OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS

Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain,...

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Hauptverfasser: KLEIN, Theodore Mitchell, ROSEN, Barabara Ann Marie, BASS, Steven Henry, CHO, Hyeon-Je, WEI, Jun-Zhi, MOY, York, LASSNER, Michael, ANAND, Ajith, BERTAIN, Sean M, KINNEY, Anthony J, MCBRIDE, Kevin E
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creator KLEIN, Theodore Mitchell
ROSEN, Barabara Ann Marie
BASS, Steven Henry
CHO, Hyeon-Je
WEI, Jun-Zhi
MOY, York
LASSNER, Michael
ANAND, Ajith
BERTAIN, Sean M
KINNEY, Anthony J
MCBRIDE, Kevin E
description Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided. L'invention concerne des procédés et des compositions destinés à la transformation de plantes médiée par Ochrobactrum. Les procédés comprennent, mais sans s'y limiter, l'utilisation d'une souche d'Ochrobactrum pour transférer un polynucléotide d'intérêt à une cellule végétale. Ces derniers comprennent des procédés dépendant de VirD2. Les compositions contiennent une souche d'Ochrobactrum, des ADN, des constructions et/ou des plasmides de transfert. Ces éléments comprennent des souches d'Ochrobactrum ayant un plasmide comprenant un ou plusieurs gène(s) de virulence, une région de bordure et/ou une origine de réplication. Des cellules végétales, des tissus, des plantes et des graines comprenant un polynucléotide d'intérêt produits au moyen de ces procédés sont également décrits.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title OCHROBACTRUM-MEDIATED TRANSFORMATION OF PLANTS
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