METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS
The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA deple...
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| creator | SEREBROV, Victor JOLLY, Samson, Michael SALOMON, William, Edward ZHANG, Han MOORE, Melissa, Jeanne ZAMORE, Phillip, David |
| description | The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.
L'invention concerne l'utilisation de complexes de molécules-guides de polypeptides argonautes en tant que sondes rapides et spécifiques de l'acide nucléique, en tant qu'enzymes de restriction spécifiques guidés par l'acide nucléique pour des substrats ADN et ARN, et en tant que moyen permettant la détection des interactions des protéines ARN, la détection de l'ARN, la détection de l'ADN, et l'appauvrissement de l'ARN. L'utilisation desdits complexes de molécule- guides de polypeptides argonautes permet la détection rapide et spécifique, la purification, et l'activité enzymatique. |
| format | Patent |
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L'invention concerne l'utilisation de complexes de molécules-guides de polypeptides argonautes en tant que sondes rapides et spécifiques de l'acide nucléique, en tant qu'enzymes de restriction spécifiques guidés par l'acide nucléique pour des substrats ADN et ARN, et en tant que moyen permettant la détection des interactions des protéines ARN, la détection de l'ARN, la détection de l'ADN, et l'appauvrissement de l'ARN. L'utilisation desdits complexes de molécule- guides de polypeptides argonautes permet la détection rapide et spécifique, la purification, et l'activité enzymatique.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161110&DB=EPODOC&CC=WO&NR=2016161375A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161110&DB=EPODOC&CC=WO&NR=2016161375A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SEREBROV, Victor</creatorcontrib><creatorcontrib>JOLLY, Samson, Michael</creatorcontrib><creatorcontrib>SALOMON, William, Edward</creatorcontrib><creatorcontrib>ZHANG, Han</creatorcontrib><creatorcontrib>MOORE, Melissa, Jeanne</creatorcontrib><creatorcontrib>ZAMORE, Phillip, David</creatorcontrib><title>METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS</title><description>The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.
L'invention concerne l'utilisation de complexes de molécules-guides de polypeptides argonautes en tant que sondes rapides et spécifiques de l'acide nucléique, en tant qu'enzymes de restriction spécifiques guidés par l'acide nucléique pour des substrats ADN et ARN, et en tant que moyen permettant la détection des interactions des protéines ARN, la détection de l'ARN, la détection de l'ADN, et l'appauvrissement de l'ARN. L'utilisation desdits complexes de molécule- guides de polypeptides argonautes permet la détection rapide et spécifique, la purification, et l'activité enzymatique.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLDydQ3x8HcJVvB3UwgN9vRzV_D38XT39wt19nH1D_F0cdV1DwWSLgqOQUBRx9AQV4WAIP8QV0-_YB4G1rTEnOJUXijNzaDs5hri7KGbWpAfn1pckJicmpdaEh_ub2RgaAaExuamjsbGxKkCAHqUKkM</recordid><startdate>20161110</startdate><enddate>20161110</enddate><creator>SEREBROV, Victor</creator><creator>JOLLY, Samson, Michael</creator><creator>SALOMON, William, Edward</creator><creator>ZHANG, Han</creator><creator>MOORE, Melissa, Jeanne</creator><creator>ZAMORE, Phillip, David</creator><scope>EVB</scope></search><sort><creationdate>20161110</creationdate><title>METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS</title><author>SEREBROV, Victor ; JOLLY, Samson, Michael ; SALOMON, William, Edward ; ZHANG, Han ; MOORE, Melissa, Jeanne ; ZAMORE, Phillip, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2016161375A33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SEREBROV, Victor</creatorcontrib><creatorcontrib>JOLLY, Samson, Michael</creatorcontrib><creatorcontrib>SALOMON, William, Edward</creatorcontrib><creatorcontrib>ZHANG, Han</creatorcontrib><creatorcontrib>MOORE, Melissa, Jeanne</creatorcontrib><creatorcontrib>ZAMORE, Phillip, David</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SEREBROV, Victor</au><au>JOLLY, Samson, Michael</au><au>SALOMON, William, Edward</au><au>ZHANG, Han</au><au>MOORE, Melissa, Jeanne</au><au>ZAMORE, Phillip, David</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS</title><date>2016-11-10</date><risdate>2016</risdate><abstract>The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.
L'invention concerne l'utilisation de complexes de molécules-guides de polypeptides argonautes en tant que sondes rapides et spécifiques de l'acide nucléique, en tant qu'enzymes de restriction spécifiques guidés par l'acide nucléique pour des substrats ADN et ARN, et en tant que moyen permettant la détection des interactions des protéines ARN, la détection de l'ARN, la détection de l'ADN, et l'appauvrissement de l'ARN. L'utilisation desdits complexes de molécule- guides de polypeptides argonautes permet la détection rapide et spécifique, la purification, et l'activité enzymatique.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS |
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