METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES
Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolati...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | AL-ABDULLAH, Ismail KALKUM, Markus |
| description | Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2017027867A2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2017027867A2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2017027867A23</originalsourceid><addsrcrecordid>eNrjZHDxdQ3x8HcJ1lFw9vcN8A_2DPH09wPyHP1cFLw9Q4IV3PyDFFxcQ1ydQRIK_m4KTo7OIa5Bno4-CgFB_iGujsGuwTwMrGmJOcWpvFCam0HZzTXE2UM3tSA_PrW4IDE5NS-1JD7c38jA0NzAyNzCzNzRyJg4VQD6Niym</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES</title><source>esp@cenet</source><creator>AL-ABDULLAH, Ismail ; KALKUM, Markus</creator><creatorcontrib>AL-ABDULLAH, Ismail ; KALKUM, Markus</creatorcontrib><description>Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170216&DB=EPODOC&CC=WO&NR=2017027867A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170216&DB=EPODOC&CC=WO&NR=2017027867A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>AL-ABDULLAH, Ismail</creatorcontrib><creatorcontrib>KALKUM, Markus</creatorcontrib><title>METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES</title><description>Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDxdQ3x8HcJ1lFw9vcN8A_2DPH09wPyHP1cFLw9Q4IV3PyDFFxcQ1ydQRIK_m4KTo7OIa5Bno4-CgFB_iGujsGuwTwMrGmJOcWpvFCam0HZzTXE2UM3tSA_PrW4IDE5NS-1JD7c38jA0NzAyNzCzNzRyJg4VQD6Niym</recordid><startdate>20170216</startdate><enddate>20170216</enddate><creator>AL-ABDULLAH, Ismail</creator><creator>KALKUM, Markus</creator><scope>EVB</scope></search><sort><creationdate>20170216</creationdate><title>METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES</title><author>AL-ABDULLAH, Ismail ; KALKUM, Markus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2017027867A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>AL-ABDULLAH, Ismail</creatorcontrib><creatorcontrib>KALKUM, Markus</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>AL-ABDULLAH, Ismail</au><au>KALKUM, Markus</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES</title><date>2017-02-16</date><risdate>2017</risdate><abstract>Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO2017027867A2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF BACTERIAL PROTEASES |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T00%3A53%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=AL-ABDULLAH,%20Ismail&rft.date=2017-02-16&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO2017027867A2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |