METHOD FOR ISOLATING EXOSOMES
The invention relates to a method for isolating exosomes from a biological fluid, said method including at least the following two consecutive steps of affinity purification: a) a first step using at least one specific anti-ligand of a generic exosome ligand, in order to obtain a population P of exo...
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| creator | OTT, CATHERINE GENERENAZ, LAURENCE MALLET, FRANÇOIS |
| description | The invention relates to a method for isolating exosomes from a biological fluid, said method including at least the following two consecutive steps of affinity purification: a) a first step using at least one specific anti-ligand of a generic exosome ligand, in order to obtain a population P of exosomes, said exosomes being separated from said anti-ligand; and b) a second step, applied to the population P of exosomes, using at least one anti-ligand specific to a ligand characteristic of a sub-population SP of exosomes, in order to obtain said sub-population SP of exosomes, said exosomes optionally being separated from said anti-ligand. The invention also relates to the uses of such a method.
L'invention concerne un procédé d'isolement d'exosomes à partir d'un liquide biologique, comprenantau moins deux étapes successives de purification par affinité suivantes: a)une première étape utilisant au moins un anti-ligand spécifique d'un ligand générique des exosomes, pour obtenir une population P d'exosomes, lesdits exosomes étant séparés dudit anti-ligand, et b)une seconde étape, appliquée à la population P d'exosomes, utilisant au moins un anti-ligand spécifique d'un ligand caractéristique d'une sous-population SP d'exosomes, pour obtenir ladite sous-population SP d'exosomes, lesdits exosomes étantséparés dudit anti-ligand ou non.. ainsi que les applications d'un tel procédé. |
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L'invention concerne un procédé d'isolement d'exosomes à partir d'un liquide biologique, comprenantau moins deux étapes successives de purification par affinité suivantes: a)une première étape utilisant au moins un anti-ligand spécifique d'un ligand générique des exosomes, pour obtenir une population P d'exosomes, lesdits exosomes étant séparés dudit anti-ligand, et b)une seconde étape, appliquée à la population P d'exosomes, utilisant au moins un anti-ligand spécifique d'un ligand caractéristique d'une sous-population SP d'exosomes, pour obtenir ladite sous-population SP d'exosomes, lesdits exosomes étantséparés dudit anti-ligand ou non.. ainsi que les applications d'un tel procédé.</description><language>eng ; fre</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150611&DB=EPODOC&CC=WO&NR=2015082839A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20150611&DB=EPODOC&CC=WO&NR=2015082839A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OTT, CATHERINE</creatorcontrib><creatorcontrib>GENERENAZ, LAURENCE</creatorcontrib><creatorcontrib>MALLET, FRANÇOIS</creatorcontrib><title>METHOD FOR ISOLATING EXOSOMES</title><description>The invention relates to a method for isolating exosomes from a biological fluid, said method including at least the following two consecutive steps of affinity purification: a) a first step using at least one specific anti-ligand of a generic exosome ligand, in order to obtain a population P of exosomes, said exosomes being separated from said anti-ligand; and b) a second step, applied to the population P of exosomes, using at least one anti-ligand specific to a ligand characteristic of a sub-population SP of exosomes, in order to obtain said sub-population SP of exosomes, said exosomes optionally being separated from said anti-ligand. The invention also relates to the uses of such a method.
L'invention concerne un procédé d'isolement d'exosomes à partir d'un liquide biologique, comprenantau moins deux étapes successives de purification par affinité suivantes: a)une première étape utilisant au moins un anti-ligand spécifique d'un ligand générique des exosomes, pour obtenir une population P d'exosomes, lesdits exosomes étant séparés dudit anti-ligand, et b)une seconde étape, appliquée à la population P d'exosomes, utilisant au moins un anti-ligand spécifique d'un ligand caractéristique d'une sous-population SP d'exosomes, pour obtenir ladite sous-population SP d'exosomes, lesdits exosomes étantséparés dudit anti-ligand ou non.. ainsi que les applications d'un tel procédé.</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJD1dQ3x8HdRcPMPUvAM9vdxDPH0c1dwjfAP9vd1DeZhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGhqYGFkYWxpaOhsbEqQIAIPEiJQ</recordid><startdate>20150611</startdate><enddate>20150611</enddate><creator>OTT, CATHERINE</creator><creator>GENERENAZ, LAURENCE</creator><creator>MALLET, FRANÇOIS</creator><scope>EVB</scope></search><sort><creationdate>20150611</creationdate><title>METHOD FOR ISOLATING EXOSOMES</title><author>OTT, CATHERINE ; GENERENAZ, LAURENCE ; MALLET, FRANÇOIS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2015082839A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2015</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>OTT, CATHERINE</creatorcontrib><creatorcontrib>GENERENAZ, LAURENCE</creatorcontrib><creatorcontrib>MALLET, FRANÇOIS</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>OTT, CATHERINE</au><au>GENERENAZ, LAURENCE</au><au>MALLET, FRANÇOIS</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR ISOLATING EXOSOMES</title><date>2015-06-11</date><risdate>2015</risdate><abstract>The invention relates to a method for isolating exosomes from a biological fluid, said method including at least the following two consecutive steps of affinity purification: a) a first step using at least one specific anti-ligand of a generic exosome ligand, in order to obtain a population P of exosomes, said exosomes being separated from said anti-ligand; and b) a second step, applied to the population P of exosomes, using at least one anti-ligand specific to a ligand characteristic of a sub-population SP of exosomes, in order to obtain said sub-population SP of exosomes, said exosomes optionally being separated from said anti-ligand. The invention also relates to the uses of such a method.
L'invention concerne un procédé d'isolement d'exosomes à partir d'un liquide biologique, comprenantau moins deux étapes successives de purification par affinité suivantes: a)une première étape utilisant au moins un anti-ligand spécifique d'un ligand générique des exosomes, pour obtenir une population P d'exosomes, lesdits exosomes étant séparés dudit anti-ligand, et b)une seconde étape, appliquée à la population P d'exosomes, utilisant au moins un anti-ligand spécifique d'un ligand caractéristique d'une sous-population SP d'exosomes, pour obtenir ladite sous-population SP d'exosomes, lesdits exosomes étantséparés dudit anti-ligand ou non.. ainsi que les applications d'un tel procédé.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING |
| title | METHOD FOR ISOLATING EXOSOMES |
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