants show significantly increased thermostability and enzyme activity. Furthermore, the invention provides vectors, host cells and methods for producing said mutant variants of the β-glucosidase Cgl T. Also provided are artificial cellulosomes comprising the mutant variants of the β-glucosidase Cgl T and methods for the enzymatic hydrolysis of cellulosic biomass comprising said artificial cellulosomes and/or said mutant variants of the β-glucosidase Cgl T.
La présente invention concerne des variants mutants de la β-glucosidase Cgl T provenant de Thermoanaerobacter brockii et des acides nucléiques pour les produire. Lesdits variants mutants présentent une thermostabilité et une activité enzymatique significativement accrues. En outre, l'invention décrit des vecteurs, des cellules hôtes et des procédés de production desdits variants mutants de la β-glucosidase Cgl T. Sont également décrits des cellulosomes artificiels comprenant des variants mutants de la β-glucosidase Cgl T et des procédés d'hydrolyse enzymatique de biomasse cellulosique comprenant lesdits cellulosomes artificiels et/ou variants mutants de la β-glucosidase Cgl T.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | MUTANT BETA-GLUCOSIDASE VARIANTS WITH INCREASED THERMOSTABILITY |
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