A METHOD OF IDENTIFYING GRAM-NEGATIVE BACTERIA USING THE MALDI-TOF MS METHOD
The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI...
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| creator | NIEDZIELA, TOMASZ KOJ, SABINA LUGOWSKI, CZESLAW |
| description | The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI-TOF spectrometer using DHB as a matrix. The subject invention is useful in the analysis of clinical samples, diagnosis of infections caused by Gram-negative bacteria and can be useful as a tool in the therapy directed against the O-antigen using specific antibodies.
La présente invention concerne l'identification de chimiotypes de lipopolysaccharides de bactéries à Gram négatif sur la base du profil d'endotoxine des bactéries entières à l'aide de MALDI-TOF MS. Le procédé issu de lipopolysaccharides (LPS) pour une préparation bactérienne est obtenu dans le mode négatif d'un spectromètre MALDI-TOF utilisant DHB comme matrice. La présente invention est utile dans l'analyse d'échantillons cliniques, le diagnostic d'infections provoquées par des bactéries à Gram négatif et peut être utile comme outil dans la thérapie dirigée contre l'antigène O à l'aide d'anticorps spécifiques. |
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La présente invention concerne l'identification de chimiotypes de lipopolysaccharides de bactéries à Gram négatif sur la base du profil d'endotoxine des bactéries entières à l'aide de MALDI-TOF MS. Le procédé issu de lipopolysaccharides (LPS) pour une préparation bactérienne est obtenu dans le mode négatif d'un spectromètre MALDI-TOF utilisant DHB comme matrice. La présente invention est utile dans l'analyse d'échantillons cliniques, le diagnostic d'infections provoquées par des bactéries à Gram négatif et peut être utile comme outil dans la thérapie dirigée contre l'antigène O à l'aide d'anticorps spécifiques.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140306&DB=EPODOC&CC=WO&NR=2014035270A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20140306&DB=EPODOC&CC=WO&NR=2014035270A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NIEDZIELA, TOMASZ</creatorcontrib><creatorcontrib>KOJ, SABINA</creatorcontrib><creatorcontrib>LUGOWSKI, CZESLAW</creatorcontrib><title>A METHOD OF IDENTIFYING GRAM-NEGATIVE BACTERIA USING THE MALDI-TOF MS METHOD</title><description>The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI-TOF spectrometer using DHB as a matrix. The subject invention is useful in the analysis of clinical samples, diagnosis of infections caused by Gram-negative bacteria and can be useful as a tool in the therapy directed against the O-antigen using specific antibodies.
La présente invention concerne l'identification de chimiotypes de lipopolysaccharides de bactéries à Gram négatif sur la base du profil d'endotoxine des bactéries entières à l'aide de MALDI-TOF MS. Le procédé issu de lipopolysaccharides (LPS) pour une préparation bactérienne est obtenu dans le mode négatif d'un spectromètre MALDI-TOF utilisant DHB comme matrice. La présente invention est utile dans l'analyse d'échantillons cliniques, le diagnostic d'infections provoquées par des bactéries à Gram négatif et peut être utile comme outil dans la thérapie dirigée contre l'antigène O à l'aide d'anticorps spécifiques.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2014</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPBxVPB1DfHwd1Hwd1PwdHH1C_F0i_T0c1dwD3L01fVzdXcM8QxzVXBydA5xDfJ0VAgNBkmGeLgq-Dr6uHjqhgC1-QZDzeBhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGhiYGxqZG5gaOhsbEqQIAK9kuSw</recordid><startdate>20140306</startdate><enddate>20140306</enddate><creator>NIEDZIELA, TOMASZ</creator><creator>KOJ, SABINA</creator><creator>LUGOWSKI, CZESLAW</creator><scope>EVB</scope></search><sort><creationdate>20140306</creationdate><title>A METHOD OF IDENTIFYING GRAM-NEGATIVE BACTERIA USING THE MALDI-TOF MS METHOD</title><author>NIEDZIELA, TOMASZ ; KOJ, SABINA ; LUGOWSKI, CZESLAW</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2014035270A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2014</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NIEDZIELA, TOMASZ</creatorcontrib><creatorcontrib>KOJ, SABINA</creatorcontrib><creatorcontrib>LUGOWSKI, CZESLAW</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NIEDZIELA, TOMASZ</au><au>KOJ, SABINA</au><au>LUGOWSKI, CZESLAW</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>A METHOD OF IDENTIFYING GRAM-NEGATIVE BACTERIA USING THE MALDI-TOF MS METHOD</title><date>2014-03-06</date><risdate>2014</risdate><abstract>The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI-TOF spectrometer using DHB as a matrix. The subject invention is useful in the analysis of clinical samples, diagnosis of infections caused by Gram-negative bacteria and can be useful as a tool in the therapy directed against the O-antigen using specific antibodies.
La présente invention concerne l'identification de chimiotypes de lipopolysaccharides de bactéries à Gram négatif sur la base du profil d'endotoxine des bactéries entières à l'aide de MALDI-TOF MS. Le procédé issu de lipopolysaccharides (LPS) pour une préparation bactérienne est obtenu dans le mode négatif d'un spectromètre MALDI-TOF utilisant DHB comme matrice. La présente invention est utile dans l'analyse d'échantillons cliniques, le diagnostic d'infections provoquées par des bactéries à Gram négatif et peut être utile comme outil dans la thérapie dirigée contre l'antigène O à l'aide d'anticorps spécifiques.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | A METHOD OF IDENTIFYING GRAM-NEGATIVE BACTERIA USING THE MALDI-TOF MS METHOD |
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